Molecular genetic homogeneity assessment of micropropagated Dendrobium moschatum Sw. - A rare medicinal orchid, using RAPD and ISSR markers

Tikendra, Leimapokpam; Amom, Thoungamba; Nongdam, Potshangbam

doi:10.1016/j.plgene.2019.100196

Cited by 40 publications

(20 citation statements)

References 49 publications

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“…Total flavonoid content was measured using the aluminum chloride assay as described by Marinova et al [54] with some modifications. An aliquot (500 µL) of extracts or catechin standard (Sigma-Aldrich, St. Louis, MO, USA) solution (10,20,30,40,50, and 100 mg L −1 ) was added to a 5 mL Eppendorf tube, containing 2 mL distilled water. To the diluted sample, 150 µL of 5% sodium nitrite (AppliChem, Darmstadt, Germany) was added.…”

Section: Total Flavonoid Assaymentioning

confidence: 99%

“…The phenolic and flavonoid contents of the micropropagated plant leaves' extract were estimated spectrophotometrically in terms of gallic acid and catechin equivalence (GAE: gallic acid equivalent; CE: catechin equivalent) at 750 and 510 nm, respectively. Three replicates of different concentrations of gallic acid and catechin (10,20,30,40,50,100,150,200, and 300 µg ml −1 ) were used to deduce the standard curves for determination of phenolic and flavonoid content, respectively. The generated equation for the gallic acid standard curve was y = 0.0043x + 0.0019 R 2 = 0.9995 .…”

Section: Phenolic and Flavonoid Content Estimationmentioning

confidence: 99%

“…It is an efficient, quick, and reproducible technique in which the targets are the DNA fragments located between adjacent microsatellite regions, while the RAPD-PCR targets are random [18]. Wójcik et al [19] used ISSR primers to observe the genetic stability of regenerated plants of Ribes grossularia L. Both techniques are used together to obtain more realistic and accurate results [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

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Genetic Stability, Phenolic, Flavonoid, Ferulic Acid Contents, and Antioxidant Activity of Micropropagated Lycium schweinfurthii Plants

Mamdouh

Mahgoub

Gabr

et al. 2021

Plants

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Lycium schweinfurthii is a Mediterranean wild shrub rich in plant secondary metabolites. In vitro propagation of this plant may support the production of valuable dietary supplements for humanity, introduction of it to the world market, and opportunities for further studies. The presented study aimed to introduce an efficient and reproducible protocol for in vitro micropropagation of L. schweinfurthii and assess the genetic stability of micropropagated plants (MiPs) as well as to estimate phenolic, flavonoid, ferulic acid contents, and the antioxidant activity in leaves of micropropagated plants. Two DNA-based techniques, random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR), and one biochemical technique, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), were used to assess the genetic stability in MiPs. Spectrophotometric analysis was performed to estimate total phenolic and flavonoid contents and antioxidant activity of MiPs leaves, while ferulic acid content was estimated using high-performance thin-layer chromatography (HPTLC). Sufficient shoot proliferation was achieved at MS (Murashige and Skoog) medium supplemented with 0.4 mg L−1 kinetin and rooted successfully on half-strength MS medium fortified with 0.4 mg L−1 Indole-3-butyric acid (IBA). The Jaccard’s similarity coefficients detected in MiPs reached 52%, 55%, and 82% in the RAPD, ISSR, and SDS-PAGE analyses, respectively. In the dried leaves of MiPs, the phenolic, flavonoid, and ferulic acid contents of 11.53 mg gallic acid equivalent, 12.99 mg catechin equivalent, and 45.52 mg were estimated per gram, respectively. However, an IC50 of 0.43, and 1.99 mg mL−1 of MiP dried leaves’ methanolic extract was required to scavenge half of the DPPH, and ABTS free radicals, respectively. The study presented a successful protocol for in vitro propagation of a valued promising plant source of phenolic compounds.

