Molecular evidence supports the separation of Lentinula edodes from Lentinus and related genera

Abstract

Molecular evidence supports the separation of Lentinula edodes from Lentinus and related genera. Can. J. Bot. 70: 2446-2452.Restriction polymorphisms in two regions of the ribosomal DNA (rDNA) repeat unit were examined in 18 strains of Lentinus, Neolentinus, Pleurotus, and the shiitake mushroom Lentinula edodes. The polymerase chain reaction was used to separately amplify the 18s rDNA and the region spanning the two internal transcribed spacers and the 5.8s ribosomal RNA gene. Amplified products were digested with a battery of 10 restriction endonucleases and the two data sets were subjected to cluster analysis. All strains of Lentinula edodes consistently exhibited identical restriction profiles that were distinct from those of the genera Lentinus, Neolentinus, and Pleurotus. The internal transcribed spacer region exhibited more variability than the 18s rDNA, giving distinctive profiles for two strains of Lentinus tigrinus and for one strain of Neolerltin~is lepide~cs. Similarity coefficients were clustered with the unweighted pair group method with arithmetic average, single-linkage method, and complete-linkage method. Results from cluster analysis of the two data sets were highly congruent and tree topologies were consistent irrespective of the clustering method used. The distinctiveness of the groups was further confirmed by computing for consensus trees and cophenetic correlation coefficients. Ribosomal DNA restriction polymorphisms support the placement of the strains examined in separate taxa.

Genetic analysis of nuclear ribosomal DNA of Lentinula edodes

“…The size of the amplified ITS region was about 750 base pairs (bp). This value was similar to that reported by Molina et al (1992), who amplified the ITS region of L. edodes using the same primers and estimated the amplified ITS region to include 700 bp. The two parental monokaryons of L. edodes used in this study differed in the HinfI digestion patterns of their ITS region (Fig.…”

Phylogeny of the Genus Lentinula Based on Ribosomal DNA Restriction Fragment Length Polymorphism Analysis

“…Pegler has been revised numerous times since its original description in 1877 as Agaricus edodes Berk. Recent molecular and morphological analyses support the separation of L. edodes from the genus Lentinus (Hibbett and Vilgalys, 1993;Molina et al, 1992). Singer, given in 1941 by Singer, is still in use by some workers.…”

A new species of Lentinula (Agaricales) from Central America

“…) Pegler, several distinguishable morphological variants occur, which while sexually intercompatible (Mori et al, 1974;Shimomura et al, 1992) produce identifiable RFLP signatures (Nicholson et al, 1997) and definite phylogenetic ITS-based lineages (Hibbett et al, 1998). These Old World taxa have received much attention because of their economic importance (Chiu et al, 1999;Hibbett and Donoghue, 1996;Royse and May, 1987) along with morphological and molecular revisions to assess their taxonomic circumscription (Molina et al, 1992;Pegler, 1975Pegler, , 1983.…”