Molecular fingerprinting of Mycobacterium tuberculosis strains isolated in Vietnam using IS 6110 as probe

Tuyen, Le Thi Kim; Hoa, Bach Khanh; Ly, Ho Minh; Van, Le Ngoc; Lan, Nguyễn Thị Ngọc; Chevrier, D.; Guesdon, Jean‐Luc

doi:10.1054/tuld.2000.0234

Cited by 12 publications

(9 citation statements)

References 32 publications

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“…Generally, the Beijing family strains do not appear to be inherently MDR (1,21,30). In the present study, 10 of 39 (25.6%) pansusceptible strains, 4 of 10 (40%) STR-monoresistant strains, and 1 of 4 INH-monoresistant strains belonged to the Beijing type.…”

Section: Discussionmentioning

confidence: 78%

“…Twenty-five additional DR spacers have recently been described (33), but use of these sequences has provided only slight improvement in the ability to discriminate among strains of M. tuberculosis (24,33). Spoligotyping is less discriminatory than IS6110-based fingerprinting, especially for strains with high IS6110 copy numbers (1,8,26,30), and is most suitable for the preliminary discrimination of strains. Subsequently, the strains with identical spoligoprofiles may be subtyped by IS6110 RFLP analysis, which is widely used as a standardized epidemiological typing technique (30,32).…”

Section: Discussionmentioning

confidence: 99%

“…Spoligotyping is less discriminatory than IS6110-based fingerprinting, especially for strains with high IS6110 copy numbers (1,8,26,30), and is most suitable for the preliminary discrimination of strains. Subsequently, the strains with identical spoligoprofiles may be subtyped by IS6110 RFLP analysis, which is widely used as a standardized epidemiological typing technique (30,32).…”

Section: Discussionmentioning

confidence: 99%

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High Prevalence of KatG Ser315Thr Substitution among Isoniazid-Resistant Mycobacterium tuberculosis Clinical Isolates from Northwestern Russia, 1996 to 2001

Mokrousov

Narvskaya

Otten

et al. 2002

Antimicrob Agents Chemother

163

109

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A total of 204 isoniazid (INH)-resistant strains of Mycobacterium tuberculosis isolated from different patients in the northwestern region of Russia from 1996 to 2001 were screened by a PCR-restriction fragment length polymorphism (RFLP) assay. This assay uses HapII cleavage of an amplified fragment of the katG gene to detect the transversion 315AGC→ACC (Ser→Thr), which is associated with INH resistance. This analysis revealed a 93.6% prevalence of the katG S315T mutation in strains from patients with both newly and previously diagnosed cases of tuberculosis (TB). This mutation was not found in any of 57 INH-susceptible isolates included in the study. The specificity of the assay was 100%; all isolates that contained the S315T mutation were classified as resistant by a culture-based susceptibility testing method. The Beijing genotype, defined by IS6110-RFLP analysis and the spacer oligonucleotide typing (spoligotyping) method, was found in 60.3% of the INH-resistant strains studied. The katG S315T shift was more prevalent among Beijing genotype strains than among non-Beijing genotype strains: 97.8 versus 84.6%, respectively, for all isolates, including those from patients with new and previously diagnosed cases, isolated from 1999 to 2001 and 100.0 versus 86.5%, respectively, for isolates from patients with new cases isolated from 1996 to 2001. The design of this PCR-RFLP assay allows the rapid and unambiguous identification of the katG 315ACC mutant allele. The simplicity of the assay permits its implementation into routine practice in clinical microbiology laboratories in regions with a high incidence of TB where this mutation is predominant, including northwestern Russia

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Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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High Prevalence of KatG Ser315Thr Substitution among Isoniazid-Resistant Mycobacterium tuberculosis Clinical Isolates from Northwestern Russia, 1996 to 2001

Mokrousov

Narvskaya

Otten

et al. 2002

Antimicrob Agents Chemother

163

109

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“…IS 6110 copy numbers in other clusters were those between these two families. An earlier study demonstrated that copy numbers of northern strains in Vietnam were relatively smaller than those of southern strains in which typical Beijing genotypes are frequently observed [6]. We will need to further characterize the strains originating from the Hanoi area in future genotypic studies.…”

Section: Discussionmentioning

confidence: 99%

“…It remains unclear whether entire profiles of MTB isolates obtained in one area are equally useful throughout the country. An earlier report [6] suggested differences in genotypes and drug susceptibility patterns between isolates obtained in distant regions of Vietnam.…”

Section: Introductionmentioning

confidence: 99%

Clonal expansion of Mycobacterium tuberculosis isolates and coexisting drug resistance in patients newly diagnosed with pulmonary tuberculosis in Hanoi, Vietnam

et al. 2013

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BackgroundNewly diagnosed patients without anti-tuberculosis (TB) treatment histories have not often undergone drug susceptibility testing (DST), but have received the standard treatment regimen without information about their DST profiles in many countries with inadequate resources.MethodsWe collected 346 clinical isolates from previously untreated patients with smear-positive active TB in Hanoi, the capital of Vietnam. Of these, 339 were tested for susceptibility to four first-line anti-TB drugs, including isoniazid (INH), rifampicin (RMP), streptomycin (SM), and ethambutol (EMB), using the proportion method. A pyrazinamidase (PZase) test was used to assess pyrazinamide (PZA) resistance. Results of the culture-based drug susceptibility tests were confirmed by those from reverse hybridization-based line probe assays (LiPAs) that detected mutations associated with RMP, INH, PZA, and fluoroquinolone (FQ) resistance. To investigate a diversity of these strains, IS6110-probed restriction fragment length polymorphisms (RFLPs) were analyzed. Nucleotide sequences for furA-katG and fabG1-inhA operons, transcription units responsible for INH resistance, were also determined.ResultsOf the isolates tested, 127 (37.5%) were resistant to at least one of the four drugs, which included 93 (27.4%) isolates that were resistant to INH. RFLP analysis identified four clusters defined by similarity of the band patterns, which accounted for 46.1% of the tested isolates. Among the clustered isolates, 37.7% were resistant to INH, most of which (85.4%) carried a g944c mutation, which causes an S315T amino acid substitution, in the katG gene.ConclusionsOur results suggest that drug-resistant strains, particularly those with INH resistance characterized by a single mutation, S315T, are spreading in Hanoi, Vietnam. When RMP resistance is combined with this setting, patients are not easily cured by conventional short-term treatment. We will need to carefully monitor these trends and search for the origins and transmission routes of these strains.

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Mycobacterium tuberculosis Beijing genotype: A template for success

et al. 2011

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Molecular fingerprinting of Mycobacterium tuberculosis strains isolated in Vietnam using IS 6110 as probe

Cited by 12 publications

References 32 publications

High Prevalence of KatG Ser315Thr Substitution among Isoniazid-Resistant Mycobacterium tuberculosis Clinical Isolates from Northwestern Russia, 1996 to 2001

High Prevalence of KatG Ser315Thr Substitution among Isoniazid-Resistant Mycobacterium tuberculosis Clinical Isolates from Northwestern Russia, 1996 to 2001

Clonal expansion of Mycobacterium tuberculosis isolates and coexisting drug resistance in patients newly diagnosed with pulmonary tuberculosis in Hanoi, Vietnam

Mycobacterium tuberculosis Beijing genotype: A template for success

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