Molecular genetic study of potentially bacteriocinogenic clinical and dairy Enterococcus spp. isolates from Bulgaria

Dimov, Svetoslav; Atanasova, Д.; Petkova, V.; Savov, Encho; Mitov, Ivan

doi:10.1007/s13213-015-1120-3

Cited by 4 publications

(12 citation statements)

References 25 publications

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“…diacetylactis were dominant isolates from raw‐milk Arzúa‐Ulloa cheeses (Menéndez, Centeno, Godinez, & Rodriguez‐Otero, ), which is in accordance with our research. Giraffa () and Strateva et al () noticed that, among enterococci, E. faecium and E. faecalis were generally the most frequent and prevalent species isolated from cheeses, which was also confirmed in our paper.…”

Section: Discussionsupporting

confidence: 90%

Characterization of lactic acid bacteria isolated from traditionally made Serbian Cheese and evaluation of their antagonistic potential against Enterobacteriaceae

Muruzović

Mladenović

Žugić-Petrović³

et al. 2018

J Food Process Preserv

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In this article, the presence and physiological characteristics of lactic acid bacteria (LAB) isolated from traditionally made cheese from Southeastern Serbia were examined. Three samples, from different households, were obtained in spring. The screening of antagonistic potentials, using agar‐well diffusion method, was evaluated. Briefly, three different genera of LAB (Enterococcus [32%], Lactococcus [53%], and Lactobacillus [15%]) were identified. The members of the genus Enterococcus were Enterococcus faecium and Enterococcus faecalis. The member of genus Lactococcus was Lactococcus lactis subsp. lactis var. diacetylactis. The members of genus Lactobacillus were Lactobacillus fermentum, Lactobacillus plantarum, and Lactobacillus paracasei subsp. paracasei. The antagonism of LAB on the growth of Escherichia coli ATCC 25922, Proteus mirabilis ATCC 12453, Klebsiella oxytoca KGPMF1, Klebsiella ornithinolytica KGPMF8, and Aeromonas hydrophila was examined. All tested isolates inhibited the growth of at least one indicator strain with growth inhibition zone from 10 to 21 mm. Results indicated that LAB demonstrated the potential of food biopreservation. Practical applications Traditionally made cheeses represent excellent source of unexplored microflora, especially of autochthonous lactic acid bacteria (LAB). In cheese from Sokobanja, the isolated LAB genera were Lactobacillus sp., Lactococcus sp., and Enterococcus sp. Also, the physiological activity of LAB, as well as their interaction with enterobacteria, which also was isolated from the mentioned cheese, was investigated. The results of this study demonstrated potential of LAB to interact agonistically with the growth of enterobacteria, which was important due to the well‐known fact that enterobacteria could affect the organoleptic properties of cheese. LAB showed potential of food biopreservation. Further investigation should include antagonistic potential related to food‐borne bacteria and the mechanisms of action.

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Section: Discussionsupporting

confidence: 90%

Characterization of lactic acid bacteria isolated from traditionally made Serbian Cheese and evaluation of their antagonistic potential against Enterobacteriaceae

Muruzović

Mladenović

Žugić-Petrović³

et al. 2018

J Food Process Preserv

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“…Although EntB was first isolated from E. faecium T136 of a Spanish dry sausage, it has also been found in cheese samples and among clinical isolates [55,56,58]. Freitas et al (2016) and Strateva et al (2015) described the presence of EntB as infrequent in VREfm and VSEfm clinical isolates [56,57]. Regarding the first study, the VREfm isolates harboring EntB were mostly from Latin America, thus suggesting the possibility of a regional spread of particular clones (EntB was occasionally located on the chromosome) or plasmids [56].…”

Section: Class Ii-other Bacteriocinsmentioning

confidence: 99%

“…Although there is a clear lack of surveillance studies analyzing the distribution of this and other enterocins among enterococci, especially from clinical samples, Freitas et al (2016) reported a high incidence of EntA in outbreak VREfm and vancomycin-susceptible E. faecium (VSEfm) obtained from hospitalized patients in different countries over the last decades [56]. Likewise, Strateva et al (2015) reported a high incidence of EntA in clinical E. faecium isolates from various Bulgarian hospitals [57]. Indeed, EntA has been suggested to be part of the core genome of E. faecium [58], being chromosomally located, suggesting it may provide a competitive niche advantage to this species.…”

