Molecular Function Predictions and Diagnostic Value Analysis of Plasma Exosomal miRNAs in Hirschsprung’s Disease

Lv, Xiurui; Li, Yuhan; Li, Hongxing; Zhou, Lingling; Wang, Binyu; Zhi, Zhengke; Tang, Weibing

doi:10.2217/epi-2019-0190

Cited by 11 publications

(24 citation statements)

References 41 publications

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“…Due to the low abundance of small noncoding RNAs in exosomes, the number of DE miRNAs based on adjusted P values was too low to perform any further analysis. In fact, thresholds in high-throughput sequencing may be set at unadjusted P values to determine the DE miRNAs, which has been reported previously [ 22 ]. If the expression level was too low to detect (displayed as zero), the expression level was set as 0.001 in order to calculate the fold change.…”

Section: Methodsmentioning

confidence: 99%

Plasma-Derived Exosomal hsa-miR-4488 and hsa-miR-1228-5p: Novel Biomarkers for Dermatomyositis-Associated Interstitial Lung Disease with Anti-Melanoma Differentiation-Associated Protein 5 Antibody-Positive Subset

Zhong

et al. 2021

BioMed Research International

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The present study is aimed at profiling circulating exosome-derived microRNAs (miRNAs/miRs) from patients with dermatomyositis (DM), in particular those complicated with interstitial lung disease (ILD) with anti-melanoma differentiation-associated protein 5 (MDA5) antibody-positive. Fifteen participants were enrolled, including five patients with DM complicated with ILDs prior to treatment with circulating anti-MDA5 antibody-positive status [DM-ILD-MDA5 Ab(+)], five DM patients without ILDs who were negative for 16 detectable myositis-specific antibodies [DM-nonILD-MSA16(-)], and five age- and gender-matched healthy donor controls (HCs). The characteristics of the exosomes extracted by Ribo™ Exosome Isolation Reagent were identified using transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), and flow cytometry. Differentially expressed miRNAs, determined by next-generation deep sequencing, were identified through the criteria of ∣ log 2 fold change ∣ ≥ 1 and P < 0.01 . A total of 38 miRNAs were significantly upregulated in exosomes from patients with DM-ILD-MDA5 Ab(+) compared to those from HC, while 21 miRNAs were significantly downregulated. Compared to exosomes derived from patients with DM-nonILD-MSA16(-), 51 miRNAs were significantly upregulated and 33 miRNAs were significantly downregulated from patients with DM-ILD-MDA5 Ab(+). A total of 73 exosomal miRNAs were significantly differentially expressed between DM-nonILD-MSA16(-) and HC. In particular, two miRNAs, Homo sapiens- (hsa-) miR-4488 and hsa-miR-1228-5p, were common differentially expressed miRNAs among three comparisons. GO and KEGG analyses suggested that several pathways may contribute the pathogenesis of DM-ILD-MDA5 Ab(+) and DM-nonILD-MSA16(-), while PPI network analysis of hsa-miR-4488 and hsa-miR-1228-5p indicated that their predicted target genes, DExD-box helicase 39B and MDM2, may be involved in the mechanisms of DM-ILD-MDA5 Ab(+).

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Section: Methodsmentioning

confidence: 99%

Plasma-Derived Exosomal hsa-miR-4488 and hsa-miR-1228-5p: Novel Biomarkers for Dermatomyositis-Associated Interstitial Lung Disease with Anti-Melanoma Differentiation-Associated Protein 5 Antibody-Positive Subset

Zhong

et al. 2021

BioMed Research International

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“…further found that MSC-derived exosomal miR-100 provides protection to the articular cartilage and helps in regulation of cartilage homeostasis in the OA mice model via inhibition of mTOR-autophagy pathway [149]. Further, it has been identified that plasma-derived exosomal miRNAs are involved in 'extracellular matrix-receptor interaction' and contribute to Hirschsprung's disease through interfering in cell junctions [166]. Human adipose stem cell-derived exosomes loaded with miR-21 mimics play a critical role in cell proliferation and migration of keratinocytes, and treatment of diabetic chronic wounds with miR-21 mimics results in accelerated healing by collagen remodeling, increasing re-epithelization, vessel maturation, and angiogenesis in vivo [167].…”

