Molecular genetic analysis of genomes of porcine respiratory and reproductive syndrome viruses and porcine circovirus type 2 circulating on the territory of the Russian federation

Bulgakov, A.D.; Гребенникова, Т. В.; Yuzhakov, Anton G.; Алипер, Т.И.; Nepoklonov, E A

doi:10.3103/s0891416814040028

Cited by 3 publications

(2 citation statements)

References 8 publications

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“…This data corroborated previous findings that PCV2d is currently the predominant genotype of PCV2 in most countries where pork production is well-developed [13,14,20]. Remarkably, our data suggested that in the Siberian and Far Eastern districts, which border on the People's Republic of China, PCV2a and PCV2b have been completely replaced by PCV2d [21].…”

Section: Pcv-2d Is the Predominant Genotype In Russiasupporting

confidence: 92%

Whole-Genome Analysis of Porcine Circovirus Type 2 in Russia

2021

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Porcine circovirus type 2 (PCV2) is the causative agent of porcine circovirus-associated diseases (PCVAD) that bring about significant economic losses in the pig industry all over the world. The aim of this study was to investigate the genetic diversity of PCV2 in Russia and characterize the available complete genome sequences. PCV2 DNA was detected at all investigated farms located in different regions of Russia. Whole-genome analysis demonstrated that the majority of PCV2 strains belonged to genotype PCV2d (12 out of 14), while PCV2a and PCV2b were only detected at 2 farms (one at each). Further analysis revealed that all antibody recognition sites in Russian PCV2 strains were different from the corresponding epitopes in a PCV2a vaccine strain, suggesting that PCV2a-based vaccines may only provide limited protection against these strains. PCV2d strains could be grouped into 3 distinct lines which shared 98.7–100% identity within open reading frame 2 (ORF2). It is the first study reporting the genetic diversity of PCV2 strains in Russia. Our data indicated that, similarly to China, Europe, and USA, PCV2a and PCV2b have largely been replaced by PCV2d.

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Section: Pcv-2d Is the Predominant Genotype In Russiasupporting

confidence: 92%

Whole-Genome Analysis of Porcine Circovirus Type 2 in Russia

2021

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“…Цирковирусные болезни у свиней могут проявляться в виде субклинической и клинической инфекций, при этом субинфекция является наиболее распространенной формой инфекционного процесса, вызываемого ЦВС-2, и выражается в потере среднесуточного прироста массы тела без специфических клинических признаков [2]. Системное заболевание поражает поросят преимущественно в возрасте 6-15 недель и характеризуется анорексией, диспептическими явлениями и лимфаденопатией [3]. Случаи массовой заболеваемости свиней цирковирусными инфекциями зарегистрированы в большинстве регионов мира, что влечет за собой серьезные экономические последствия для свиноводческой отрасли [4,5,6,7].…”

Section: Introductionunclassified

Construction of prokaryotic system for expression of porcine circovirus type 2 ORF-2 gene fragment

Galeeva,

Akhunova,

Usoltsev

et al. 2024

Veterinariâ segodnâ

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Porcine circovirus-associated diseases (PCVDs) are among the most significant challenges for pig farming in developed countries. Porcine circovirus type 2 (PCV-2) is considered the main etiological agent of postweaning multisystemic wasting syndrome in piglets. Mass PCVD occurrence has been reported in most regions of the world, that results in serious economic consequences. Optimal PCVD prevention is known to be achieved through a set of veterinary and sanitary measures in combination with vaccination. High evolutionary virus variability facilitating new genotype and strain emergence requires development of new candidate recombinant vaccines against PCV-2 infection. The study was aimed at construction of prokaryotic system for PCV-2 ORF-2 gene fragment expression and its functionality assessment. A genetic insert constructed from the most immunogenic type-specific PCV-2 epitopes based on genotype 2a, 2b, 2d strain and isolate consensus sequence was cloned into the expression vector pET-22b(+) that was incorporated into the Escherichia coli strain Rosetta 2(DE3). The transformants were selected based on the marker gene of ampicillin resistance on a selective medium. Target gene expression was induced by adding of isopropyl-β-D-1-thiogalactopyranoside at different concentrations. As a result, Escherichia coli Rosetta 2(DE3)/pET-22b-ORF-2 strain, a producer of capsid protein fragment (92–233 amino acid residues), was constructed. It was found that in the presence of 1 mM isopropyl-β-D-1-thiogalactopyranoside, the expression level of soluble truncated rCap was 35–40 mg/L 6 hours after induction. The expression product was tested for its specificity with indirect ELISA using whole-virion PCV-2-hyperimmunized porcine serum. It was shown that the positivity coefficient of producer strain cell lysates averaged to 4.34 (p < 0.005). The recombinant rCap protein is suitable for serological diagnosis and is also of interest as a vaccine component, which is the goal of our further studies.

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Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): porcine reproductive and respiratory syndrome (PRRS)

More¹,

Bøtner²,

Butterworth³

et al. 2017

EFS2

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Porcine reproductive and respiratory syndrome (PRRS) has been assessed according to the criteria of the Animal Health Law (AHL), in particular criteria of Article 7 on disease profile and impacts, Article 5 on the eligibility of PRRS to be listed, Article 9 for the categorisation of PRRS according to disease prevention and control rules as in Annex IV and Article 8 on the list of animal species related to PRRS. The assessment has been performed following a methodology composed of information collection and compilation, expert judgement on each criterion at individual and, if no consensus was reached before, also at collective level. The output is composed of the categorical answer, and for the questions where no consensus was reached, the different supporting views are reported. Details on the methodology used for this assessment are explained in a separate opinion. According to the assessment performed, PRRS can be considered eligible to be listed for Union intervention as laid down in Article 5(3) of the AHL. The disease would comply with the criteria as in Sections 4 and 5 of Annex IV of the AHL, for the application of the disease prevention and control rules referred to in points (d) and (e) of Article 9(1). The animal species to be listed for PRRS according to Article 8(3) criteria are domestic pigs and wild boar.

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Molecular genetic analysis of genomes of porcine respiratory and reproductive syndrome viruses and porcine circovirus type 2 circulating on the territory of the Russian federation

Cited by 3 publications

References 8 publications

Whole-Genome Analysis of Porcine Circovirus Type 2 in Russia

Whole-Genome Analysis of Porcine Circovirus Type 2 in Russia

Construction of prokaryotic system for expression of porcine circovirus type 2 ORF-2 gene fragment

Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): porcine reproductive and respiratory syndrome (PRRS)

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