Molecular Genetic Manipulation of Microalgae: Principles and Applications

Shrestha, Ranjit; Haerizadeh, Farzad; Hildebrand, Mark

doi:10.1002/9781118567166.ch10

Cited by 8 publications

(8 citation statements)

References 172 publications

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“…Briefly, the constitutive expression vector pTpfcpGFP (Poulsen et al 2006) was modified by cloning a Gateway frame B cassette upstream of egfp creating a destination vector. In cases where higher expression levels were required for in vivo localization, another destination vector was created utilizing the regulatory elements that drive constitutive expression of the ribosomal protein L41 (Shrestha et al 2013) instead of FCP. Native expression constructs were created by incorporating 1000 bp of the 5' flanking region and 500 bp of the 3' flanking region of a gene of interest into the pMHL_71 vector to provide the native regulatory elements as described in Shrestha and Hildebrand (2015).…”

Section: Bioinformatic Analysesmentioning

confidence: 99%

Clarification of Photorespiratory Processes and the Role of Malic Enzyme in Diatoms

Davis

Abbriano

Smith

et al. 2017

Protist

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Evidence suggests that diatom photorespiratory metabolism is distinct from other photosynthetic eukaryotes in that there may be at least two routes for the metabolism of the photorespiratory metabolite glycolate. One occurs primarily in the mitochondria and is similar to the C2 photorespiratory pathway, and the other processes glycolate through the peroxisomal glyoxylate cycle. Genomic analysis has identified the presence of key genes required for glycolate oxidation, the glyoxylate cycle, and malate metabolism, however, predictions of intracellular localization can be ambiguous and require verification. This knowledge gap leads to uncertainties surrounding how these individual pathways operate, either together or independently, to process photorespiratory intermediates under different environmental conditions. Here, we combine in silico sequence analysis, in vivo protein localization techniques and gene expression patterns to investigate key enzymes potentially involved in photorespiratory metabolism in the model diatom Thalassiosira pseudonana. We demonstrate the peroxisomal localization of isocitrate lyase and the mitochondrial localization of malic enzyme and a glycolate oxidase. Based on these analyses, we propose an updated model for photorespiratory metabolism in T. pseudonana, as well as a mechanism by which C2 photorespiratory metabolism and its associated pathways may operate during silicon starvation and growth arrest.

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Section: Bioinformatic Analysesmentioning

confidence: 99%

Clarification of Photorespiratory Processes and the Role of Malic Enzyme in Diatoms

Davis

Abbriano

Smith

et al. 2017

Protist

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“…In some cases, facilities could be placed conveniently close to the sources of inputs, for example close to a cement plant (as a source of CO 2 ) or a waste‐water treatment plant (as a source of water and nutrients) . The use of waste water throughout the process seems to be promising way to improve the environmental and economic sustainability of algae cultivation …”

Section: Review Of Existing Studiesmentioning

confidence: 99%

Evaluating microalgae‐to‐energy ‐systems: different approaches to life cycle assessment (LCA) studies

Collotta

Busi

Champagne

et al. 2016

Biofuels Bioprod Bioref

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Life cycle assessment (LCA) is a valuable tool for determining the environmental impacts associated with different products and has been widely used to assess biofuel production. As a scientific methodology rather than a standardized test, every LCA may be thought of as unique in terms of the selection of functional units or determination of system boundaries. Researchers generally tailor the method to meet the specific goals of their own investigations. This review examines a number of LCAs used to evaluate microalgae-to-energy systems, and evaluates their contributions in terms of their ability to support commercialization efforts in this sector. To this end, a new scoring system for LCAs is proposed based on input/output flows, data origin, production technologies and system boundaries, selection of assumptions and variables, as well as the ability to track environmental, economic, and social impacts. The review suggests that, while a wide variety of new technological pathways for microalgae-to-energy systems are being assessed, the majority of studies reported employ relatively limited system boundaries that may not capture the full impacts of the processes. The number of environmental impact factors being tracked is limited, and many studies do not consider important impacts such as water or land use. Most studies do not incorporate critical information about economics related to new process configurations, which will be essential to support commercialization efforts in this area. © 2016 Society of Chemical Industry and John Wiley & Sons, Lt

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“…The most frequently used technique for microalgal gene delivery is the gene gun [9]. While effective, this method is labor intensive, needs specialized costly equipment, and shows high variance in the efficiency of the delivered gene [8,9].…”

Section: Introductionmentioning

confidence: 99%

“…While effective, this method is labor intensive, needs specialized costly equipment, and shows high variance in the efficiency of the delivered gene [8,9]. Conventional alternative approaches are electroporation, sonication and glass bead/silicon carbide whisker agitation.…”

Section: Introductionmentioning

confidence: 99%

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Template-synthesized gold microneedle arrays for gene delivery to the Chlamydomonas reinhardtii chloroplast

et al. 2015

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Molecular Genetic Manipulation of Microalgae: Principles and Applications

Cited by 8 publications

References 172 publications

Clarification of Photorespiratory Processes and the Role of Malic Enzyme in Diatoms

Clarification of Photorespiratory Processes and the Role of Malic Enzyme in Diatoms

Evaluating microalgae‐to‐energy ‐systems: different approaches to life cycle assessment (LCA) studies

Template-synthesized gold microneedle arrays for gene delivery to the Chlamydomonas reinhardtii chloroplast

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