Molecular genetics of cytoplasmic male sterility and restorer-of-fertility for the fine tuning of pollen production in crops

Kitazaki, Kazuyoshi; Oda, Kotoko; Akazawa, Akiho; Iwahori, Ryoma

doi:10.1007/s00122-023-04398-8

Cited by 5 publications

References 159 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Two cytoplasmic male sterility phenotypes in beet (Beta vulgaris L.): implications of their simultaneous onset and divergent paths

Katsura,

Itoh,

Matsuhira

et al. 2023

Euphytica

Self Cite

View full text Add to dashboard Cite

Cytoplasmic male sterility (CMS) is a mitochondrion-encoded trait speci cally affecting anthers. Several male sterility-inducing mitochondrial types are known, many of which affect the development of anther tapetum cells, but no sound explanation for this tissue's vulnerability has been proposed. To determine whether different male sterility-inducing mitochondrial types of the same plant species have similar cellular phenotypes, we compared anther development in two sugar beet CMS lines. We compared an Owen type CMS line used for hybrid breeding with the G type CMS line derived from wild beet. Both CMS lines have the same nuclear background. The tapetum of the G CMS line exhibited hypertrophy in the microspore stage, as reported previously in Owen CMS lines. Ultrastructural analysis revealed mitochondrial abnormalities, including low electron density and aberrant cristae appearing in the tapetum after meiosis in both lines. The Owen CMS line lacked Ubisch bodies and had poorly developed bacula and tecta in the pollen cell walls, whereas the G CMS line retained these features, but the pollen wall was highly deformed. Ultimately, microspores and the tapetum degenerated in both lines, and the male sterile phenotypes were eventually very similar. Although it had been hypothesized that mitochondrial activation was associated with CMS expression, mitochondria in the root apical meristem appeared normal in beet roots with G-and Owen type mitochondria. We propose that CMS expression includes at least two mechanisms: one triggers abnormal mitochondrial generation, and the other affects the type of developmental abnormality.

show abstract

Two cytoplasmic male sterility phenotypes in beet (Beta vulgaris L.): implications of their simultaneous onset and divergent paths

Katsura,

Itoh,

Matsuhira

et al. 2023

Euphytica

Self Cite

View full text Add to dashboard Cite

show abstract

The cellulose synthase-like gene CaCslD1 is required for male fertility in pepper

Wang,

Duan,

Wei

et al. 2024

Scientia Horticulturae

View full text Add to dashboard Cite

The mitochondrial orf117Sha gene desynchronizes pollen development and causes pollen abortion in Arabidopsis Sha cytoplasmic male sterility

Dehaene,

Boussardon,

Andrey

et al. 2024

Journal of Experimental Botany

View full text Add to dashboard Cite

Cytoplasmic male sterility (CMS) is of major agronomical relevance in hybrid breeding. In gametophytic CMS, abortion of pollen is determined by the grain genotype, while in sporophytic CMS, it is determined by the mother plant genotype. While several CMS mechanisms have been dissected at the molecular level, gametophytic CMS has not been straightforwardly accessible. We used the gametophytic Sha-CMS in Arabidopsis to characterize the cause and process of pollen abortion by implementing in vivo biosensing in single pollen and mitoTALEN mutagenesis. We obtained conclusive evidence that orf117Sha is the CMS-causing gene, despite distinct characteristics from other CMS-genes. We measured the in vivo cytosolic ATP content in single pollen, followed pollen development and analyzed pollen mitochondrial volume in two genotypes that differed only by the presence of the orf117Sha locus. Our results show that the Sha-CMS is not triggered by ATP deficiency. Instead, we observed desynchronization of a pollen developmental program. Pollen death occurred independently in pollen grains at diverse stages and was preceded by mitochondrial swelling. We conclude that pollen death is grain-autonomous in Sha-CMS and propose that mitochondrial permeability transition, which was previously described as a hallmark of developmental and environmental-triggered cell death programs, precedes pollen death in Sha-CMS.

show abstract

Molecular genetics of cytoplasmic male sterility and restorer-of-fertility for the fine tuning of pollen production in crops

Cited by 5 publications

References 159 publications

Two cytoplasmic male sterility phenotypes in beet (Beta vulgaris L.): implications of their simultaneous onset and divergent paths

Two cytoplasmic male sterility phenotypes in beet (Beta vulgaris L.): implications of their simultaneous onset and divergent paths

The cellulose synthase-like gene CaCslD1 is required for male fertility in pepper

The mitochondrial orf117Sha gene desynchronizes pollen development and causes pollen abortion in Arabidopsis Sha cytoplasmic male sterility

Contact Info

Product

Resources

About