Molecular Heterogeneity of Canine Cutaneous Peripheral Nerve Sheath Tumors: A Drawback in the Diagnosis Refinement

Teixeira, Sílvia; Amorim, Irina; Rêma, Alexandra; Faria, Fátima; Gärtner, Fátima

doi:10.21873/invivo.11000

Cited by 11 publications

(18 citation statements)

References 31 publications

(53 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These data may be explained by the high proliferative rate of this tumour . This is also confirmed by the elevated expression of Ki‐67 protein, a widely used proliferation marker, in our COM cases as compared to other canine tumours such as mast cell tumours, indolent lymphoma, gastrointestinal stromal tumours, mammary tumours, peripheral nerve sheath tumours and gliomas …”

Section: Discussionsupporting

confidence: 83%

Cyclin D1 immunohistochemical expression and somatic mutations in canine oral melanoma

Zamboni

Brocca

Ferraresso

et al. 2019

Vet Comparative Oncology

View full text Add to dashboard Cite

Canine oral melanoma (COM) is the most frequent tumour with oral localization in dogs. Copy number gains and amplifications of CCND1, a gene coding for Cyclin D1, are the most frequent chromosomal aberrations described in human non-UV induced melanomas. Twenty-eight cases of COM were retrieved from paraffin-blocks archives. A total of 4 μm thick sections were immunostained with an antibody against human Cyclin D1 and Ki-67. Cyclin D1 and Ki-67 expressions were scored through two counting methods. DNA was extracted from 20 μm thick sections of formalin-fixed paraffin-embedded blocks. Pathological and surrounding healthy tissue was extracted independently. Cyclin D1 immunolabelling was detected in 69% (18/26) while Ki-67 was present in 88.5% (23/26) of cases. Statistical analysis revealed correlation between two counting methods for Cyclin D1 (r = 0.54; P = .004) and Ki-67 (r = 0.56; P = .003). The correlation found between Ki-67 and Cyclin D1 indexes in 16/26 cases labelled by both antibodies (r = 0.7947; P = .0002) suggests a possible use of Cyclin D1 index as prognostic marker. Polymerase chain reaction analysis on CCND1 coding sequence revealed the presence of nine somatic mutations in seven samples producing synonymous, missense and stop codons. Since none of the single-nucleotide polymorphisms was found to be recurrent, it is suggested that overexpression of Cyclin D1 may be the consequence of alterations of CCND1 upstream regions or other genetic aberrations not detectable with the methodology used in this study. Future studies are needed to verify the potential use of Cyclin D1 index as prognostic indicator and to highlight the molecular events responsible for Cyclin D1 overexpression in COMs. K E Y W O R D S CCND1, Cyclin D1, dog, immunohistochemistry, melanoma

show abstract

Section: Discussionsupporting

confidence: 83%

Cyclin D1 immunohistochemical expression and somatic mutations in canine oral melanoma

Zamboni

Brocca

Ferraresso

et al. 2019

Vet Comparative Oncology

View full text Add to dashboard Cite

show abstract

“…To date, there is no immunohistochemical marker that is expressed exclusively by PNSTs [20]. The immunohistochemical analysis of the 17 tumor samples in this study showed positivity for S-100 protein and glial fi brillary acidic protein in 13 cases.…”

Section: Discussionmentioning

confidence: 49%

“…Since the expression of S-100 protein may be present in some other tumors, even in those of non-neuronal origin (such as synovial sarcomas, leiomyosarcomas, and rhabdomyosarcomas), it should not be considered as defi nite proof of PNST [3,21]. However, the lack of S-100 protein expression is described in some cases of canine MPNSTs [3,19,20]. It is explained by the fact that MPNSTs consist of several cell types and S-100 protein expression will be strong only in tumors consisting predominantly of Schwann cells and a smaller number of perineural cells and fi broblasts [22].…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical Distinguishing between Canine Peripheral Nerve Sheath Tumors and Perivascular Wall Tumors

