Molecular genetic characterization of avian Chlamydophila psittaci isolates

Yatsentyuk, Svetlana P.; Обухов, И Л

doi:10.1134/s1022795407110026

Cited by 5 publications

(10 citation statements)

References 14 publications

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“…While representatives of avian C . abortus were rarely encountered in previous studies [10–11, 16, 41], the present study shows for the first time that avian genotypes of C . abortus strains are actively circulating in wild bird populations.…”

Section: Discussionsupporting

confidence: 61%

“…psittaci genotypes Mat116 (black-headed gull) and 1V (Eurasian magpie and hooded crow). These genotypes, so far, were only represented by single isolates [6, 10]. However, the majority of samples from this study was not assigned to any of the established genotypes, but formed two separate branches designated G1 and G2 in the omp A sequence-based dendrogram ( Fig 2 ).…”

Section: Discussionmentioning

confidence: 82%

“…Likewise, G1was detected in swans, Eurasian teals and coots at six separate locations in the country whereas genotype 1V was identified in magpies and crows at only one site. However, considering also the evidence for the presence of G1 and G2 in mallards from Sweden [16] and of 1V in crows from Russia [10], an at least Europe-wide distribution of these avian C . abortus strains in wildfowl can be assumed.…”

Section: Discussionmentioning

confidence: 99%

“…psittaci has been classified into fifteen genotypes, each one more or less closely associated with certain bird species. Seven of these genotypes (A-F, E/B) are predominant whereas the other eight genotypes (1V, 6N, Mat116, R54, YP84, CPX0308, I and J) were described as provisional [6–10]. Whereas C .…”

Section: Introductionmentioning

confidence: 99%

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Dissemination and genetic diversity of chlamydial agents in Polish wildfowl: Isolation and molecular characterisation of avian Chlamydia abortus strains

et al. 2017

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Wild birds are considered as a reservoir for avian chlamydiosis posing a potential infectious threat to domestic poultry and humans. Analysis of 894 cloacal or fecal swabs from free-living birds in Poland revealed an overall Chlamydiaceae prevalence of 14.8% (n = 132) with the highest prevalence noted in Anatidae (19.7%) and Corvidae (13.4%). Further testing conducted with species-specific real-time PCR showed that 65 samples (49.2%) were positive for C. psittaci whereas only one was positive for C. avium. To classify the non-identified chlamydial agents and to genotype the C. psittaci and C. avium-positive samples, specimens were subjected to ompA-PCR and sequencing (n = 83). The ompA-based NJ dendrogram revealed that only 23 out of 83 sequences were assigned to C. psittaci, in particular to four clades representing the previously described C. psittaci genotypes B, C, Mat116 and 1V. Whereas the 59 remaining sequences were assigned to two new clades named G1 and G2, each one including sequences recently obtained from chlamydiae detected in Swedish wetland birds. G1 (18 samples from Anatidae and Rallidae) grouped closely together with genotype 1V and in relative proximity to several C. abortus isolates, and G2 (41 samples from Anatidae and Corvidae) grouped closely to C. psittaci strains of the classical ABE cluster, Matt116 and M56. Finally, deep molecular analysis of four representative isolates of genotypes 1V, G1 and G2 based on 16S rRNA, IGS and partial 23S rRNA sequences as well as MLST clearly classify these isolates within the C. abortus species. Consequently, we propose an expansion of the C. abortus species to include not only the classical isolates of mammalian origin, but also avian isolates so far referred to as atypical C. psittaci or C. psittaci/C. abortus intermediates.

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Section: Discussionsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dissemination and genetic diversity of chlamydial agents in Polish wildfowl: Isolation and molecular characterisation of avian Chlamydia abortus strains

et al. 2017

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“…Although studies involving the genetic characterization of Chlamydia spp. are prevalent on a global scale [ 8 , 17 , 23 , 30 ], there is little information on the occurrence or epidemiology of Chlamydia spp. infection in wild birds of South Korea.…”

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confidence: 99%

Molecular prevalence and genotyping of <i>Chlamydia</i> spp. in wild birds from South Korea

Jeong

et al. 2017

The Journal of Veterinary Medical Science

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Wild birds are reservoirs for Chlamydia spp. Of the total 225 samples from wild birds during January to September 2016 in Korea, 4 (1.8%) and 2 (0.9%) showed positive for Chlamydia psittaci and Chlamydia gallinacea, respectively. Phylogenetic analyses and comparisons of sequence identities for outer-membrane protein A (ompA) revealed that Korean C. psittaci fall into three previously known genotypes; genotype E, 1V and 6N, whereas the Korean C. gallinacea were classified as new variants of C. gallinacea. Our study demonstrates that wild birds in South Korea carry at least two Chlamydia species: C. psittaci and C. gallinacea, and provides new information on the epidemiology of avian chlamydiosis in wild birds.

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Emerging and well‐characterized chlamydial infections detected in a wide range of wild Australian birds

Kasimov

Dong

Shao

et al. 2022

Transbounding Emerging Dis

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Birds can act as successful long‐distance vectors and reservoirs for numerous zoonotic bacterial, parasitic and viral pathogens, which can be a concern given the interconnectedness of animal, human and environmental health. Examples of such avian pathogens are members of the genus Chlamydia. Presently, there is a lack of research investigating chlamydial infections in Australian wild and captive birds and the subsequent risks to humans and other animals. In our current study, we investigated the prevalence and genetic diversity of chlamydial organisms infecting wild birds from Queensland and the rate of co‐infections with beak and feather disease virus (BFDV). We screened 1114 samples collected from 564 different birds from 16 orders admitted to the Australia Zoo Wildlife Hospital from May 2019 to February 2021 for Chlamydia and BFDV. Utilizing species‐specific quantitative polymerase chain reaction (qPCR) assays, we revealed an overall Chlamydiaceae prevalence of 29.26% (165/564; 95% confidence interval (CI) 25.65–33.14), including 3.19% (18/564; 95% CI 2.03–4.99%) prevalence of the zoonotic Chlamydia psittaci. Chlamydiaceae co‐infection with BFDV was detected in 9.75% (55/564; 95% CI 7.57–12.48%) of the birds. Molecular characterization of the chlamydial 16S rRNA and ompA genes identified C. psittaci, in addition to novel and other genetically diverse Chlamydia species: avian Chlamydia abortus, Ca. Chlamydia ibidis and Chlamydia pneumoniae, all detected for the first time in Australia within a novel avian host range (crows, figbirds, herons, kookaburras, lapwings and shearwaters). This study shows that C. psittaci and other emerging Chlamydia species are prevalent in a wider range of avian hosts than previously anticipated, potentially increasing the risk of spill‐over to Australian wildlife, livestock and humans. Going forward, we need to further characterize C. psittaci and other emerging Chlamydia species to determine their exact genetic identity, potential reservoirs, and factors influencing infection spill‐over.

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Molecular genetic characterization of avian Chlamydophila psittaci isolates

Cited by 5 publications

References 14 publications

Dissemination and genetic diversity of chlamydial agents in Polish wildfowl: Isolation and molecular characterisation of avian Chlamydia abortus strains

Dissemination and genetic diversity of chlamydial agents in Polish wildfowl: Isolation and molecular characterisation of avian Chlamydia abortus strains

Molecular prevalence and genotyping of <i>Chlamydia</i> spp. in wild birds from South Korea

Emerging and well‐characterized chlamydial infections detected in a wide range of wild Australian birds

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