Molecular genetic analysis of weak B blood group allele in Jinan population reveals distribution of ABw phenotype

Abstract

The aim of this study was to investigate the distribution of the ABw phenotype of ABO blood group in the Jinan population. 31 856 samples were tested during the period 2018 to 2019. Thirty-nine samples with discrepant results, as identified by micro-column gel method, were further investigated by serological (tube technique) and molecular (fluorescence PCR, DNA sequencing) methods. Eight samples showed ABw phenotype, which accounted for 0.025% of the population tested. From the sequencing analysis, six samples (6/8) were typed as ABO*A1.02/ABO*BW.12 and two samples (2/8) as ABO*A1.02/ABO*BW.03. The study suggests that ABw12 account for 75% of ABw phenotype and indicate ABw12 is the main ABw phenotype in Jinan population.