Molecular fingerprinting of Staphylococcus aureus isolated from patients with osteomyelitis in Argentina and clonal distribution of the cap5(8) genes and of other selected virulence genes

Lattar, Santiago M.; Tuchscherr, Lorena; Centrón, Daniela; Becker, Karsten; Predari, Silvia C.; Buzzola, Fernanda R.; Robinson, D. Ashley; Sordelli, Daniel O.

doi:10.1007/s10096-012-1596-8

Cited by 17 publications

(17 citation statements)

References 42 publications

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“…This clone was shown to have a better adaptation to animals than to humans and has been associated with bovine mastitis [19] with little impact on humans [20]. In the present study, the two CC97 S. aureus strains were isolated in DFOM as previously observed [21], suggesting a bone tropism of these clones of animal origin. The adaptation of animal S. aureus isolates to humans is a worrisome problem and should be carefully monitored.…”

supporting

confidence: 49%

First report of the predominance of clonal complex 398 Staphylococcus aureus strains in osteomyelitis complicating diabetic foot ulcers: a national French study

Senneville

Brière

Neut

et al. 2014

Clinical Microbiology and Infection

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Staphylococcus aureus is the most common pathogen cultured from diabetic foot infection including diabetic foot osteomyelitis. This French multicentre study determined the genetic content of S. aureus isolated from 157 consecutive cases admitted to 12 diabetic foot centres between 2008 and 2011. We describe for the first time the emergence of the CC398 methicillin-susceptible S. aureus clone, the main clone in diabetic foot osteomyelitis, and its tropism for bone. This clone spreads to humans from an animal source through its intrinsic virulence. This adaptation of S. aureus isolates looks to be a worrisome problem and should be carefully monitored.

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supporting

confidence: 49%

First report of the predominance of clonal complex 398 Staphylococcus aureus strains in osteomyelitis complicating diabetic foot ulcers: a national French study

Senneville

Brière

Neut

et al. 2014

Clinical Microbiology and Infection

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“…In addition, 8 clinical isolates related to USA300 and USA100 clones (Table 1), which were identified previously by spa typing, MLST and SCCmec typing (Lattar et al, 2012) were included in the study. S. aureus SA113 and Reynolds CP5 null (Δ cap5 ) (Watts et al, 2005) strains were also included as reference controls.…”

Section: Methodsmentioning

confidence: 99%

“…Different SCCmec types were established by specific PCR assays. The spa types from sequences of the protein A (spa) repeat region were assessed from the Ridom spa server (http://spa.ridom.de) (Lattar et al, 2012) .…”

Section: Methodsmentioning

confidence: 99%

“…While asymptomatic colonization of S. aureus does not necessarily lead to illness, the loss of the mucosal or epithelial surface integrity can be responsible for S. aureus diverse diseases (Fitzpatrick et al, 2005). Persistent and difficult-to-eradicate infections can be caused by both methicillin-susceptible S. aureus (MSSA) (Lattar et al, 2012) and methicillin-resistant S. aureus (MRSA).…”

Section: Introductionmentioning

confidence: 99%

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The Active Component of Aspirin, Salicylic Acid, Promotes Staphylococcus aureus Biofilm Formation in a PIA-dependent Manner

et al. 2017

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Aspirin has provided clear benefits to human health. But salicylic acid (SAL) -the main aspirin biometabolite- exerts several effects on eukaryote and prokaryote cells. SAL can affect, for instance, the expression of Staphylococcus aureus virulence factors. SAL can also form complexes with iron cations and it has been shown that different iron chelating molecules diminished the formation of S. aureus biofilm. The aim of this study was to elucidate whether the iron content limitation caused by SAL can modify the S. aureus metabolism and/or metabolic regulators thus changing the expression of the main polysaccharides involved in biofilm formation. The exposure of biofilm to 2 mM SAL induced a 27% reduction in the intracellular free Fe2+ concentration compared with the controls. In addition, SAL depleted 23% of the available free Fe2+ cation in culture media. These moderate iron-limited conditions promoted an intensification of biofilms formed by strain Newman and by S. aureus clinical isolates related to the USA300 and USA100 clones. The slight decrease in iron bioavailability generated by SAL was enough to induce the increase of PIA expression in biofilms formed by methicillin-resistant as well as methicillin-sensitive S. aureus strains. S. aureus did not produce capsular polysaccharide (CP) when it was forming biofilms under any of the experimental conditions tested. Furthermore, SAL diminished aconitase activity and stimulated the lactic fermentation pathway in bacteria forming biofilms. The polysaccharide composition of S. aureus biofilms was examined and FTIR spectroscopic analysis revealed a clear impact of SAL in a codY-dependent manner. Moreover, SAL negatively affected codY transcription in mature biofilms thus relieving the CodY repression of the ica operon. Treatment of mice with SAL induced a significant increase of S aureus colonization. It is suggested that the elevated PIA expression induced by SAL might be responsible for the high nasal colonization observed in mice. SAL-induced biofilms may contribute to S. aureus infection persistence in vegetarian individuals as well as in patients that frequently consume aspirin.

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“…It has been associated with an increased risk of infective endocarditis, 10 increased hematogenous complications 3 and persistent S. aureus bacteremia. 15 In Argentina, methicillin-sensitive S. aureus (MSSA) strains belonging to the ST30 lineage have also been recovered from cases of osteomyelitis in a study conducted by Lattar et al 6 Among the published data of necrotizing pneumonia due to CA-MRSA, different clones have been described, e.g. ST59-SCCmecIV-spat437-PVL positive, ST910-SCCmecIV-spat318, ST398-SCCmecV-spat034, ST1409-SCCmecIV-spat653, 1 ST80, 11 showing that there would not be a unique MRSA clone associated with this disease, and could correspond to the most prevalent CA-MRSA clone in each region.…”

Section: Neumonía Necrotizante Adquirida En La Comunidad Causada Por mentioning

confidence: 98%

Community-acquired necrotizing pneumonia caused by methicillin-resistant Staphylococcus aureus ST30-SCCmecIVc-spat019-PVL positive in San Antonio de Areco, Argentina

Fernandez

Murzicato²,

Sandoval³

et al. 2015

Revista Argentina de Microbiología

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Community-acquired methicillin-resistant Staphylococcus aureus is the first cause of skin and soft tissue infections, but can also produce severe diseases such as bacteremia, osteomyelitis and necrotizing pneumonia. Some S. aureus lineages have been described in cases of necrotizing pneumonia worldwide, usually in young, previously healthy patients. In this work, we describe a fatal case of necrotizing pneumonia due to community-acquired methicillin-resistant S. aureus clone ST30-SCCmecIVc-spat019-PVL positive in an immunocompetent adult patient.

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Molecular fingerprinting of Staphylococcus aureus isolated from patients with osteomyelitis in Argentina and clonal distribution of the cap5(8) genes and of other selected virulence genes

Cited by 17 publications

References 42 publications

First report of the predominance of clonal complex 398 Staphylococcus aureus strains in osteomyelitis complicating diabetic foot ulcers: a national French study

First report of the predominance of clonal complex 398 Staphylococcus aureus strains in osteomyelitis complicating diabetic foot ulcers: a national French study

The Active Component of Aspirin, Salicylic Acid, Promotes Staphylococcus aureus Biofilm Formation in a PIA-dependent Manner

Community-acquired necrotizing pneumonia caused by methicillin-resistant Staphylococcus aureus ST30-SCCmecIVc-spat019-PVL positive in San Antonio de Areco, Argentina

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