Molecular genetic characteristics of Saccharomyces cerevisiae distillers’ yeasts

Наумова, Е. С.; Sadykova, A. Zh.; Мартыненко, Н. Н.; Наумов, Г. И.

doi:10.1134/s0026261713020112

Cited by 14 publications

(20 citation statements)

References 31 publications

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“…Aneuploidy has also been found in the yeasts used for the distilling of spirits such and vodka and whisky (Adamczyk et al, 2016;Deregowska et al, 2015;Naumova, Sadykova, Martynenko, & Naumov, 2013). Aneuploidies in distilling strains have been linked with increased copy number of MAL and SUC genes, which are known to play important roles in the distilling process (Naumova et al, 2013).…”

Section: Aneuploidy In Industrial Strainsmentioning

confidence: 99%

Aneuploidy in yeast: Segregation error or adaptation mechanism?

Gilchrist

Stelkens

2019

Yeast

104

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Aneuploidy is the loss or gain of chromosomes within a genome. It is often detrimental and has been associated with cell death and genetic disorders. However, aneuploidy can also be beneficial and provide a quick solution through changes in gene dosage when cells face environmental stress. Here, we review the prevalence of aneuploidy in Saccharomyces, Candida, and Cryptococcus yeasts (and their hybrid offspring) and analyse associations with chromosome size and specific stressors. We discuss how aneuploidy, a segregation error, may in fact provide a natural route for the diversification of microbes and enable important evolutionary innovations given the right ecological circumstances, such as the colonisation of new environments or the transition from commensal to pathogenic lifestyle. We also draw attention to a largely unstudied cross link between hybridisation and aneuploidy. Hybrid meiosis, involving two divergent genomes, can lead to drastically increased rates of aneuploidy in the offspring due to antirecombination and chromosomal missegregation. Because hybridisation and aneuploidy have both been shown to increase with environmental stress, we believe it important and timely to start exploring the evolutionary significance of their co‐occurrence.

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Section: Aneuploidy In Industrial Strainsmentioning

confidence: 99%

Aneuploidy in yeast: Segregation error or adaptation mechanism?

Gilchrist

Stelkens

2019

Yeast

104

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“…It is known that the polymeriс genes of each family are responsible for the same function (α-glucosidase or β-fructosidase), but they have different chromosomal subtelomeric localization (Carlson & Botstein, 1983;Carlson, Celenza, & Eng, 1985;Naumov & Naumova, 2010a, 2010bNaumov, Naumova, & Michels, 1994). The presence of several MAL or SUC genes in the genome of the same strain has a cumulative effect leading to an intensification of maltose or sucrose fermentations (Haupt, Alps, & Mugele, 1963;Hohmann, 1987;Naumova, Sadykova, Martynenko, & Naumov, 2013;Oda & Ouchi, 1989b The presence of RTM1 gene was demonstrated in the ALKO strains by restriction mapping, and the chromosomal locations are shown for ALKO 743 in Figure 5, Lane 10. All the five RTM1hybridizing bands coincide with the subtelomeric SUC genes except with SUC2, which is not located in the subtelomeric region and is known to lack the association of RTM1 (Denayrolles, Pinot de Villechenon, Lonvaad-Funel, & Aigle, 1997).…”

Section: Heterozygosity Of Alko 743 and Alko 3460mentioning

confidence: 99%

Exploiting heterozygosity in industrial yeasts to create new and improved baker's yeasts

Korhola

Наумова

Partti

et al. 2019

Yeast

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The main aim of the work was to utilize heterozygosity of industrial yeast strains to construct new baker's yeast strains. Commercial baker's yeast strain ALKO 743, its more ethanol tolerant descendant ALKO 554 selected initially for growth over 300 generations in increasing ethanol concentrations in a glucose medium, and ALKO 3460 from an old domestic sour dough starter were used as starting strains. Isolated meiotic segregants of the strains were characterized genetically for sporulation ability and mating type, and the ploidy was determined physically. Heterozygosity of the segregant strains was estimated by a variety of molecular characterizations and fermentation and growth assays. The results showed wide heterozygosity and that the segregants were clustered into subgroups. This clustering was used for choosing distantly or closely related partners for strain construction crosses. Intrastrain hybrids made with segregants of ALKO 743 showed 16–24% hybrid vigour or heterosis. Interstrain hybrids with segregants of ALKO 743 and ALKO 3460 showed a wide variety of characteristics but also clear heterosis of 27–31% effects as assayed by lean and sugar dough raising. Distiller's yeast ALKO 554 turned out to be a diploid genetic segregant and not just a more ethanol tolerant mutant of the tetraploid parent strain ALKO 743.

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“…Cultured S. cerevisiae strains, in contrast to envi ronmental isolates, contain different sets of SUC genes [20][21][22]. No special SUC genes with inulinase activity have been discovered.…”

Section: Polymeric β Fructosidase Genes Suc and Regulation Of Their Ementioning

confidence: 99%

“…The XII race, which was obtained from the All Russian Collection of Microorganisms (VKM) in Pushchino, Russia, presumably under the number VKM Y 1169 was used as a starting strain. Earlier [22], we used molecular karyotyping and sub sequent Southern hybridization of the SUC2 probe with chromosomal DNA to determine the genotype of the strain as SUC1 SUC2 SUC7 SUC8. Importantly, distiller's strains in general have many SUC genes [21,22].…”

Section: S Cerevisiae Mutant Can Utilizementioning

confidence: 99%

“…Earlier [22], we used molecular karyotyping and sub sequent Southern hybridization of the SUC2 probe with chromosomal DNA to determine the genotype of the strain as SUC1 SUC2 SUC7 SUC8. Importantly, distiller's strains in general have many SUC genes [21,22]. The genotype of the XII race makes it possible to expect its high invertase activity, which, obviously, can become still further in the regulatory gene mutants.…”

Section: S Cerevisiae Mutant Can Utilizementioning

confidence: 99%

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