Molecular evolution of enolase.

Piast, Michał; Kustrzeba−Wójcicka, Irena; Matusiewicz, Małgorzata; Banaś, Teresa

doi:10.18388/abp.2005_3466

Cited by 84 publications

(45 citation statements)

References 22 publications

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“…In general, the results were in good agreement with traditional taxonomy, with Ssc-eno and other arthropoda species clustered into a group within the phylum Arthropoda, then with other eukaryotic species, and finally prokaryotic species, such as protozoa and bacteria. In particular, enolases from vertebrates were characterized by greater intraspecific, rather than interspecies, similarities, demonstrated as separate monophyletic branches for three different enolase isoforms (α, β, and γ) reflecting their different functions; this results was consistent with previous study (Piast et al, 2005 ).…”

Section: Resultssupporting

confidence: 92%

Serodiagnostic Potential of Alpha-Enolase From Sarcoptes scabiei and Its Possible Role in Host-Mite Interactions

Huang²,

Dong

et al. 2018

Front. Microbiol.

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Infestation of the epidermis with the highly contagious ectoparasite, Sarcoptes scabiei, causes scabies, which is characterized by intense itching, pruritus, and secondary infection. This condition affects humans, livestock, and wildlife worldwide, incurring large economic losses and reducing the quality of human life. In the present study, we cloned the alpha-enolase, a key enzyme in the glycolytic and gluconeogenesis pathways, from S. scabiei var. cuniculi, characterized it and produced soluble recombinant enolase protein (rSsc-eno). We determined the localization of Ssc-eno in isolated mites and mites in lesioned skin. The results showed that native enolase was intensely localized in the tegument of the mouthparts, the entire legs, and the whole mites' body, as well as in the gut and reproduction system. Interestingly, we found that native enolase was widely distributed in mites in lesioned skin, with obvious high protein intensity compared with isolated mites. Building on good immunoreactivity, an indirect enzyme-linked immunosorbent assay (ELISA) based on rSsc-eno showed 92% sensitivity and 95.8% specificity, compared with other indirect ELISA in this study, rSsc-eno based ELISA is better in detecting scabies in rabbits. Besides, this method can detect S. scabiei infection as early as 1 week post infection. Compared with other detection methods, such as traditional microscopic examination and recently published universal conventional PCR, rSsc-eno ELISA was more effective to detect early infection in rabbits. Additionally, in vitro incubation experiments demonstrated the concentration-dependent acaricidal activity of rabbit anti-rSsc-eno sera against larval mites, suggested its potential as a vaccine candidate.

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Section: Resultssupporting

confidence: 92%

Serodiagnostic Potential of Alpha-Enolase From Sarcoptes scabiei and Its Possible Role in Host-Mite Interactions

Huang²,

Dong

et al. 2018

Front. Microbiol.

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“…We also identified five underexpressed proteins in IBS (ENO1, transgelin, serpin peptidase inhibitor B5, alpha 1 cardiac muscle actin, and 40S ribosomal protein SA). ENO1 is a multifunctional enzyme that not only catalyzes the conversion of 2‐phosphoglycerate to phosphoenolpyruvate in the presence of Mg 2+ ions in glycolysis 60, but also plays a role in other processes such as growth control, hypoxic tolerance, and allergic responses, as well as bacterial and fungal infections, cancer, neurologic conditions, and autoimmunity 61.…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of colonic mucosa in a rat model of irritable bowel syndrome

Ding¹,

Lü²,

Chen³

et al. 2010

Proteomics

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Irritable bowel syndrome (IBS) is one of the most common functional disorders of the gastrointestinal tract. It is characterized by abdominal pain and changes in bowel habits. Various studies have investigated the pathophysiologic processes underlying IBS, but the mechanism remains poorly understood. In the present study, we established an IBS model and identified differentially expressed proteins in colon tissue of IBS rats compared with healthy controls by 2-D gel electrophoresis, MALDI-TOF-MS, and Western blot analysis. Our results showed that 13 of the 1396 protein spots on 2-D gel were differently expressed between the IBS and control groups. Ontological analysis of these proteins revealed primary roles in catalytic activity (protein disulfide-isomerase A3, glyoxalase I, cathepsin S, alpha-enolase), structural support (cytokeratin 8), antioxidant activity (peroxiredoxin-6), protein binding (transgelin, serpin peptidase inhibitor B5), and signal transduction (40S ribosomal protein SA). Protein disulfide-isomerase A3 and cytokeratin 8 overexpression in IBS were confirmed by Western blot. The findings indicate that multiple proteins are involved in IBS processes that influence intestinal tract immunity, inflammation, and nerve regulation. Our study provides useful candidate genes and proteins for further investigation.

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“…In this study, glycolytic enzyme enolase 1 (a-enolase, a homodimer of 47-48 kDa) was identified as an up-regulated protein. The enolase 1 enzyme catalyzes the conversion of 2-phosphoglycerate to phosphoenolopyruvate in the presence of Mg 21 ions [29], and plays a role in various processes such as growth control, hypoxic tolerance, and allergic responses, as well as several types of diseases, such as bacterial, fungal, cancer, neurologic, and autoimmunity [30]. It exists as a multienzyme complex with other glycolytic enzymes, such as pyruvate kinase, phosphoglycerate mutase, muscle creatine kinase, and aldolase, in the cytoplasm [31,32].…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of human proximal tubular cells exposed to high glucose concentrations

Kim

2008

Proteomics Clinical Apps

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Hyperglycemia is a major key factor in the pathogenesis of microvascular complications of diabetes, including diabetic nephropathy (DN). Most studies to date have focused on the glomerular abnormalities found in DN. However, nephromegaly in the early stages of diabetes and the correlation of tubulointerstitial pathology rather than glomerular pathology with declining renal function in DN suggests the involvement of the tubulointerstitium. The etiology of the tubulointerstitial pathology in DN, however, is not fully understood. In this study, to understand the DN pathways, we constructed an initial 2-DE reference map for primitively cultured human proximal tubule (HK-2) cell in the presence of 5 mM and 25 mM glucose, which correspond to blood glucose concentrations during the normal and hyperglycemia conditions, respectively. Differentially expressed HK-2 cell cellular proteins at the high glucose concentration were identified via ESI-Q-TOF MS/MS and confirmed by Western blotting; enolase 1 (up-regulated) and lactate dehydrogenase (down-regulated). The regulation of these proteins will help in understanding DN mechanism through the glycolysis metabolic pathways in high glucose stimulated HK-2 cells.

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Molecular evolution of enolase.

Cited by 84 publications

References 22 publications

Serodiagnostic Potential of Alpha-Enolase From Sarcoptes scabiei and Its Possible Role in Host-Mite Interactions

Serodiagnostic Potential of Alpha-Enolase From Sarcoptes scabiei and Its Possible Role in Host-Mite Interactions

Proteomic analysis of colonic mucosa in a rat model of irritable bowel syndrome

Proteomic analysis of human proximal tubular cells exposed to high glucose concentrations

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