Molecular-genetic identification of arbuscular mycorrhiza fungi from Teberda natural reserve

Kryukov, Alexey A.; Gorbunova, Anastasia; Kurbanniyazov, Sh. K.; Mikhaylova, Yu. V.; Rodionov, A. V.; Шишова, М. Ф.; Zhurbenko, Peter M.; Yurkov, Andrey P.

doi:10.28983/plamic2020.134

Cited by 2 publications

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“…Identification the AMF strains with the highest symbiosis efficiency is of great value for agricultural applications. To do it, we isolated, identified (Kryukov et al, 2017;Kryukov, Yurkov, 2018;Yurkov et al, 2018b), and evaluated the symbiotic efficiency (Yurkov et al, 2017a, b;Yurkov et al, 2018a) for a number of strains from the collection.…”

Section: Introductionmentioning

confidence: 99%

Perspectives of using Illumina MiSeq for identification of arbuscular mycorrhizal fungi

et al. 2020

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Arbuscular mycorrhiza fungi (AMF) form one of the most common symbiosis with the majority of land plants. AMF supply the plant with various mineral elements, primarily phosphorus, and improve the water supply. The search for the most effective AMF strains for symbiosis and the creation of microbial preparations on that basis is an important task for modern biology. Owing to the difficulties of cultivation without a host plant and their high genetic polymorphism, identifying AMF is very difficult. A high number of cryptic species often makes morphological identification unreliable. Recent years have seen a growth in the number of AMF biodiversity studies performed by modern NGS-based methods, Illumina MiSeq in particular. Currently, there are still many questions that remain for the identification of AМF. The most important are whether conservative or variable sequences should be used to select a marker for barcoding and whether universal primers or those specific to AMF should be used. In our work, we have successfully used universal primers ITS3 and ITS4 for the sequencing in Illumina MiSeq of the 5.8S rDNA – ITS2 region of the 35S rRNA genes, which contain both a conservative and variable regions. The molecular genetic approach for AMF identification was quite effective and allowed us to reliably identify eight of nine isolates to the species level: five isolates of Rhizophagus irregularis, and one isolate of R. invermaius, Paraglomus laccatum, and Claroideoglomus etunicatum, respectively. For all five R. irregularis isolates, high variability in the ITS region and the absence of ecotopic-related molecular characters in the ITS2 region were demonstrated. The NCBI data is still insufficient for accurate AMF identification of Acaulospora sp. isolates from the genus to the species level.

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Section: Introductionmentioning

confidence: 99%

Perspectives of using Illumina MiSeq for identification of arbuscular mycorrhizal fungi

et al. 2020

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“…Inoculation of plants with adapted fungal populations during their cultivation on control substrates had differences: with Cr 2,5 it caused a significant increase in the content of photosynthetic pigments, and with Cr 10 , on the contrary, a significant decrease in their content. When cultivating plants on substrate with the addition of Cr 2,5 , there were 8th Scientific and Practical Conference 48±2,50 1 Here and hereinafter: the average value of the indicator ± standard deviation; 2 significant difference from control: increase ↑ or decrease ↓ of the indicator (p 0.05); Control − initial population that is not adapted to HM (corresponds to A0 in Fig. 1-3) and the substrate without metals (corresponds to B0 in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Certain successes have been achieved in studying the role of endomycorrhiza and its most common form -arbuscular mycorrhiza (AM), which is typical for most modern phylogenetic groups of plants and is introduced in all biomes of the globe. It is formed by fungi belonging to the Glomeromycotina subdivision of the Mucoromycota division [1]. But the use of AMF in crop production is limited, which is a consequence of their obligate symbiotrophy [2].…”

Section: Introductionmentioning

confidence: 99%

The Possibility of Using Endophytic Micromycetes for Increasing Plant Metal Resistance

Bukharina

Islamova

2022

KLS

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The aim of this research was to study the effect of inoculation with the Cylindrocarpon magnusianum endotrophic micromycete on the physiological and biochemical parameters of tomato test plants under the action of heavy metal salts. The plants were inoculated with the fungus culture (control population) and populations of this fungus preliminarily adapted to the action of the stress factor. Then, inoculated plants were grown under control conditions and on substrates with different concentrations of heavy metal salts (zinc, copper, lead and chromium). After the plants were inoculated with the control population of the C. magnusianum fungus, a stimulating effect increasing the plants’ resistance to the action of the heavy metal salts was not detected. When the plants were inoculated with adapted populations of the C. magnusianum fungus, adaptive reactions of the plants associated with the content of photosynthetic pigments in the leaves and the formation of plant biomass were significantly manifested. Under these conditions, a more intense development of fungal infection in plant roots was observed in contrast to the use of the control fungal population. These findings therefore demonstrated an effective partnership between the C. magnusianum fungus and the root system of plants under extreme conditions for plant life. Keywords: Cylindrocarpon magnusianum, micromycetes, heavy metals, inoculation, biochemical indicators

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Molecular-genetic identification of arbuscular mycorrhiza fungi from Teberda natural reserve

Abstract: Arbuscular mycorrhiza fungi of soil samples from North Caucasus were identified via Illumina Miseq and universal primers for ITS region. It was shown, that both ITS1 and ITS2 are necessary for identification.

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Perspectives of using Illumina MiSeq for identification of arbuscular mycorrhizal fungi

Perspectives of using Illumina MiSeq for identification of arbuscular mycorrhizal fungi

The Possibility of Using Endophytic Micromycetes for Increasing Plant Metal Resistance

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