Molecular evidence of natural hybridization between Fasciola hepatica and F. gigantica

Agatsuma, Takeshi; Arakawa, Yohei; Iwagami, Moritoshi; Honzako, Y; Cahyaningsih, Umi; Kang, Sung-Soo; Hong, Sung-Jong

doi:10.1016/s1383-5769(00)00051-9

Cited by 117 publications

(100 citation statements)

References 17 publications

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“…This intermediate genotype has been also found in Korean Fasciola flukes using D2 sequences of 28S rDNA [2], in Chinese flukes using ITS2 [5] and in Japanese specimens using ITS1 and ITS2 [7]. Furthermore, two Korean flukes that were Kor1 in ITS1and Kor2 in NDI were also detected in this study.…”

Section: Discussionsupporting

confidence: 83%

“…their intermediate type as well as Japanese forms, although Agatsuma et al [2] reported that the mitochondrial NDI and COI sequences of 5 Korean Fasciola specimens were all F. gigantica-type. Furthermore, the NDI sequences of Korean haplotypes, Kor1 and Kor2a, were 100% identical to those of Fsp1 and Fsp2, respectively, which are major haplotypes of Japanese Fasciola forms.…”

Section: Discussionmentioning

confidence: 99%

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Molecular Characterization of Parthenogenic Fasciola sp. in Korea on the Basis of DNA Sequences of Ribosomal ITS1 and Mitochondrial NDI Gene

Itagaki

Kikawa

Terasaki

et al. 2005

The Journal of Veterinary Medical Science

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ABSTRACT. Nucleotide sequences of ribosomal internal transcribed spacer (ITS1) and mitochondrial NADH dehydrogenase I (NDI) gene were analyzed to genetically characterize aspermic Fasciola forms in Korea. From the difference in ITS1 sequences, Korean flukes were divided into 3 haplotypes represented by Kor1, Kor2 and Kor1/2, which had nucleotides identical to F. hepatica, F. gigantica and those overlapped between the two species, respectively. NDI sequences also showed that Korean flukes could be classified into 3 distinct haplotypes (Kor1: F. hepatica-type, . The sequences of Kor1 and Kor2a were 100% identical to those of the haplotypes Fsp1and Fsp2, respectively, which are major Fasciola forms in Japan. These findings strongly suggest that aspermic Fasciola forms in Korea and Japan originated from same ancestors and have recently spread throughout both countries. KEY WORDS: Fasciola sp., haplotype, ITS1, Korea, NDI.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Parthenogenic Fasciola sp. in Korea on the Basis of DNA Sequences of Ribosomal ITS1 and Mitochondrial NDI Gene

Itagaki

Kikawa

Terasaki

et al. 2005

The Journal of Veterinary Medical Science

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“…BLAST searches of the GenBank sequence database and comparisons with our own data clearly demonstrated that the flukes from the Vietnamese patients were pure Fasciola gigantica. It should be noted that hybrids between the two Fasciola species are known to exist in eastern Asia (1) and that their presence has recently been demonstrated in Vietnam by the use of molecular data (T. H. Le et al, data not shown). Hybrids inherit ITS2 sequences from both parents but inherit mitochondrial sequences only from the maternal species.…”

Section: Casementioning

confidence: 99%

Molecular Confirmation that Fasciola gigantica Can Undertake Aberrant Migrations in Human Hosts

Le¹,

Agatsuma³

et al. 2007

J Clin Microbiol

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“…Additionally, triploid F. hepatica has been found in Britain and Ireland (Fletcher et al, 2004). Recent molecular studies have suggested that the origin of Asian triploids may be hybridization between F. hepatica and F. gigantica (Itagaki & Tsutsumi, 1998;Agatsuma et al, 2000;Lin et al, 2007;Itagaki et al, 2009;Peng et al, 2009). Moreover, Terasaki et al (2000), Fletcher et al (2004) and Dreyfuss & Rondelaud (2008) suggest that triploidy in Fasciola spp.…”

Section: Discussionmentioning

confidence: 99%

A description of karyotype of the giant liver fluke Fascioloides magna (Trematoda, Platyhelminthes) and a review of Fasciolidae cytogenetics

Reblánová

Špakulová

Orosová³

et al. 2010

Helminthologia

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69 SummaryThe study describes a karyotype of a common parasite of cervids, the giant liver fluke, Fascioloides magna (Trematoda, Platyhelminthes). The chromosome set of F. magna comprises 11 pairs of chromosomes, all classified as subtelocentric except for the submeta-metacentric pair No. 8 and the submetacentric pair No. 10 (2n = 22, n = 1sm + 1sm-m + 9st). The first longest pair is 4.65 μm long and the length decreases continuously to the 1.92 μm length of the last pair No. 11. No distinct secondary constriction has been observed in mitotic preparations. Fluorescent DAPIstaining reveals distinct heterochromatin bands on all 11 chromosome pairs in the centromeric regions; another DAPI-positive bands are localized at the end of the long arms of chromosomes No. 5 and the last less distinct signals appear interstitially on the long arms of the pair No. 6. Synchronous meiotic divisions of 8-spermatocyte groups have been observed during spermatogenesis, similarly with a development of spermatocytes in other trematodes. In the first two stages of heterotypic spermatocyte division, 11 bivalents (n = 11) are regularly observed, confirming the diploid number of 22 elements. Furthermore, the present analysis summarises and discusses available cytogenetic data on Fasciolidae flukes suitable for future studies on taxonomy or phylogenetic interrelationships within the family.

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Molecular evidence of natural hybridization between Fasciola hepatica and F. gigantica

Cited by 117 publications

References 17 publications

Molecular Characterization of Parthenogenic Fasciola sp. in Korea on the Basis of DNA Sequences of Ribosomal ITS1 and Mitochondrial NDI Gene

Molecular Characterization of Parthenogenic Fasciola sp. in Korea on the Basis of DNA Sequences of Ribosomal ITS1 and Mitochondrial NDI Gene

Molecular Confirmation that Fasciola gigantica Can Undertake Aberrant Migrations in Human Hosts

A description of karyotype of the giant liver fluke Fascioloides magna (Trematoda, Platyhelminthes) and a review of Fasciolidae cytogenetics

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