Molecular genetics for probiotic engineering: dissecting lactic acid bacteria

Suissa, Ronit; Oved, Rela; Jankelowitz, Gavi; Turjeman, Sondra; Koren, Omry; Kolodkin‐Gal, Ilana

doi:10.1016/j.tim.2021.07.007

Cited by 42 publications

(35 citation statements)

References 105 publications

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“…Out of the 80 sampled patents, ~49% ( n = 39) specified one or multiple probiotic strains and out of 62 patents, ~39% ( n = 24) specified a probiotic strain or product with traceable composition. The lack of specification can be considered a barrier in the research process, as the effects of a probiotic can differ vastly between strains ( Hill et al, 2014 ; Suissa et al, 2022 ), making it unclear which probiotic strain or strains are responsible for the reported health effects disclosed in patents and clinical trials, and negatively impacting the replicability of probiotic research.…”

Section: Discussionmentioning

confidence: 99%

“…The effects of probiotics can vary not only between strains but also within strains, for example, because of different dosages or characteristics of the user ( Suez et al, 2019 ). Moreover, probiotic species can exert their effects through different mechanisms; lactic acid bacteria, for example, can affect the gut microbiota through fermentation, communication via vesicles, as well as the production of antibiotics ( Suissa et al, 2022 ). On top of that, probiotic efficacy has been shown to be impacted by carrier matrices ( Flach et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

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Probiotics for the Management of Infectious Diseases: Reviewing the State of the Art

2022

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This review aims to provide insight into the potential of probiotics as a clinical modality targeted at infectious diseases by creating a comprehensive overview of the state of the art of research and development efforts as shown by patents and clinical trials of the past 20 years. Data were retrieved from patent and clinical trial databases to reflect the long- and short-term developments of probiotics research. The data were analyzed to extract information on the total number of patents and trials for each indication, application date and location, and applicant/sponsor type. A total of 80 infectious diseases were investigated, precipitating in 789 patents and 602 clinical trials for 67 indications studied as targets of probiotics. An increasing trend was seen for the number of patents and clinical trials that were applied for since 1999 with the highest number of patents and clinical trials targeted to digestive tract, respiratory, and urogenital indications. Overall, research demonstrated a substantial interest in probiotics targeting infectious diseases, which was in line with reported unmet needs and global probiotics sales estimates. However, the declining rate of translation from patents to clinical trials indicates that there are some barriers obstructing the research process.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Probiotics for the Management of Infectious Diseases: Reviewing the State of the Art

2022

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“…Therefore, we focused on the Lactic acid bacteria (LAB) with potent probiotics features and antibacterial activities against various intestinal and urinary tract infections developing pathogens. Nowadays, LABs are considered the most efficient probiotics with potent antimicrobial agents [26,27]. We isolated several LABs from whole crop rice and screened for their antimicrobial activity primarily and selected two LABs based on their microbial activities and identified them at genus and species levels.…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, antibacterial effects of isolated strains against E. coli, S. aureus, P. aeruginosa and E. faecalis were demonstrated by the agar well diffusion, microdilution, MIC/MBC, time-killing assay and co-culture methods. Several, studies have reported that the Lactobacillus strains exhibited significant antibacterial activities via the production of metabolites, competition with nutrients utilization, and inhibition of bacterial adhesion to the mucosa and improve the immune response [26,27]. In vitro studies also suggested that Lactobacillus lactis exhibited strong antibacterial activities against E. coli, S. aureus, P. aeruginosa, S. typhimurium and E. faecalis [41,42].…”

Section: Discussionmentioning

confidence: 99%

Isolation of Lactococcus lactis from Whole Crop Rice and Determining Its Probiotic and Antimicrobial Properties towards Gastrointestinal Associated Bacteria

