“…If the presence of high number of apyrene sperm can delay female remating, as suggested in the green-vined white butterfly, Pieris napi (L.) (Cook & Wedell, 1999), and the pipevine swallowtail butterfly, Byasa alcinous (Klug) (Konagaya et al, 2016), increase of apyrene sperm number produced and ejaculated would be advantageous for males, because males can maximize fertilization success. However, recent studies using molecular biology suggest that apyrene sperm do not affect sperm completion directly (Mongue et al, 2018) and function for sperm migration in female reproductive organs (Sakai et al, 2019). In relation to sperm competition, the mechanism of how males control production, movement, and ejaculation of two types of sperm is unclear.…”