Molecular genetic diversity of the pea (Pisum sativum L.) from the Vavilov Research Institute collection detected by the AFLP analysis

Dyachenko, E. A.; Ryzhova, N. N.; Vishnyakova, M. A.; Кочиева, Е. З.

doi:10.1134/s102279541409004x

Cited by 10 publications

(7 citation statements)

References 17 publications

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“…The DNA samples were then subjected to AFLP analysis. Fifteen AFLP primer sets were chosen from the literature [ 30 , 35 ] and tested for polymorphism. Out of them, 12 primer sets exhibited a higher variability and were utilised to study the 25 accessions ( Table 2 ).…”

Section: Methodsmentioning

confidence: 99%

“…Molecular markers are efficient in the evaluation of variation and phylogenetic studies. Amplified fragment length polymorphism (AFLP) proved useful to investigate the diversity levels and relationships in pea germplasm, because of their high degree of polymorphism [ 2 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 ]. Smýkal et al [ 32 ] used microsatellites to evaluate the variability and structure of 164 pea accessions, and reported a considerable level of diversity among accessions.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, Simioniuc et al [ 30 ] used AFLPs to assess the variability and phylogenetic relationships of 21 pea varieties released in Germany. Dyachenko et al [ 35 ] also successfully evaluated the levels of variation among different pea varieties using AFLP markers.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Genetic Variation and Enhancement of Salt Tolerance in French Pea (Pisum Sativum L.)

El‐Esawi

Al-Ghamdi

Ali

et al. 2018

IJMS

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Pisum sativum L. (field pea) is a crop of a high nutritional value and seed oil content. The characterization of pea germplasm is important to improve yield and quality. This study aimed at using fatty acid profiling and amplified fragment length polymorphism (AFLP) markers to evaluate the variation and relationships of 25 accessions of French pea. It also aimed to conduct a marker-trait associations analysis using the crude oil content as the target trait for this analysis, and to investigate whether 5-aminolevulinic acid (ALA) could enhance salt tolerance in the pea germplasm. The percentage of crude oil of the 25 pea genotypes varied from 2.6 to 3.5%, with a mean of 3.04%. Major fatty acids in all of the accessions were linoleic acid. Moreover, the 12 AFLP markers used were polymorphic. The cluster analysis based on fatty acids data or AFLP data divided the 25 pea germplasm into two main clusters. The gene diversity of the AFLP markers varied from 0.21 to 0.58, with a mean of 0.41. Polymorphic information content (PIC) of pea germplasm varied from 0.184 to 0.416 with a mean of 0.321, and their expected heterozygosity (He) varied from 0.212 to 0.477 with a mean of 0.362. The AFLP results revealed that the Nain Ordinaire cultivar has the highest level of genetic variability, whereas Elatius 3 has the lowest level. Three AFLP markers (E-AAC/M-CAA, E-AAC/M-CAC, and E-ACA/M-CAG) were significantly associated with the crude oil content trait. The response of the Nain Ordinaire and Elatius 3 cultivars to high salinity stress was studied. High salinity (150 mM NaCl) slightly reduced the photosynthetic pigments contents in Nain Ordinaire leaves at a non-significant level, however, the pigments contents in the Elatius 3 leaves were significantly reduced by high salinity. Antioxidant enzymes (APX—ascorbate peroxidase; CAT—catalase; and POD—peroxidase) activities were significantly induced in the Nain Ordinaire cultivar, but non-significantly induced in Elatius 3 by high salinity. Priming the salt-stressed Nain Ordinaire and Elatius 3 plants with ALA significantly enhanced the pigments biosynthesis, antioxidant enzymes activities, and stress-related genes expression, as compared to the plants stressed with salt alone. In conclusion, this study is amongst the first investigations that conducted marker-trait associations in pea, and revealed a sort of correlation between the diversity level and salt tolerance.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Analysis of Genetic Variation and Enhancement of Salt Tolerance in French Pea (Pisum Sativum L.)

El‐Esawi

Al-Ghamdi

Ali

et al. 2018

IJMS

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“…Many studies in peas have been conducted on assessment of genetic diversity among genotypes of different regions using several DNA markers, such as restriction fragment length polymorphism (RFLP; Dirlewanger et al, 1994), random amplified polymorphism DNA (RAPD; Thakur et al, 2018 ), simple sequence repeats (SSR; Mohamed et al, 2019;Tahir et al, 2018), sequence-related amplified polymorphism (SRAP; Esposito et al, 2007;Guindon et al, 2016Guindon et al, & 2019, inter-simple sequence repeat (ISSR; La´zaro and Aguinagalde, 2006;Pakseresht et al, 2013) and amplified fragment length polymorphism (AFLP; Dyachenko et al, 2014). Among them, SRAP and ISSR have gained popularity because they are simple, speed, effective, reproducible, and do not need prior information about the target sequences in the genome (Snowdon and Friedt, 2004).…”

Section: Introductionmentioning

confidence: 99%

Genetic Relationships and Diversity among Pea (Pisum sativum L.) Genotypes Assessed using Agro-Morphological and Molecular Markers

El-Fatah

Nafea

2020

Journal of Agricultural Chemistry and Biotechnology

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Fifteen pea (Pisum sativum L.) genotypes were evaluated for their agro-morphological performance and molecular diversity during 2017-2018 and 2018-2019 seasons. Combined analysis of variance revealed that there are highly significant differences among pea genotypes tested for all agro-morphological traits studied. The phenotypic coefficients of variation (PCV) values were relatively greater than Genotypic coefficients of variation (GCV) for all traits; however, GCV values were near to PCV values for the traits, like plant height, pods/plant, 100 seeds weight and pod width. High heritability coupled with high genetic advance was obtained for, plant height, reflecting the presence of additive gene action for the expression of this trait. High to moderate heritability coupled with moderate genetic gain were exhibited for number of pods per plant, 100 seeds weight, pod length, seeds per pod, pod weight and number of branches per plant. The Euclidean Distance among all genotypes based on agro-morphological traits was relatively wide. ISSR and SRAP markers were used to study the molecular diversity among pea genotypes. The molecular marker parameters revealed that SRAP markers were more efficient than ISSR markers with regards to polymorphism detection and in distinction among pea genotypes. Also, both ISSR and SRAP markers were able to amplify unique bands specific to a particular genotype. A positive and significant correlation (r=0.389: p ≤ 0.001) between ISSR and SRAP matrix was observed according to Mantel's test. Also, significant correlation (r = 0.411: p ≤ 0.001) between the matrices of combined molecular markers data and agro-morphological data.

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“…Apart from P. sativum (field pea) this genus includes only P. fulvum (tawny pea), which is a valuable source of resistance genes to various phy topathogens [8][9][10].…”

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confidence: 99%

Identification and polymorphism of the sucrose synthase gene Sus1 in Pisum fulvum

2015

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Molecular genetic diversity of the pea (Pisum sativum L.) from the Vavilov Research Institute collection detected by the AFLP analysis

Cited by 10 publications

References 17 publications

Analysis of Genetic Variation and Enhancement of Salt Tolerance in French Pea (Pisum Sativum L.)

Analysis of Genetic Variation and Enhancement of Salt Tolerance in French Pea (Pisum Sativum L.)

Genetic Relationships and Diversity among Pea (Pisum sativum L.) Genotypes Assessed using Agro-Morphological and Molecular Markers

Identification and polymorphism of the sucrose synthase gene Sus1 in Pisum fulvum

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