Molecular evolution and epidemiology of dengue-3 viruses

Abstract

The nucleic acid sequences of the pre-membrane/ membrane and envelope protein genes of 23 geographically and temporally distinct dengue (DEN)-3 viruses were determined. This was accomplished by reverse transcriptase-PCR amplification of the structural genes followed by automated DNA sequence analysis. Comparison of nucleic acid sequences revealed that similarity among the viruses was greater than 90 %. The similarity among deduced amino acids was between 95 % and 200 %, and in many cases identical amino acid substitutions occurred among viruses from similar geographical regions. Alignment of nucleic acid sequences followed by parsimony analysis allowed the generation of phylogenetic trees, demonstrating that geographically independent evolution of DEN-3 viruses had occurred. The DEN-3 viruses were separated into four genetically distinct subtypes. Subtype I consists of viruses from Indonesia, Malaysia, the Philippines and the South Pacific islands; subtype II consists of viruses from Thailand; subtype III consists of viruses from Sri Lanka, India, Africa and Samoa; subtype IV consists of viruses from Puerto Rico and the 1965 Tahiti virus. Phylogenetic analysis has also contributed to our understanding of the molecular epidemiology and worldwide distribution of DEN-3 viruses.

Correlation of disease spectrum among four Dengue serotypes: a five years hospital based study from India

“…Changes in the envelope protein in particular are suggested to alter host cell susceptibility, thereby affecting virus replication and disease severity. 28 The nucleotide sequence comparison of E-NS1 region of fi ve Den-3 strains from present study with published sequences using BLAST revealed that irrespective of the year in which samples were collected, all of them belonged to genotype III. The strains isolated in our study showed ~98% homology with subtype-III of Den-3 strains isolated during epidemics in 1984 from India, in 1985 from Mozambique, in 1989, 1991 and 2000 from Sri Lanka, and in 2000 from Martinique.…”

“…The strains isolated in our study showed ~98% homology with subtype-III of Den-3 strains isolated during epidemics in 1984 from India, in 1985 from Mozambique, in 1989, 1991 and 2000 from Sri Lanka, and in 2000 from Martinique. 28,29 The identifi cation of Den-3 during the outbreak in India during 2003 and its continued presence in later years indicates its resurgence in a dominant form and, thus, a matter of great concern. Constant and thorough epidemiological surveillance is mandatory to control the invasion and spread of dengue viruses.…”

Dengue virus type 3 in Rio de Janeiro, Brazil

“…The above-mentioned data demonstrate the importance of on-going monitoring of samples of DEN viruses circulating in the country in order to (Lanciotti et al 1994, Anonymus 1995.…”

“…Dengue (DEN) viruses belong to the family Flaviviridae, genus Flavivirus, and occur as four antigenically related but distinct serotypes designated DEN-1, 2, 3 and 4 (Westaway et al 1985). Although all dengue serotypes are responsible for both DF or DHF/DSS, dengue subtyping methods have been used for epidemiologic and pathogenesis investigations, since genetic differences could reveal important information related to the disease (Lanciotti et al 1994, Rico-Hesse et al 1997.…”

Infection of the central nervous system with dengue virus 3 genotype I causing neurological manifestations in Brazil

“…Phylogenetic studies have classifi ed DENV-3 into fi ve genotypes (I, II, III, IV, and V) based on their genetic diversity (5) . Our laboratory has isolated and characterized DENV-3 genotype I from patients and mosquitoes in different cities of Minas Gerais (Figure 1), most recently (2013) in Contagem, MG (unpublished data) (6) (7) .…”