Molecular genetics, physiology and biology of self-incompatibility in Brassicaceae

Watanabe, Masao; Suwabe, Keita; Suzuki, Go

doi:10.2183/pjab.88.519

Cited by 48 publications

(34 citation statements)

References 134 publications

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“…Flowering plants have evolved exquisite systems for the control of mating interactions before fertilization, and the most extensively studied of these is self-incompatibility (SI) (Suwabe et al 2010, Watanabe et al 2012). SI is a genetic system for intraspecific pollen selectivity and is recognized as the most sophisticated system to prevent selfing (Bateman 1995).…”

Section: Introductionmentioning

confidence: 99%

Cell Type-Specific Transcriptome of Brassicaceae Stigmatic Papilla Cells From a Combination of Laser Microdissection and RNA Sequencing

Osaka

Matsuda

Sakazono

et al. 2013

Plant and Cell Physiology

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Pollination is an early and critical step in plant reproduction, leading to successful fertilization. It consists of many sequential processes, including adhesion of pollen grains onto the surface of stigmatic papilla cells, foot formation to strengthen pollen–stigma interaction, pollen hydration and germination, and pollen tube elongation and penetration. We have focused on an examination of the expressed genes in papilla cells, to increase understanding of the molecular systems of pollination. From three representative species of Brassicaceae (Arabidopsis thaliana, A. halleri and Brassica rapa), stigmatic papilla cells were isolated precisely by laser microdissection, and cell type-specific gene expression in papilla cells was determined by RNA sequencing. As a result, 17,240, 19,260 and 21,026 unigenes were defined in papilla cells of A. thaliana, A. halleri and B. rapa, respectively, and, among these, 12,311 genes were common to all three species. Among the17,240 genes predicted in A. thaliana, one-third were papilla specific while approximately half of the genes were detected in all tissues examined. Bioinformatics analysis revealed that genes related to a wide range of reproduction and development functions are expressed in papilla cells, particularly metabolism, transcription and membrane-mediated information exchange. These results reflect the conserved features of general cellular function and also the specific reproductive role of papilla cells, highlighting a complex cellular system regulated by a diverse range of molecules in these cells. This study provides fundamental biological knowledge to dissect the molecular mechanisms of pollination in papilla cells and will shed light on our understanding of plant reproduction mechanisms.

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Section: Introductionmentioning

confidence: 99%

Cell Type-Specific Transcriptome of Brassicaceae Stigmatic Papilla Cells From a Combination of Laser Microdissection and RNA Sequencing

Osaka

Matsuda

Sakazono

et al. 2013

Plant and Cell Physiology

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“…This higher expression of non-enzymatically active ISA2 and low expression of enzymatically active ISA1 resembles the expression of cell-cell recognition genes, SLG and SRK, of the self-incompatibility system in Brassica species (Watanabe et al 2012). The S-domain of SRK, the receptor for the pollen ligand SP11, is highly similar to SLG, and SRK interacts with SP11 but SLG does not.…”

Section: Spatiotemporal Expression Of Ibisa Genes During Tuberous Roomentioning

confidence: 76%

Molecular characterization of genes encoding isoamylase-type debranching enzyme in tuberous root of sweet potato, <i>Ipomoea batatas</i> (L.) Lam.

Nabemoto

Watanabe

Mizuho

et al. 2016

Plant Biotechnology

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Isoamylase (ISA) is a starch debranching enzyme that removes α-1,6-glucosidic linkages in α-polyglucans such as amylopectin. From previous studies, plant isoamylases have been shown to play a crucial role in amylopectin biosynthesis; however, little is known about their function in storage root tissues of plants such as cassava, yam and sweet potato. In this study, we isolated cDNA clones and characterized the cDNA nucleotide sequences of three genes (IbISA1, IbISA2, IbISA3) encoding isoamylase from sweet potato (Ipomoea batatas (L.) cv. White Star). Deduced amino acid sequences of the three isolated IbISAs have the specific regions that are highly conserved among the α-amylase family members. The product of IbISA2 is predicted to be enzymatically inactive, like other plant ISA2s, due to replacement of amino acid residues that are important for hydrolytic reaction. qRT-PCR analysis demonstrated that expression of IbISA2 was higher than that of the other two IbISAs (IbISA1 and IbISA3) in tuberous root at 109 days after planting, at which stage of tuberous root was at which stage tuberous roots were almost fully developed almost developed. This expression pattern observed in our experiments was different from that in other sink organs, such as seeds (endosperms), indicating that orchestration of ISA gene expression may depend on the differences in sink organ type between tuberous roots and seeds. The molecular characterization of three IbISA genes and their expression analysis in this study will contribute to further studies on starch biosynthesis in sweet potato, especially in storage root.

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“…Phosphorylated SRK interacts with MLPK. After the subsequent signal transduction, which has not yet been determined, rejection of the self-pollen occurs (Watanabe et al 2012). (Murase et al 2004;Kakita et al 2007a).…”

Section: Mechanisms Involved In the Si Reactionmentioning

confidence: 99%

“…(B) In the dominant S-allele, small RNA, termed Smi (SP11 methylation inducer), is specifically produced, and its nucleotide sequence is highly similar to the promoter region of the recessive SP11 gene. This small RNA induces the methylation of recessive SP11, and represses the recessive SP11 at the transcriptional level (Watanabe et al 2012).…”

Section: Dominance Relationships Between S Allelesmentioning

confidence: 99%

Molecular Genetic Aspects of Self-incompatibility in Brassicaceae

Jung¹,

Ahmed²,

Park³

et al. 2013

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Department of Crop Science, Chungbuk National University, 52 Naesudongro, Heungdokgu, Cheongju 361-763, Republic of Korea ABSTRACT Molecular genetic studies of self-incompatibility (SI) are the most accentuating part in the way of advancement of reproductive mechanisms in flowering plants. In the Brassicaceae plants, self-incompatibility has been mapped genetically to a single chromosomal location where several closely linked genes have been identified. Recently, various studies have provided a novel insight into the basis of specificity in the S-receptor kinase (SRK) and S-locus protein 11 or S-locus Cysteine-rich (SP11/SCR) interaction, the nature of the signaling cascade that culminates in the inhibition of 'self' pollen, and the physiological and morphological changes that are associated with transitions between the outbreeding and inbreeding modes of mating in the Brassicaceae. In this review, we discuss the current view of the molecular genetic aspects of the self-incompatibility in Brassicaceae.

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Molecular genetics, physiology and biology of self-incompatibility in Brassicaceae

Cited by 48 publications

References 134 publications

Cell Type-Specific Transcriptome of Brassicaceae Stigmatic Papilla Cells From a Combination of Laser Microdissection and RNA Sequencing

Cell Type-Specific Transcriptome of Brassicaceae Stigmatic Papilla Cells From a Combination of Laser Microdissection and RNA Sequencing

Molecular characterization of genes encoding isoamylase-type debranching enzyme in tuberous root of sweet potato, <i>Ipomoea batatas</i> (L.) Lam.

Molecular Genetic Aspects of Self-incompatibility in Brassicaceae

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