Molecular evidence of zoonotic <i>Babesia</i> species, other than <i>B. microti</i>, in ixodid ticks collected from small mammals in the Republic of Korea

Kim, Tae Yun; Kim, Seong Yoon; Lee, Hee Il; Cho, Shin‐Hyeong; Lee, Wook-Gyo; Kim, Hyun Woo

doi:10.1002/vms3.581

Cited by 7 publications

(6 citation statements)

References 40 publications

(56 reference statements)

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“…Babesiosis caused by B. duncani is widespread in North America ( O'Connor et al., 2018 ; Scott and Scott, 2018 ; Swei et al., 2019 ), and epidemiological investigations found B. duncani in ticks in the northeast of China (accession no. KX008042, NCBI) and South Korea ( Kim et al., 2021 ). At the morphological level, there is no obvious difference between B. duncani and B. microti ( Wozniak et al., 1996 ; Conrad et al., 2006 ).…”

Section: Introductionmentioning

confidence: 99%

Establishment of a Transient and Stable Transfection System for Babesia duncani Using a Homologous Recombination Strategy

Wang

Chen

et al. 2022

Front. Cell. Infect. Microbiol.

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Genetic modification provides an invaluable molecular tool to dissect the biology and pathogenesis of pathogens. However, no report is available about the genetic modification of Babesia duncani, a pathogen responsible for human babesiosis that is widespread in North America, suggesting the necessity to develop a genetic manipulation method to improve the strategies for studying and understanding the biology of protozoan pathogens. The establishment of a genetic modification method requires promoters, selectable markers, and reporter genes. Here, the double-copy gene elongation factor-1α (ef-1α) and its promoters were amplified by conventional PCR and confirmed by sequencing. We established a transient transfection system by using the ef-1αB promoter and the reporter gene mCherry and achieved stable transfection through homologous recombination to integrate the selection marker hDHFR-eGFP into the parasite genome. The potential of this genetic modification method was tested by knocking out the thioredoxin peroxidase-1 (TPX-1) gene, and under the drug pressure of 5 nM WR99210, 96.3% of the parasites were observed to express green fluorescence protein (eGFP) by flow cytometry at day 7 post-transfection. Additionally, the clone line of the TPX-1 knockout parasite was successfully obtained by the limiting dilution method. This study provided a transfection method for B. duncani, which may facilitate gene function research and vaccine development of B. duncani.

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Section: Introductionmentioning

confidence: 99%

Establishment of a Transient and Stable Transfection System for Babesia duncani Using a Homologous Recombination Strategy

Wang

Chen

et al. 2022

Front. Cell. Infect. Microbiol.

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“…2020; Kim et al . 2021). Following centrifugation at 25,000 × g for 10 min, DNA was extracted from the supernatant of the homogenate using MagMAX® DNA Multi‐Sample Ultra 2.0 Kit (Applied Biosystems, Foster City, CA, USA) according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

“…For DNA extraction, individual ticks were homogenized in phosphate-buffered saline with ceramic (zirconium oxide) beads using a Precellys Evolution® homogenizer (Bertin Technologies, Bretonneux, France) as described previously (Kim et al 2020;Kim et al 2021). Following centrifugation at 25,000 ×g for 10 min, DNA was extracted from the supernatant of the homogenate using MagMAX® DNA Multi-Sample Ultra 2.0 Kit (Applied Biosystems, Foster City, CA, USA) according to the manufacturer's instructions.…”

Section: Dna Extraction From the Ticksmentioning

confidence: 99%

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Detection of US and Kobe‐type Babesia microti in ticks collected from small mammals of the Republic of Korea

Kim,

Seo

et al. 2024

Entomological Research

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Human babesiosis is an emerging tick‐borne zoonotic disease prevalent in large parts of the world. This study aimed to examine the status of Babesia microti infection in ticks collected from small mammals captured during the nationwide survey of the Republic of Korea (ROK), 2017. Ixodid ticks from small mammals of three species, Apodemus agrarius, Crocidura lasiura, and Tscherskia triton, were used for the molecular detection of B. microti. PCR analyses amplifying 18S ribosomal RNA (rRNA) of the genus Babesia showed 8.4% of the ticks to be positive. Nucleotide sequencing of the PCR products revealed two different genotypes of B. microti, the US and Kobe‐type. The US‐types of B. microti were detected in ticks from small mammals of almost all study sites, whereas detections of Kobe‐types were restricted to ticks from southern part of ROK. Phylogenetic analysis also indicated that each of the two types of B. microti clustered into two distinct clades. This is the first report on the presence of the US and Kobe‐type B. microti in ticks collected from small mammals trapped on a national scale in ROK.

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“…Hard ticks (Acari: Ixodidae) are hematophagous ectoparasites that can infest almost all vertebrates and transmit various diseases; as a result, ticks are regarded as one of the most important vectors of human and animal infections globally (Li et al 2020 ). Tick-borne diseases are spreading globally due to tick population growth, geographic expansion, and human travel and commerce (Gratz 2006 ; Kim et al 2021 ; Randolph and Rogers 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of Babesia microti in dromedary camels in Egypt

Ashour

Hamza

Kadry

et al. 2023

Trop Anim Health Prod

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Babesiamicroti (Apicomplexa: Piroplasmida) causes a medically important tick-borne zoonotic protozoan disease. Egyptian camels are susceptible to Babesia infection; however, just a few cases have been documented. This study aimed to identify Babesia species, specifically Babesia microti, and their genetic diversity in dromedary camels in Egypt and associated hard ticks. Blood and hard tick samples were taken from 133 infested dromedary camels slaughtered in Cairo and Giza abattoirs. The study was conducted from February to November 2021. The 18S rRNA gene was amplified by polymerase chain reaction (PCR) to identify Babesia species. Nested PCR targeting the β-tubulin gene was used to identify B. microti. The PCR results were confirmed by DNA sequencing. Phylogenetic analysis based on the ß-tubulin gene was used to detect and genotype B. microti. Three tick genera were identified in infested camels (Hyalomma, Rhipicephalus, and Amblyomma). Babesia species were detected in 3 out of 133 blood samples (2.3%), while Babesia spp. were not detected in hard ticks by using the 18S rRNA gene. B. microti was identified in 9 out of 133 blood samples (6.8%) and isolated from Rhipicephalus annulatus and Amblyomma cohaerens by the β-tubulin gene. The phylogenetic analysis of the β-tubulin gene revealed that USA-type B. microti was prevalent in Egyptian camels. The results of this study suggested that the Egyptian camels may be infected with Babesia spp. and the zoonotic B. microti strains, which pose a potential risk to public health.

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Molecular evidence of zoonotic Babesia species, other than B. microti, in ixodid ticks collected from small mammals in the Republic of Korea

Cited by 7 publications

References 40 publications

Establishment of a Transient and Stable Transfection System for Babesia duncani Using a Homologous Recombination Strategy

Establishment of a Transient and Stable Transfection System for Babesia duncani Using a Homologous Recombination Strategy

Detection of US and Kobe‐type Babesia microti in ticks collected from small mammals of the Republic of Korea

Molecular detection of Babesia microti in dromedary camels in Egypt

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