Molecular Genetic Analysis of Reovirus Isolated in Korea.

Abstract

Summary: Reovirus isolates from human, striped-field mouse (Apodemus agrarius) and Korean field mouse (A. peninsulae) in Korea showed extensive variability in the patterns of electrophoretic migration of the double-stranded RNA (dsRNA) genome segments. Hemagglutination inhibition (HI) test was performed for serotype determination of 12 reovirus isolates. To clarify genetic diversity and molecular phylogeny of Korean reoviruses, L1, S3 and S4 genomic segments of reoviruses were amplified by RT-PCR and directly sequenced. Among 12 reovirus strains, 9 strains were type 3 and 3 strains were type 2. The L1 was highly conserved showing 91.5-100%, 94.7-100% similar ities among Korean isolates, and 77.5-97.9%, 92.6-96.8% similarities compared to other reference strains of each genotypes at nucleotide and amino acid levels, respectively. In S3 and S4 segments, 84.4-99.3%, 72.3-99% nucleotide sequence similarities and 92-99.3%, 89.1-98.4% amino acid sequence similarities among Korean isolates were observed, and 70.8-93.9%, 72.3-98.7% nucleotide sequence similarities and 81.8-100%, 88.3-97.7% amino acid sequence similarities compared with other reference strains of each genotype were observed, respectively. Phylogenetic analyses based on the S3 and S4 nucleotide sequences indicate that genotypes of reovirus are more related with geographic differences rather than host species or date of isolation.

Detection of Enteroviruses and Mammalian Reoviruses in Korean Environmental Waters

“…The denaturation, annealing, and extension conditions were 95 C for 1 min, 45 C for 1 min and 72 C for 2 min, respectively, followed by a final incubation for 10 min at 72 C. For the partial amplification of L1 segment of reoviruses, cDNA was amplified using two sets of primer pairs (RV1 5'-CCATTTATGGGGGTTCCTGC-3'/ RV2 5'-YTTTAARTAYTCKGCRGTSCC-3') (11). The second PCR was performed using other two sets of primer pairs (RV3 5'-CAYATGACYACSACTTTCC-CK-3'/ RV4 5'-GCKATGTCATATTTCCATCCG-3') (11). The denaturation, annealing, and extension conditions were 95 C for 1 min, 55 C for 1 min and 72 C for 2 min, respectively, followed by a final incubation for 10 min at 72 C. Quality controls.…”

“…It is important to detect and be aware of the pathogenesis of reoviruses in water samples as these viruses have been closely associated with minor respiratory and gastrointestinal illnesses of infants and young children (8,11). Our data also supported that the detection of reoviruses could be used to evaluate viral contamination in surface water, finished water, and tap water and showed that reoviruses could possibly be used as an indicator of fecal and viral contamination of environmental waters along with the commonly monitored enteroviruses.…”

“…It was reversely transcribed into enterovirus and reovirus cDNA, respectively. Enterovirus, reovirus, HAV, and adenovirus-specific primers were prepared based on the sequences reported previously (7,11,22). cDNA was amplified using each set of primer pairs for enterovirus (Et1 (ϩ) 5'-CCTCCGGCCCCTGAATG-3'/ Et2 (Ϫ) 5'-ACCGGATGGCCAATCCAA-3'), HAV (HAV1 (ϩ) 5'-CAGCACATCAGAAAGGTGAG-3'/ HAV2 (Ϫ) 5'-CTCCAGAATCATCTCCAA-3'), and adenovirus (A1 (ϩ) 5'-GCCCCAGTGGTCTTACATGCACATC-3'/ A2 (Ϫ) 5'-CAGCACGCCGCGGATGTCAAAGT-3') in DNA thermal cycler 480 (Perkin Elmer Cetus, Norwalk, Conn., U.S.A.) for 33 cycles.…”

Design and Performance Evaluation of a Scalable Authentication Protocol in Mobile IP

“…The proposed algorithm may reduce the computational burden of the complicated public key algorithm because the public key cryptography and certificate verification are applied only at the first authentication step, and symmetric key algorithm is used thereafter. Recently, due to improvement of hardware, public key computation in a mobile node is becoming more feasible [10], [11].…”

Characterization of replication and variations in genome segments of a bat reovirus, BatMRV/B19-02, by RNA-seq in infected Vero-E6 cells

“…To date, four reovirus serotypes have been identified: MRV1, MRV2, MRV3, and the novel serotype MRV4, for which only one strain (Ndelle virus) has been identified [5]. Recently, reovirus strains belonging to serotypes 2 and 3 were isolated from humans, mice, and swine in South Korea [12,15]; however, MRV1 has not been identified in mammals in South Korea.…”