Molecular Evolution Inferred from Immunological Cross-reactivity of Immunoglobulin G among Chiroptera and Closely Related Species

Omatsu, Tsutomu; Ishii, Yoshiyuki; Kyuwa, Shigeru; Milanda, Elizabeth G.; Terao, Keiji; Yoshikawa, Yasuhiro

doi:10.1538/expanim.52.425

Cited by 11 publications

(12 citation statements)

References 16 publications

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“…Anti-bat ( Rousettus aegyptiacus ) rabbit IgG strongly cross-reacts with IgGs of other bat species, including insectivorous bats ( 8 ). Bat serum samples were 4-fold serially diluted (1:100–1:6,400) and tested by using IgG ELISAs.…”

mentioning

confidence: 99%

Reston Ebolavirus Antibodies in Bats, the Philippines

Taniguchi

Watanabe

Masangkay

et al. 2011

Emerg. Infect. Dis.

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mentioning

confidence: 99%

Reston Ebolavirus Antibodies in Bats, the Philippines

Taniguchi

Watanabe

Masangkay

et al. 2011

Emerg. Infect. Dis.

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“…Although less commonly used, the comparison of serum protein epitopes is a reliable alternative indicator of evolutionary relatedness among species (6,14,16,30). Ig antibodies are often the target protein of choice for these cross-reactivity based relatedness studies, because Ig antibodies are large protein molecules carrying multiple antigenic epitopes.…”

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confidence: 99%

“…Moreover, Ig antibodies are found in sera from all vertebrate species (10). They form an essential component of the humoral immune system and are therefore sufficiently conserved to allow for comparison across a broad range of species (5,8,30). However, high-quality blood and serum samples are needed to allow for the evaluation of Ig cross-reactivity.…”

mentioning

confidence: 99%

“…In contrast, a MAb binds one specific epitope on the target protein. The degree of cross-reactivity of either type of antibody with the target protein among species reflects the degree of conservation and therefore genetic proximity of the various species (8,30). However, since the polyAb mixture binds multiple epitopes, a broader spectrum of cross-reactivity would be expected with polyAb than with a MAb.…”

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confidence: 99%

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Cross-Reactivity between Immunoglobulin G Antibodies of Whales and Dolphins Correlates with Evolutionary Distance

Nollens

Ruíz

Walsh

et al. 2008

Clin Vaccine Immunol

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Growing morphological and molecular evidence indicates that the porpoises, dolphins, and whales evolved within the even-toed ungulates, formerly known as Artiodactyla. These animals are now grouped in the Cetartiodactyla. We evaluated the antigenic similarity of the immunoglobulin G (IgG) molecules of 15 cetacean species and the domestic cow. The similarity was scored using three distinct antibodies raised against bottlenose dolphin (Tursiops truncatus) IgG in a Western blot, an indirect enzyme-linked immunosorbent assay (ELISA), and a competitive ELISA format. A score was generated for the genetic distance between each species and T. truncatus using the cytochrome b sequence. Each antibody displayed a distinct pattern of reactivity with the IgG antibodies of the various species. The monoclonal antibody (MAb) specific for the ␥ heavy chain of T. truncatus was reactive with all monodontids, delphinids, and phocoenids. The light-chain-specific MAb reacted with IgG of delphinoid and phocoenid species and one of the two mysticete species tested. The polyclonal antibody was broadly cross-reactive across all cetaceans and the domestic cow. Using the MAb specific for the ␥ heavy chain, the degree of IgG cross-reactivity ranged from less than 17% for the mysticetes to 106% for killer whale Orcinus orca. The IgG in beaked whale and baleen whale sera was significantly less cross-reactive with bottlenose dolphin IgG than sera from other toothed whales. A strong negative correlation was demonstrated between antigenic cross-reactivity of IgG molecules and the genetic distance of their hosts. The data generated will be useful for the development of clinical serodiagnostics in diverse cetacean species.

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“…In our study, the ELISA system using biotinylated anti-bat IgG rabbit sera was developed. We used polyclonal anti-bat IgG rabbit sera reported in our previous paper [69]. The antibody reacted only with bat IgG, not with IgG of other mammalian species.…”

Section: Epidemiological Study Of Batsmentioning

confidence: 99%

Biological characters of bats in relation to natural reservoir of emerging viruses

Omatsu

Watanabe

Akashi

et al. 2007

Comparative Immunology, Microbiology and Infectious Diseases

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Many investigators focused on bats (Chiroptera) for their specific character, i.e. echolocation system, phylogenic tree, food practice and unique reproduction. However, most of basic information about the vital functions related to anti-viral activity has been unclear. For evaluating some animals as a natural reservoir or host of infectious pathogens, it is necessary that not only their immune system but also their biology, the environment of their living, food habits and physiological features should be clarified and they should be analyzed from these multi-view points. The majority of current studies on infectious diseases have been conducted for the elucidation of viral virulence using experimental animals or viral gene function in vitro, but in a few case, researchers focused on wild animal itself. In this paper, we described basic information about bats as follows; genetic background, character of the immunological factors, histological character of immune organs, the physiological function and sensitivity of bat cells to viral infection.

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Molecular Evolution Inferred from Immunological Cross-reactivity of Immunoglobulin G among Chiroptera and Closely Related Species

Cited by 11 publications

References 16 publications

Reston Ebolavirus Antibodies in Bats, the Philippines

Reston Ebolavirus Antibodies in Bats, the Philippines

Cross-Reactivity between Immunoglobulin G Antibodies of Whales and Dolphins Correlates with Evolutionary Distance

Biological characters of bats in relation to natural reservoir of emerging viruses

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