et al. 2019

View full text Add to dashboard Cite

Peripheral nerve sheath tumors (PNSTs) comprise a heterogeneous group of neoplasms originating from the elements of the nerve sheath. They are divided into two forms: benign and malignant PNST. Both benign and malignant PNSTs are not very common in domestic animals but they are reported in different animal species. Histologically, PNSTs are composed predominantly of spindle cells arranged in bundles, whorls and sheets, with a different number of pleomorphic cells and mitotic figures. The aim of this study was a reclassification of 17 dog tumor samples initially diagnosed with peripheral nerve sheath tumors using histopathological analysis. The main criterion for reclassification was immunohistochemical positivity for various antigens. PNSTs are often histologically very similar to other spindle cell tumors and immunohistochemistry is required for differential diagnosis. PNSTs generally express vimentin, S-100 protein, glial fibrillary acidic protein (GFAP), collagen IV and laminin. Four tumor samples were positive to muscular marker α-SMA and vimentin and negative for S-100 protein and desmin. The spindle cells whirling around some blood vessels were observed in these tumors so they were reclassified as perivascular wall tumors (PWTs). The other 13 tumors were S-100 protein and vimentin positive and α-SMA and desmin negative, thus classified as PNST. The use of the immunohistochemical panel is necessary for distinguishing PNSTs from PWTs in routine diagnostics.

show abstract

“…The survival time was shorter in human patients having Ki-67 index exceeding 25%. In canine cutaneous PNSTs, the expression of Ki-67 was ranged 0.6%-80.9%, showing higher index in MPNSTs (35.4+27.8%) than those in PMSTs (16.3+17%) [15]. The expression of Ki-67 in primary and metastatic lesion in the case were 34.6% and 26.5%, respectively, both of which were greater than 25%, implying that the MPNST in the case was highly aggressive.…”

mentioning

confidence: 83%

“…There are some variations, increasing (24%), stable (66%), and decreasing (10%) of Ki-67 in metastatic lesion in human neuroendocrine tumors [7]. Since Ki-67 can be used for grading or predicting prognosis in human PNSTs [18][19][20] and in canine PNSTs [15], Ki-67 is an available marker for physiological states and prognosis in MPNSTs. To our knowledge, this is the first report in humans or animals on the invasion of MPNSTs into the abdominal cavity from the vertebral canal through the nerve root.…”

mentioning

confidence: 99%

Primary malignant peripheral nerve sheath tumors arising from the spinal canal invading the abdominal cavity in a dog

Narita

Nishida

Goto

et al. 2020

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRAST. A 9-year-old neutered male Wire Fox Terrier presented with an 1-month history of hindlimb paresis. Magnetic resonance imaging revealed a contrast-enhanced mass at the level of the L2 vertebral canal. The dog became paraplegic with no deep perception of the hindlimbs, and the mass was surgically removed. The histopathological diagnosis was of a malignant peripheral nerve sheath tumor (MPNST). The dog suffered a relapse of right hindlimb ataxia at 225 days after the surgery. The dog died 434 days after the surgery. Necropsy found a large mass in the abdominal cavity invading from the L2-nerve. This is the first report of MPNST invading the abdominal cavity through the nerve root.

show abstract

Molecular Heterogeneity of Canine Cutaneous Peripheral Nerve Sheath Tumors: A Drawback in the Diagnosis Refinement

Abstract: Abstract. Background

Cited by 11 publications

References 31 publications

Cyclin D1 immunohistochemical expression and somatic mutations in canine oral melanoma

Cyclin D1 immunohistochemical expression and somatic mutations in canine oral melanoma

Immunohistochemical Distinguishing between Canine Peripheral Nerve Sheath Tumors and Perivascular Wall Tumors

Primary malignant peripheral nerve sheath tumors arising from the spinal canal invading the abdominal cavity in a dog

Contact Info

Product

Resources

About