Molecular genetics of Cicindela (Cylindera) terricola and elevation of C. lunalonga to species level, with comments on its conservation status

Woodcock, M. Ryan; Kippenhan, Michael G.; Knisley, C. Barry; Foster, James A.

doi:10.1007/s10592-006-9233-7

Cited by 13 publications

(9 citation statements)

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“…The COI interspecific distance between O. eremita and O. cristinae (6.6%) was higher than the intraspecific distance observed within each species (~1%). For beetles, a value of 6% for COI differentiation between two observed lineages can be considered supportive for separating species (Woodcock et al 2007;Nakamine and Takeda 2008;Solano et al 2013). Our COI data thus confirm the COI gene as a reliable barcode marker for species-level identification in the genus Osmoderma (cfr.…”

Section: Discussionsupporting

confidence: 74%

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Assessing the taxonomic status ofOsmoderma cristinae(Coleoptera: Scarabaeidae), endemic to Sicily, by genetic, morphological and pheromonal analyses

Zauli

Carpaneto

Chiari

et al. 2016

J Zoolog Syst Evol Res

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Resolving complexes of closely related and cryptic insect species can be challenging, especially when dealing with rare and protected taxa that are difficult to collect for genetic and morphological analyses. Until recently, populations of the genus Osmoderma (Scarabaeidae), widespread in Europe, were treated as a single species O. eremita (Scopoli, 1763) in spite of observed geographic variation in morphology. A previous survey using sequence data from the mtDNA cytochrome C oxidase I gene (COI) revealed the occurrence of at least two distinct lineages within this species complex: O. eremita in the west and O. barnabita Motschulsky, 1845, in the east. Interestingly, beetles confined to Sicily have been described as a distinct species, O. cristinae Sparacio, 1994, based on morphological traits. Only few Sicilian specimens were included in the former genetic analysis, and the results led to a still questionable taxonomic rank for these populations. To explore the robustness of the previous taxonomic arrangement for O. cristinae, a combination of genetic, morphological and pheromonal analyses was used. A 617‐bp fragment of the COI gene, aligned with O. cristinae and O. eremita sequences already available in GenBank, showed a clear genetic divergence between the two species (interspecific mean distance = 6.6%). Moreover, results from AFLP markers sustained the distinction of the two species. In addition, geometric morphometric analyses of the shape of male genitalia revealed a clear differentiation between the two species. Via scent analysis and field trapping, we demonstrated the production of the sex pheromone (R)‐(+)‐γ‐decalactone by males of O. cristinae, the attraction by conspecific individuals (mostly females) to this compound, and a lack of antagonistic effect of (S)‐(–)‐γ‐decalactone. The fact that O. eremita and O. eremita use the same compound for mate finding suggests that this sex pheromone has not undergone a differentiation and probably the allopatry of these two species compensates for the absence of a mechanism to avoid cross‐attraction. Our genetic and morphological data support the divergence of the two species and confirm the species status for O. cristinae, while sex pheromones are confirmed to be invariant among different species of the genus Osmoderma.

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Section: Discussionsupporting

confidence: 74%

“…For beetles, a value of 6% for COI differentiation between two observed lineages can be considered supportive for separating species (Woodcock et al. ; Nakamine and Takeda ; Solano et al. ).…”

Section: Discussionmentioning

confidence: 99%

Assessing the taxonomic status ofOsmoderma cristinae(Coleoptera: Scarabaeidae), endemic to Sicily, by genetic, morphological and pheromonal analyses

Zauli

Carpaneto

Chiari

et al. 2016

J Zoolog Syst Evol Res

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“…Duckett and Kjer 2003;Hebert et al 2003Hebert et al , 2004Moritz and Cicero 2004). Comparisons of mtDNA studies find sequence divergence of less than 2% primarily on an intraspecific level (Vogler and DeSalle 1994;Avise 2000;Woodcock et al 2007). This is also the case among populations of another ground beetle species, Nebria rufescens, which are not formally distinguished at the species or subspecies level and display a mean mtDNA-sequence divergence (ND1) of 1.3% (Schmitt et al this book).…”