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Section: Total Flavonoid Assaymentioning

confidence: 99%

Section: Phenolic and Flavonoid Content Estimationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic Stability, Phenolic, Flavonoid, Ferulic Acid Contents, and Antioxidant Activity of Micropropagated Lycium schweinfurthii Plants

Mamdouh

Mahgoub

Gabr

et al. 2021

Plants

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“…Principal coordinate analysis (PCoA) further confirmed the grouping of plants in accordance with the cluster analysis determined by unweighted pair group method using arithmetic averages (UPGMA) analysis. Tikendra et al (2019b) used Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) markers to assess genetic homogeneity assessment of micro-propagated Dendrobium moschatum. Analysis showed 95.2% and 98% genetic mono-morphism respectively among in vitro regenerants and the mother plant.…”

Section: Molecular Markers In Tissue Culturementioning

confidence: 99%

Evaluation of Secondary Metabolites in Wheat Grain (Triticum Sp.) Grown in Humid South Eastern Plain Zone of Rajasthan (India)

Chachaiya¹,

Mehta²

2019

IJAEB

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Understanding the genetics of floricultural germplasm represents a value added component of managing collections. Molecular approaches collectively represent a potential goldmine of important information that can be applied to programmes of genetic improvement. The development and utilization of molecular markers for the exploitation and identification of plant genetic diversity is one of the most key developments in the field of molecular genetics studies. Molecular markers are popular tool, due to their stability, cost-effectiveness and ease of use for a variety of applications in the field of molecular genetics. To know the role of molecular markers in genetic studies of floricultural crops, a number of molecular marker techniques have been reviewed by many researchers in worldwide. However, all these reviews were meant for researchers with advanced knowledge of molecular markers and their utility in limited aspects under floricultural crops. This review presents an overview of different marker technologies and their variants with a comparative account of their characteristic features and applications especially diverse molecular techniques used in assessing plant genetic diversity, inter-relationship between the genotypes, genetic purity, DNA barcode, tagging of important genes, development of linkage maps, marker assisted selection and application of molecular markers in tissue culture of floricultural crops. Altogether, the characteristics and types of markers with their applications and some recent advancement in genetic studies of floricultural crops are discussed. Highlightsm Molecular markers play an important role for assessing the genetic diversity and identification of desirable traits, inter-relationship between the genotypes and genetic purity analysis. m Molecular markers can be helpful to develop a DNA barcode in flowering crops, tagging of qualitative genes, development of linkage maps, marker assisted selection and clonal fidelity analysis in tissue cultured flowering plants.

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“…The genetically stable Dendrobiums can be propagated by ascertaining the clonal fidelity of the regenerants using different DNA molecular markers. Many investigators have successfully used several DNA markers for genetic homogeneity assessment of different micropropagated Dendrobiums [17,[22][23][24][25]. In this chapter, we highlight the ethnomedicinal uses of Dendrobiums for therapeutic control of ailments and recent works on the in vitro propagation of genetically stable plants using different DNA markers.…”

Section: Introductionmentioning

confidence: 99%

Dendrobium sp.: In vitro Propagation of Genetically Stable Plants and Ethnomedicinal Uses

Tikendra

Apana

Potshangbam

et al. 2022

Reference Series in Phytochemistry

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Plants belonging to the Dendrobium genus occupy a dominant position among the orchids because of their high ornamental and therapeutic values. They are widely popular in the international floriculture trade as they bear stunning flowers with diverse coloration, varied forms, and patterns. The ethnomedicinal uses of these orchids are also prominently found due to the possession of immense medicinal properties. Excessive exploitation through rampant unregulated

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Molecular genetic homogeneity assessment of micropropagated Dendrobium moschatum Sw. - A rare medicinal orchid, using RAPD and ISSR markers

Cited by 40 publications

References 49 publications

Genetic Stability, Phenolic, Flavonoid, Ferulic Acid Contents, and Antioxidant Activity of Micropropagated Lycium schweinfurthii Plants

Genetic Stability, Phenolic, Flavonoid, Ferulic Acid Contents, and Antioxidant Activity of Micropropagated Lycium schweinfurthii Plants

Evaluation of Secondary Metabolites in Wheat Grain (Triticum Sp.) Grown in Humid South Eastern Plain Zone of Rajasthan (India)

Dendrobium sp.: In vitro Propagation of Genetically Stable Plants and Ethnomedicinal Uses

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