Molecular Genetic Typing ofLegionella pneumophilaStrains Isolated in Jejudo, Korea

Abstract

Twenty two strains of Legionella species isolated from Jejudo, Korea were identified by comparing the rpoB (300 bp), dotA (360 bp), and mip (396 bp) gene sequence analysis. Furthermore, their genotypes were determined by sequence analysis of rpoB/dotA subgroup typing, pulse-field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD) patterns. Of the 22 isolates, 21 strains were identified as L. pneumophila and 1 strain was close to L. erythra (>95% similarity of rpoB and mip). Most of the L. pneumophila strains (90%) belonged to P-I of rpoB/dotA subgroup typing, one strain of each P-III and P-IV. L. pneumophila isolates were further grouped into 4 and 6 different PFGE (P1 to P4) and RAPD (R1 to R6) patterns, respectively. On the basis of these genotypes, which may be useful for future epidemiological studies, existence of diverse L. pneumophilla population in Jejudo, Korea were observed.

Rice and Social Differentiation on a Volcanic Island: An Archaeobotanical Investigation of Yerae-dong, Korea

“…Ethnographic work by Japanese anthropologist Seiichi Izumi in rural Jejudo villages in the 1930s documented that rice was a luxury food. It was eaten only to celebrate the completion of communal work, or to serve special guests, whereas staple crops were barley and millets (J. Kim 2014). The extant historic texts on Jejudo, albeit few in number, consistently state that rice was a rare and highly prized food, demonstrating that people had access to rice in a manner directly proportional to their political and economic power.…”