Section: Rnasmentioning

confidence: 97%

Mesenchymal Stem Cell-Derived Exosomes: Applications in Regenerative Medicine

Hade

Suire

Suo

2021

Cells

203

154

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Exosomes are a type of extracellular vesicles, produced within multivesicular bodies, that are then released into the extracellular space through a merging of the multivesicular body with the plasma membrane. These vesicles are secreted by almost all cell types to aid in a vast array of cellular functions, including intercellular communication, cell differentiation and proliferation, angiogenesis, stress response, and immune signaling. This ability to contribute to several distinct processes is due to the complexity of exosomes, as they carry a multitude of signaling moieties, including proteins, lipids, cell surface receptors, enzymes, cytokines, transcription factors, and nucleic acids. The favorable biological properties of exosomes including biocompatibility, stability, low toxicity, and proficient exchange of molecular cargos make exosomes prime candidates for tissue engineering and regenerative medicine. Exploring the functions and molecular payloads of exosomes can facilitate tissue regeneration therapies and provide mechanistic insight into paracrine modulation of cellular activities. In this review, we summarize the current knowledge of exosome biogenesis, composition, and isolation methods. We also discuss emerging healing properties of exosomes and exosomal cargos, such as microRNAs, in brain injuries, cardiovascular disease, and COVID-19 amongst others. Overall, this review highlights the burgeoning roles and potential applications of exosomes in regenerative medicine.

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“…A suction rectal biopsy was taken prior to surgery to con rm the diagnosis. HSCR diagnoses were further con rmed via pathology test after surgery [13]. Table 2 shows the basic information of all the children.…”

Section: Sample Collectionmentioning

confidence: 99%

“…Furthermore, exosomes are increasingly being evaluated as diagnostic and prognostic biomarkers for a variety of diseases [11]. Studies have shown that exosome cargoes derived from several plasmas, such as long non-coding RNAs(lncRNAs) and microRNAs(miRNAs), are dysregulated in HSCR [12,13].…”

Section: Introductionmentioning

confidence: 99%

Bioinformatics Analysis of Differentially Expressed miRNAs and Their Related Genes From Plasma-Derived Exosomes in TCA

Guo

Zhang

et al. 2022

Preprint

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Background: Hirschsprung’s disease (HSCR), a congenital disorder characterized by spasticity and narrowing of the distal colon, as well as abnormalities in peristalsis and defecation, is caused by a deficit of the enteric nervous system of the distal gut. Total colonic aganglionosis (TCA) is a rare type of HSCR. This study aimed to find key microRNAs (miRNAs) and their associated genes from plasma-derived exosomes, as potential biomarkers to study TCA pathogenesis.Results: Plasma-derived exosomal miRNA sequencing of samples from 9 children with HSCR and 10 matched children without HSCR revealed that 62 miRNAs were differentially expressed in plasma-derived exosomes, of which 31 were up-regulated and 31 were down-regulated. The target genes of DEM are predicted from three common databases.There were 652 DEM target gene pairs for the 31 DEMs that were up-regulated and 234 pairs for the 31 DEMs that were down-regulated. Furthermore, 4 DEMs: miR-106b-5p, miR-205-5p, miR-375-3p and miR-34a-5p were differentially expressed between other HSCR types and TCA.Conclusions: Four key miRNAs (miR-106b-5p, miR-205-5p, miR-375-3p, and miR-34a-5p) and their interacting four key genes AF4/FMR2 family member 4 (AFF4), myotubularin-related protein 9 gene (MTMR9), POU domain class 2 transcription factor 1 (POU2F1), and WW-and-C2-domain-containing(WWC2) may be involved in the pathogenesis of TCA.

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Molecular Function Predictions and Diagnostic Value Analysis of Plasma Exosomal miRNAs in Hirschsprung’s Disease

Cited by 11 publications

References 41 publications

Plasma-Derived Exosomal hsa-miR-4488 and hsa-miR-1228-5p: Novel Biomarkers for Dermatomyositis-Associated Interstitial Lung Disease with Anti-Melanoma Differentiation-Associated Protein 5 Antibody-Positive Subset

Plasma-Derived Exosomal hsa-miR-4488 and hsa-miR-1228-5p: Novel Biomarkers for Dermatomyositis-Associated Interstitial Lung Disease with Anti-Melanoma Differentiation-Associated Protein 5 Antibody-Positive Subset

Mesenchymal Stem Cell-Derived Exosomes: Applications in Regenerative Medicine

Bioinformatics Analysis of Differentially Expressed miRNAs and Their Related Genes From Plasma-Derived Exosomes in TCA

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