Molecular genetic identification of viruses affecting pepper crop (Capsicum spp.) in Western North of Benin

Abstract

A. A. Fanou et al. (2019) / Molecular genetic identification of viruses affecting pepper crop (Capsicum spp.

Molecular Identification of <em>Xanthomonas oryzae pv. oryzae</em> and Bacterial Leaf Blight Resistance Genes Xa5, and Xa21 in Cultivated Rice Accessions Benin

“…Polymerase chain reactions (PCR) were performed to identify resistance gene(s) among the selected accessions. Six co-segregating STS markers tightly linked to xa5, Xa7, xa13, and Xa21 [26][27][28][29] were used (Table 3) for the detection of bacterial blight-resistant gene(s). Amplification was carried out in a thermal cycler according to the following programme: pre-denaturation at 95°C for 5min followed by 35 cycles consisting of denaturation at 94°C for 1min, hybridisation at 51°C (for the RM122 marker), 59°C (for the M5 and xa13-prom markers), 55°C and 57°C (for the pTA248 marker) for1min, elongation at 72°C for 2min and final extension at 72°C for 5min.…”

Molecular Screening of Rice Cultivated in Benin for the Identification of &lt;i&gt;Xanthomonas oryzae&lt;/i&gt; Pv. &lt;i&gt;oryzae&lt;/i&gt; and Bacterial Leaf Blight Resistance Genes

“…since previous studies have identified a sequence of approximately 270 Pb which corresponds to a sequence specific to Sphingomonas sp. The technique used is based on the PCR protocol described by Fanou et al[27]. PCR was performed using a thermal cycler in 20 µL volumes containing a mixture of 1 × PCR buffer, 0.1 mM dNTPs, 0.2 μM each of forward and reverse primers, 50 -200 ng genomic DNA, 2.0 -3.0 U of Taq DNA polymerase, with sterilized distilled water added to 50 μL.…”