Molecular Genetic Dissection of Spawning, Parentage, and Reproductive Tactics in a Population of Redbreast Sunfish, Lepomis auritus

DeWoody, J. Andrew; Fletcher, Dean E.; Wilkins, Selwyn; Nelson, W. John; Avise, John C.

doi:10.2307/2411350

Cited by 39 publications

(33 citation statements)

References 19 publications

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“…We did not find specimens of C. virginicus in areas with finer substrata associated with lower flows along the periphery of the stream. Although the one nest of Lepomis auritus sampled was constructed of gravel, it was located along the periphery of the stream, surrounded by finer substrata, and did not contain specimens of C. virginicus [nor did any of the 25 nests of L. auritus surveyed from Fourmile Creek in the Savannah River system by DeWoody et al (1998); (J.A. DeWoody, pers.…”

Section: Discussionmentioning

confidence: 99%

Egg‐feeding in the freshwater piscicolid leech Cystobranchus virginicus (Annelida, Hirudinea)

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Fiumera

Porter

2005

Invertebrate Biology

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Abstract. Leeches are common terrestrial and aquatic annelids, parasitizing or feeding on a wide range of host taxa. Here we report evidence for an unusual feeding behavior of egg‐feeding in the piscicolid leech Cystobranchus virginicus. We identified distended specimens of C. virginicus in the nests of at least 4 different fish species: Campostoma anomalum, Moxostoma carinatum, Moxostoma sp. (either M. anisurum and/or M. breviceps), and Nocomis leptocephalus. We collected a total of 41 leeches from the nests of these host species and documented at least 1 leech in 19 of 55 nests (35%), with many sites containing multiple leeches. Individuals of C. virginicus were not identified feeding on any of the 41 adult specimens of Moxostoma spp. or the 635 adult specimens of Nocomis leptocephalus examined, and were never found in the absence of active host spawning (26 sites). These results are consistent with individuals of C. virginicus being an opportunistic or possibly even an obligate egg‐feeder, potentially timing their own reproductive activities with the spawning of their fish hosts. The current distribution of C. virginicus has been expanded to include North Carolina, South Carolina, and Tennessee. The potential for leech species to induce mortality in developing fish eggs could be a concern for fish conservation and merits further investigation.

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Section: Discussionmentioning

confidence: 99%

Egg‐feeding in the freshwater piscicolid leech Cystobranchus virginicus (Annelida, Hirudinea)

Light

Fiumera

Porter

2005

Invertebrate Biology

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“…DNA was later isolated from the adults and a subset of larvae, and microsatellite DNA fingerprints were obtained by using multiplex genotyping (Colbourne et al, 1996;Neff et al, 2000). Up to four primer sets (locus: RB7, RB20, LMAR10, LMAR14; published in DeWoody et al, 1998 andSchable et al, 2002) were simultaneously amplified, and all competing parental and cuckolder offspring were unambiguously identified by using exclusion methods (Chakraborty et al, 1988).…”

Section: Sperm Competitivenessmentioning

confidence: 99%

Sperm competition in a fish with external fertilization: the contribution of sperm number, speed and length

Stoltz

Neff

2006

Journal of Evolutionary Biology

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The role of sperm number and quality in male competitiveness was investigated using in vitro fertilization experiments with bluegill (Lepomis macrochirus). Bluegill males use one of three mating tactics: ‘sneakers’, which streak spawn; ‘satellites’, which mimic females; and ‘parentals’, which are territorial. The in vitro experiments mimicked natural spawning by incorporating these males’ mean proximity to eggs and timing of sperm release. Using a maximum‐likelihood algorithm, raffle equations were fit to paternity data, which revealed a strong effect of sperm number on male competitiveness. There was no difference in sperm flagellum length, curvilinear swim speed or path linearity among the three male mating types, and these traits did not explain any additional variation in male competitiveness. It was estimated that, given closer proximity to eggs, satellites need release only 0.34 times as many sperm as parentals to obtain equal paternity. Despite being farther from the eggs and releasing sperm about half a second after parentals, sneakers need only release 0.58 times as many sperm as parentals to obtain equal paternity. Thus, the increased competitiveness of sneakers’ sperm must come from a component of sperm quality other than speed or length.

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“…Each individual was genotyped at three microsatellite loci: RB7, LMAR10 and either RB20 or LMAR14 (for details of these loci see [9,10]). RB20 was used for the parentage analysis of the 2005 bluegill samples, whereas LMAR14 was used for the interspecies allele frequency comparisons and the parentage analysis of the 2003 and 2010 pumpkinseed samples.…”

Section: (B) Genetic Analysismentioning

confidence: 99%

Alternative male reproductive tactics drive asymmetrical hybridization between sunfishes ( Lepomis spp.)

Garner

Neff

2013

Biol. Lett.

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The potential role of alternative reproductive tactics in circumventing premating isolating mechanisms and driving hybridization between species has long been recognized, but to date there is little empirical support from natural systems. Hybridization occurs between bluegill (Lepomis macrochirus) and pumpkinseed sunfish (Lepomis gibbosus) and it is known to be asymmetrical (male bluegill Â female pumpkinseed). Here, we test whether this pattern is driven by a recognition failure by pumpkinseed females or by an alternative cuckolder reproductive tactic in bluegill males. Using genetic parentage data, we found that bluegill cuckolders fathered 24.9% of the larvae in bluegill nests, but no evidence that pumpkinseed females spawned in bluegill nests. Pumpkinseed cuckolders fathered 8.7% of the larvae in pumpkinseed nests, whereas bluegill cuckolders fathered 13.6% of the larvae in those nests. Bluegill cuckolders thus frequently spawn in pumpkinseed nests and are responsible for the asymmetrical hybridization between the species. We discuss the evolutionary consequences of interactions between bluegill and pumpkinseed and the role of alternative reproductive tactics in adaptation and introgression.

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Molecular Genetic Dissection of Spawning, Parentage, and Reproductive Tactics in a Population of Redbreast Sunfish, Lepomis auritus

Cited by 39 publications

References 19 publications

Egg‐feeding in the freshwater piscicolid leech Cystobranchus virginicus (Annelida, Hirudinea)

Egg‐feeding in the freshwater piscicolid leech Cystobranchus virginicus (Annelida, Hirudinea)

Sperm competition in a fish with external fertilization: the contribution of sperm number, speed and length

Alternative male reproductive tactics drive asymmetrical hybridization between sunfishes ( Lepomis spp.)

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