Section: Implications For Conservation and Future Workmentioning

confidence: 99%

Linking Genetics and Ecology: Reconstructing the History of Relict Populations of an Endangered Semi-Aquatic Beetle

et al. 2009

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Population ecology and genetic studies are complementary approaches to address central questions of conservation biology and can provide information for the protection of biodiversity and the improvement of conservation measures which may otherwise be unattainable. This contribution highlights the benefit of combining diverse approaches for obtaining knowledge on a relict species and for implementing suitable conservation measures. We use the example of Carabus variolosus, a flightless European beetle that is listed as a Species of Community Interest in the EU Habitats and Species Directive. Ongoing investigations established the species' habitat choice, population ecology and population genetics, and yield first results from mitochondrial DNA analysis (COI). In small isolated habitat patches we found small sized populations that are genetically strongly differentiated even if adjacent. Evidence for a number of glacial refugia was found. However, all C. v. nodulosus populations studied North of the Alps share the same haplotype. We will discuss our findings in the light of the history of the species, of its taxonomic classification and of their implications for conservation.

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“…Genetic diversity within a single species is a fundamental aspect of biodiversity and can be used in species conservation and management (Woodcock et al 2007, Frankham et al 2009, Todisco et al 2010, Solano et al 2013. Analyses of DNA polymorphisms offer high resolution and can resolve intraspecific patterning even within morphologically similar cryptic species (Hebert et al 2003b, Bickford et al 2007, Murray et al 2008, Huemer and Hebert 2011, Jusoh et al 2014, Solano et al 2016.…”

Section: Introductionmentioning

confidence: 99%

Evidence for geographic substructuring of mtDNA variation in the East European Hermit beetle (Osmoderma barnabita)

Landvik¹,

Miraldo²,

Niemelä³

et al. 2017

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The genus Osmoderma is a flagship taxon of invertebrate conservation in Europe and encompasses a complex of four accepted species. While species limits amongst Osmoderma have been intensively studied, patterns of intraspecific variation are poorly known. In this paper, the authors focus on clarifying the phylogeographic structure of the East European Osmoderma barnabita using samples from Croatia to Finland. Samples of hind legs were collected from populations in Latvia and Finland (n=186) and combined with previously-published sequences from GenBank and museum specimens (n=10). In a partial sequence of the mitochondrial COI gene (759 bp), 26 closely related haplotypes were found. Beetle samples from different parts of Europe were distinct and showed no overlap in haplotype composition. The solitary population of Finland proved to be monomorphic and all 97 individuals sampled here belonged to a single haplotype unique to this region. The results suggest the Northern parts of Eastern Europe to be dominated * These authors contributed equally to this work. RESEARCH ARTICLE Launched to accelerate biodiversity conservation A peer-reviewed open-access journalMatti Landvik et al. / Nature Conservation 19: 171-189 (2017) 172 by a single COI haplotype to which most of the other haplotypes are linked by one or two mutations. The pattern seems to reflect a founder effect or a strong bottleneck event. While O. barnabita is widely distributed over Eastern Europe, current patterns of mitochondrial genetic diversity appear influenced by population history and little homogenisation by ongoing gene flow. From a conservation perspective, the patterns suggest that regional populations might need to be managed as subunits and that the population of Finland may be affected by low genetic diversity.

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Molecular genetics of Cicindela (Cylindera) terricola and elevation of C. lunalonga to species level, with comments on its conservation status

Cited by 13 publications

References 50 publications

Assessing the taxonomic status ofOsmoderma cristinae(Coleoptera: Scarabaeidae), endemic to Sicily, by genetic, morphological and pheromonal analyses

Assessing the taxonomic status ofOsmoderma cristinae(Coleoptera: Scarabaeidae), endemic to Sicily, by genetic, morphological and pheromonal analyses

Linking Genetics and Ecology: Reconstructing the History of Relict Populations of an Endangered Semi-Aquatic Beetle

Evidence for geographic substructuring of mtDNA variation in the East European Hermit beetle (Osmoderma barnabita)

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