Ilavenil

Yoon²,

Muthusamy

et al. 2021

Microorganisms

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Antimicrobial resistance is an emerging condition that increases the risk of spreading and prolonging infectious diseases globally. Therefore, a new alternative strategy for antibiotics is required urgently to control pathogens spreading. Probiotics are considered as an alternative for antibiotics that inhibit pathogens. In the present study, potent lactic acid bacteria (LAB) were isolated and screened for their probiotic characteristics and antagonistic activity against intestinal pathogens by agar well diffusion, Time and Dose-dependent killing assay, minimum inhibitor, and minimum bactericidal concentration (MIC/MBC), and co-culture methods. The Lactococcus lactis RWP-3 and RWP-7 fermented the different carbohydrate substrates and produced different extracellular enzymes. Both isolates showed significant tolerant capability in the gastric, duodenal, and intestinal juices. In addition, RWP-3 and RWP-7 had hydrophobicity and aggregation properties in a time-dependent manner. Furthermore, the cell-free secondary metabolites (CFS) of RWP-3 and RWP-7 showed strong antibacterial activity against Escherichia coli,Staphylococcus aureus, Pseudomonas aeruginosa and Enterococcus faecalis. A co-culture study revealed that the RWP-3 and RWP-7 strongly compete with pathogen growths. RWP-3 and RWP-7 showed strong antagonistic activities against tested pathogens with significant probiotic characteristics, suggesting that these strains obtained could be used as an alternative strategy for the antibiotic to control infectious pathogens.

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“…Bacillus subtilis and related Bacillus species, such as B. amyloliquefaciens, B. velezensis and B. mojavensis, serve as biocontrol agents, plant and mammalian probiotics [1][2][3] . Beneficial Bacillus species protect their hosts from both fungal and bacterial pathogens [4][5][6] , a protection that is mediated, in part, by the formation of biofilms 7,8 , as well as by the production of a wide range of antibiotics 2,9 .…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis thaliana induces multigenerational stress tolerance against biotic and abiotic stressors and memorization of host colonization in Bacillus subtilis

Gilhar

Olender

Aharoni

et al. 2022

Preprint

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Bacillus subtilis is a beneficial bacterium that supports plant growth and protects it from bacterial, fungal, and viral infections. Here using a simplified system of B. subtilis, and Arabidopsis thaliana interactions, we found that history-dependent behavior is a potentially important manifestation of host colonization, worth classifying and quantifying. To study history-dependent adaptation to plant hosts, we develop a simple framework for measuring the physiological memory of B. subtilis following its interaction with Arabidopsis thaliana. We found that A. thaliana secretions reduce the lag time in pre-exposed bacteria compared with unexposed B. subtilis cells, even after their complete removal. Pre-exposed B. subtilis cells colonized plant roots more efficiently than unexposed bacteria, and were more resistant to biotic and abiotic stressors such as salicylic acid, and high salinity. Descendants of bacteria treated with plant secretions had an advantage in the competition against unexposed bacteria for root colonization. The effect of plant secretions was independent of their roles as nitrogen and carbon sources. Transcriptome analysis of both ancestors and descendants revealed that a specific set of plant-induced processes, among them c-di-AMP homeostasis, and the general stress response, maintain the signature of association with the plant in descendants of pre-exposed bacteria. Consistently, plant secretions compensated for the loss of c-di-AMP cyclases but required the general stress response and the master regulator Spo0A to exert their short and long-term effects.

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Molecular genetics for probiotic engineering: dissecting lactic acid bacteria

Cited by 42 publications

References 105 publications

Probiotics for the Management of Infectious Diseases: Reviewing the State of the Art

Probiotics for the Management of Infectious Diseases: Reviewing the State of the Art

Isolation of Lactococcus lactis from Whole Crop Rice and Determining Its Probiotic and Antimicrobial Properties towards Gastrointestinal Associated Bacteria

Arabidopsis thaliana induces multigenerational stress tolerance against biotic and abiotic stressors and memorization of host colonization in Bacillus subtilis

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