Section: Class Iia-the Pediocin-like Bacteriocinsmentioning

confidence: 99%

“…Yet, a few studies also reported the presence of EntP in clinical isolates, although at low rates. Freitas et al (2016) detected the presence of EntP in VREfm and VSEfm isolates obtained from hospitalized patients in different countries, whereas Strateva et al (2015) identified EntP production in isolates from inpatients and outpatients from Bulgarian hospitals, suggesting it could contribute to enterococci virulence or colonization ability of the human host [56,57].…”

Section: Class Iia-the Pediocin-like Bacteriocinsmentioning

confidence: 99%

“…Enterolysin A was described in E. faecalis LMG 2333 isolated from Iceland fish, in E. faecalis DPC5280 extracted from an Irish raw milk sample, and also in an E. faecalis strain isolated from artisanal cheeses [57,88,89]. It has a broad spectrum of antimicrobial activity against Gram-positive bacteria, namely E. faecalis, L. innocua, L. lactis strains, among others (Table S1).…”

Section: Class Iii-bacteriolysinsmentioning

confidence: 99%

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Enterococcus spp. as a Producer and Target of Bacteriocins: A Double-Edged Sword in the Antimicrobial Resistance Crisis Context

et al. 2021

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Enterococcus spp. are one of the most frequent producers of bacteriocins (enterocins), which provides them with an advantage to compete in their natural environment, which is the gut of humans and many animals. The enterocins’ activity against microorganisms from different phylogenetic groups has raised interest in Enterococcus spp. in different contexts throughout the last decades, especially in the food industry. Nevertheless, some species can also cause opportunistic life-threatening infections and are frequently multidrug-resistant (MDR). Vancomycin-resistant Enterococcus (VRE), in particular, are an ongoing global challenge given the lack of therapeutic options. In this scenario, bacteriocins can offer a potential solution to this persistent threat, either alone or in combination with other antimicrobials. There are a handful of studies that demonstrate the advantages and applications of bacteriocins, especially against VRE. The purpose of this review is to present a current standpoint about the dual role of Enterococcus spp., from important producers to targets needed to be controlled, and the crucial role that enterocins may have in the expansion of enterococcal populations. Classification and distribution of enterocins, the current knowledge about the bacteriocinome of clinical enterococci, and the challenges of bacteriocin use in the fight against VRE infections are particularly detailed.

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Association between virulence factors and biofilm formation in Enterococcus faecalis isolated from semen of infertile men

Shahroodian

Mirshekar

Talebi

et al. 2022

American J Rep Immunol

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Problem: Enterococcus faecalis is a common microbial semen contaminant. Although virulence factors and biofilm formation have often been analyzed in Enterococcus spp., there is little information about these features in isolates obtained from the genitourinary tract. This study was intended to characterize and determine the relationship between biofilm-forming ability and the presence of E. faecalis virulence factors isolated from human semen. Method of study:A total of 32 patients diagnosed with primary infertility and 28 healthy men were included in the study. Semen analyses were performed according to the WHO guidelines. PCR reactions were applied for the detection of ace, esp, efeA, gelE, asa1, and cylA genes. Microtiter plate assay, via measurement of OD 560, was used to measure the biofilm-forming ability of the isolates.Results: Sixty E. faecalis isolates from semen of infertile and fertile men were characterized by phenotypic and genotypic methods. The prevalence of ace, esp, efeA, gelE, asa1 and cylA were reported to be 81.3%/100.0%, 81.3%/89.3%, 81.3%/85.7%, 71.9%/53.6%, 8.8%/75.0%, and 62.5%/67.9% in infertile/fertile groups; respectively. Strong, weak, and non-biofilm reactions were reported to be 50.0%/21.4%, 40.6%/64.3%, and 9.4%/14.3% in infertile and fertile groups; respectively. Conclusions:There was a significant relationship between fertility and weak biofilm reaction and also between biofilm formation and possession of the esp gene (P < .05).It could be speculated that colonization with E. faecalis with a strong ability for biofilm formation could become a potential threat to men's fertility.

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Molecular genetic study of potentially bacteriocinogenic clinical and dairy Enterococcus spp. isolates from Bulgaria

Cited by 4 publications

References 25 publications

Characterization of lactic acid bacteria isolated from traditionally made Serbian Cheese and evaluation of their antagonistic potential against Enterobacteriaceae

Characterization of lactic acid bacteria isolated from traditionally made Serbian Cheese and evaluation of their antagonistic potential against Enterobacteriaceae

Enterococcus spp. as a Producer and Target of Bacteriocins: A Double-Edged Sword in the Antimicrobial Resistance Crisis Context

Association between virulence factors and biofilm formation in Enterococcus faecalis isolated from semen of infertile men

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