Molecular Genotyping of Methicillin-ResistantStaphylococcus aureusStrains in a Teaching Hospital in Turkey

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the major causes of nosocomial infections in our hospital. Therefore, we aimed to characterize MRSA isolates phenotypically from patients with nosocomial infections at Cumhuriyet University Hospital between December, 1999, and June, 2001, in Sivas by analysis of antibiotic patterns and genotypically using pulsed-field gel electrophoresis (PFGE) and repetitive element sequence-based polymerase chain reaction (rep-PCR). Forty-three nosocomial isolates were collected from various wards. All isolates were resistant to penicillin, tetracycline, oxacillin, and gentamicin. By rep-PCR and by separation of SmaI fragments of genomic DNA using PFGE, one major type (eight subtypes with PFGE) was identified among the strains. This clone was found to be different than some clones such as Iberian, Brazilian, and a major clone that was found in another Turkish University Hospital in Ankara. According to our results, there is a major MRSA clone with a potential to spread in our hospital. Infection control measures should be directed toward restricting the further spread of this clone. Therefore, in accordance with these findings, a surveillance culturing program should be established.

Determination of SCCmec types in methicillin resistant staphylococci isolated from cows and farm workers

“…Elli dokuz izolatın 37 (%62.7)'si sığır ve 22 (%37.3)'si insan orjinliydi. 16S rRNA sekans analizi ile incelenen 59 MRS içinde 10 tür [S. haemolyticus (18), S. epidermidis (17), S. aureus (12), S. xylosus (4), S. cohnii (3), S. equorum (1), S. sciuri (1), S. succinus (1), S. pseudointermedius (1), S. saprophyticus (1) …”

“…Only one year later, Staphylococcus aureus isolates that showed resistance to methicillin were reported (8). Since then, methicillin resistance in staphylococci has appeared in countries worldwide in humans and continues to be one of the most common hospital-acquired pathogens (18,19,20).…”

“…Staphylococci play a role as nasocomial pathogens in human medicine, as bovine mastitis pathogens in veterinary medicine (5,18). Coagulase negative staphylococci (CoNS) have been recognized as bacteria of minor clinical importance; however, they may now be considered one of the factors that determines the generation of new MRS isolates (5, 7).…”

Genotyping of Methicillin Resistant Staphylococcus aureus Strains Causing Nosocomial Infections by Pulsed Field Gel Electrophoresis in Adana Çukurova University Hospital

“…2,3,9,10,[14][15][16] Co ok son ve ark. 11 Av ru pa ül ke sin de ki hasta ne ler den 1981-1998 yıl la rı ara sın da izo le edi len 98 MRSA ile yap tık la rı ça lış ma so nu cun da PFGE yön te mi ile üç ve ya da ha az bant fark lı lı ğı gös te ren 34 tip tes pit et miş ve en sık gö rü len Tip 3'ü oluş-tu ran 24 izo la tın ye di fark lı ül ke den el de edil di ği -ni bil dir miş ler dir.…”

“…Si vas Cum hu ri yet Üni ver si te si has ta ne sin de no zo ko mi yal in fek si yo na yol açan 43 MRSA izo latı nı PFGE ve rep-PCR yön tem le ri ile ge no ti pik olarak in ce le miş, PFGE ile bir ma jor tip ve 8 sub tip ta nım la mış lar dır. 15 Za ra ko lu ve ark. da Ha cet te pe Üni ver si te si Eriş kin Has ta ne sin de 2002-2004 yıl ları ara sın da has ta ne en fek si yo nu et ke ni ola rak izo le edi len 109 MRSA izo la tı ile yap tık la rı ça lış ma so nun da, PFGE ile 13 fark lı klon bul duk la rı nı, ve izo lat la rın %80'inin "A" klo nu na ait ol du ğu nu bildir miş ler dir.…”