Molecular fingerprinting of hybrids and assessment of genetic purity of hybrid seeds in rice using microsatellite markers

Nandakumar, N.; Singh, Ashok Kumar; Sharma, Richa; Mohapatra, Trilochan; Prabhu, K. V.; Zaman, F. U.

doi:10.1023/b:euph.0000032706.92360.c6

Cited by 93 publications

(74 citation statements)

References 22 publications

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“…As we know, molecular fingerprinting is an effective and accurate way to identify crop varieties (Nandakumar et al, 2004). In this study, we found that the SSR marker Bmac0303 could distinguish the 3 new barley cultivars from other barley landraces, and we could then protect the breeder rights of the 3 barley cultivars by this locus.…”

Section: Ssr Markers In New Variety Protectionmentioning

confidence: 76%

Genetic diversity analysis of barley landraces and cultivars in the Shanghai region of China

Chen

Lu²,

Zou³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. We analyzed the genetic diversity of 115 barley germplasms, including 112 landraces and three new barley cultivars grown in the Shanghai region, using a set of 11 SSR markers. Sixtysix alleles were observed at the 11 SSR loci, ranged from three to ten, with a mean of six alleles per locus. The polymorphism information content ranged from 0.568 to 0.853, with a mean of 0.732, indicating considerable genetic variation in barley in the Shanghai area. Clustering analysis indicated that these barley accessions could be divided into two categories (A and B). Ninety-seven six-rowed barley cultivars were classified in the A category; sixteen two-rowed and two six-rowed barley cultivars were classified in the B category. This demonstrated genetic differences between two-rowed and six-rowed barley varieties. In addition, we found that the three new barley cultivars are closely related.

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Section: Ssr Markers In New Variety Protectionmentioning

confidence: 76%

Genetic diversity analysis of barley landraces and cultivars in the Shanghai region of China

Chen

Lu²,

Zou³

et al. 2012

Genet. Mol. Res.

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“…As a general knowledge and fact, molecular fingerprinting is an effective and accurate way to identify crop varieties (Nandakumar et al, 2004). In this study, it was found that the SSR marker have at least distinguished the released barley cultivars from other elite barley cultivars.…”

Section: Ssr Markers In New Variety Protectionmentioning

confidence: 73%

Assessment of genetic diversity among released and elite Ethiopian barley genotypes using simple sequence repeat (SSR) markers

Misganaw¹,

Kidane²,

Tesfu³

2017

Afr. J. Plant Sci.

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Barley is a major cereal grown widely and used in several food products, beverage production and animal feed. Being the fourth most important cereal crop in the world and the fifth rank in Ethiopia, it is a cash crop and used as a source of malt by the brewery industries, as food for human and feed for animals. Genetic diversity assessment is a key component in breeding programs. High level of polymorphism, codominant and multi allelic nature of simple sequence repeats (SSRs) markers make them preferable for diversity analysis in plant species. In this study, 22 SSRs markers were used to characterize the genetic diversity of 39 released and elite barley varieties collected from barley breeding program in Ethiopia. The amplification of SSRs loci were obtained for 35 primer pairs and only 22 of them showed clear polymorphic patterns which produced a total of 73 alleles with an average of 5 alleles per locus. The data generated by these informative primers were sufficient to discriminate the analysed barley genotypes. Based on the dissimilarity matrices ranging from 0.11 to 0.58, the genotypes were grouped into three major groups. The calculated polymorphism information content (PIC) values ranges from 0.17 to 0.60 with an average of 0.47 which shows the importance of the markers for future diversity analysis of barley. Locus HVACL1 and HVM36 shows higher PIC and locus HVBDHN7 shows lower PIC in this characterized barley genotype. This result will be useful for barley germplasm management and improvement in terms of biodiversity protection and design of new crosses for future breeding purpose.

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“…Identification of male and female parent specific markers will allow differentiation of true hybrids from selfed parental and outcrossed lines in F 1 because it contains DNA from both the parents. Molecular markers, such as RAPD, ISSR, SSR, AFLP and RFLP have been used in cultivar fingerprinting, seed purity testing and germplasm identification for many crops (Crockett et al 2000, Dongre and Parkhi 2005, Liu et al 2004, Nandakumar et al 2004. Among the various molecular markers currently available, simple sequence repeats (SSR), also known as microsatellites, are widely accepted as reliable.…”

Section: Introductionmentioning

confidence: 99%

Identification of true hybrid progenies in cassava using simple sequence repeat (SSR) markers

2013

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For the maping of Cassava mosaic disease (CMD) resistance gene, CO2 and MNga-1 parents were used for hybridization to develop F 1 hybrid seeds and 153 progenies were planted in the field for genotyping. Out of the 75 SSR primers used for parental polymorphism, 57 produced polymorphic bands between parents and these were used to screen the 153 progenies of the cross for genotyping. Out of these 57 SSR primer pairs SSRY32, SSRY36, SSRY83, SSRY339, NS149 and NS890 loci produced single marker which clearly distinguished the parents. Based on the said six primers, 12 progenies out of 153 were identified as self pollinated seeds of CO2 female parent. So these types of SSR markers were very much useful for identification of true hybrid seedlings in the early stage of growth and genotyping of the progenies more accurately for gene mapping.

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Molecular fingerprinting of hybrids and assessment of genetic purity of hybrid seeds in rice using microsatellite markers

Cited by 93 publications

References 22 publications

Genetic diversity analysis of barley landraces and cultivars in the Shanghai region of China

Genetic diversity analysis of barley landraces and cultivars in the Shanghai region of China

Assessment of genetic diversity among released and elite Ethiopian barley genotypes using simple sequence repeat (SSR) markers

Identification of true hybrid progenies in cassava using simple sequence repeat (SSR) markers

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