Molecular Evidence of Infections with<i>Babesia gibsoni</i>Parasites in Japan and Evaluation of the Diagnostic Potential of a Loop-Mediated Isothermal Amplification Method

Ikadai, Hiromi; Tanaka, Hiroko; Shibahara, Nona; Matsuu, Aya; Uechi, Masami; Itoh, Naoyuki; Oshiro, Sugao; Kudo, Noboru; Igarashi, Ikuo; Oyamada, Takashi

doi:10.1128/jcm.42.6.2465-2469.2004

Cited by 104 publications

(57 citation statements)

References 16 publications

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“…All positive serum samples from domestic dogs both from Japan and China were IFAT-positive (data not shown). The present data of prevalence of canine B. gibsoni infection in western Japan is within the range reported previously [10,11,18]. In addition, to our knowledge, this is the first report describing a survey on canine piroplasmosis in Nanjing, China.…”

Section: Discussionsupporting

confidence: 63%

Serodiagnosis of Babesia gibsoni Infection in Dogs by an Improved Enzyme-Linked Immunosorbent Assay with Recombinant Truncated P50

Verdida

Hara

Xuan

et al. 2004

The Journal of Veterinary Medical Science

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ABSTRACT. The surface antigen P50 of Babesia gibsoni is an important candidate for the development of a diagnostic reagent for canine piroplasmosis. In order to establish an effective diagnostic method for practical use, the gene encoding truncated P50 (P50t) lacking a signal peptide and C-terminal hydrophobic regions were cloned and expressed in Escherichia coli as a fusion protein with glutathione S-transferase (GST). More than 90% portion of the GST-P50t was expressed as a soluble form, in contrast with GST-P50f (full-len gth), which was completely expressed as an insoluble form. This result indicates that removal of the hydrophobic signal peptide and C-terminus had dramatically improved its hydrophilicity. The purified GST-P50t was tested in an enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to B. gibsoni in dogs. The ELISA with GST-P50t clearly differentiated between B. gibsoni-infected dog sera and uninfected dog sera. In addition, the ELISA detected no cross-reactivity with sera from dogs experimentally infected with the closely related parasites, B. canis canis, B. canis vogeli, and B. canis rossi. Field serum samples collected from dogs in Japan and China were examined for the diagnosis of B. gibsoni infection by using the ELISA. 14.5% (9/62), 5.8% (7/120), and 5.4% (2/37) of tested samples were positive for dogs from Okinawa, Yamaguchi, and Osaka prefectures, Japan, respectively. On the other hand, 4.8% (2/41) of tested samples were positive for dogs from Nanjing, China. These results suggest that the GST-P50t could be a reliabl e reagent for practical use in ELISA for the serodiagnosis of canine piroplasmosis caused by B. gibsoni.

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Section: Discussionsupporting

confidence: 63%

Serodiagnosis of Babesia gibsoni Infection in Dogs by an Improved Enzyme-Linked Immunosorbent Assay with Recombinant Truncated P50

Verdida

Hara

Xuan

et al. 2004

The Journal of Veterinary Medical Science

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“…Haemaphysalis species has an especially wide geographical distribution, including Aomori Prefecture [23]; therefore, the vector ticks might have contributed to the spread of B. gibsoni infection among Tosa dogs, but dogs with acute B. gibsoni infection were not recognized in dogs of any other breed. Furthermore, natural infection with this pathogen, as identified by PCR assays, has been found only in dogs of the Tosa breed, and has not been identified in tests of 872 dogs of other breeds in dogs of any other breeds in Aomori Prefecture [11]. It has been reported in 2003, that B. gibsoni parasite was not detected in ticks from eastern Japan, including Aomori Prefecture, in an epidemiological survey [12].…”

Section: Discussionmentioning

confidence: 99%

Incidence of Canine Babesia gibsoni Infection and Subclinical Infection among Tosa dogs in Aomori Prefecture, Japan

Matsuu

Kawabe

Koshida

et al. 2004

The Journal of Veterinary Medical Science

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ABSTRACT. To identify the incidence of Babesia gibsoni (B. gibsoni) in Aomori Prefecture, northeastern Japan, dogs with acute B. gibsoni infection were investigated at the Animal Teaching Hospital, Kitasato University, between April 2002 and March 2003. Eighteen dogs with acute B. gibsoni infection were recognized; they were all male dogs of the fighting dog breed Tosa. Their platelet counts were below normal and their packed cell volumes (PCVs) were at various levels. We collected blood samples from 141 Tosa dogs from Aomori Prefecture and used polymerase chain reaction assay to investigate the incidence of subclinical B. gibsoni infection. We also looked into the serological abnormalities associated with thrombocytopenia or anemia in subclinical infection. Forty-one of 87 dogs (47.1%) with histories of dog fighting, and one dog of 54 without a history of dog fighting were positive for B. gibsoni; that is, 42 of 141 dogs (29.8%) showed a positive result. The mean platelet counts of dogs with subclinical infection were significantly lower and levels of anti-platelet IgG were significantly higher than levels for dogs without infection. Anti-erythrocyte membrane IgG levels were significantly higher in dogs with subclinical infections, although mean PCVs were not significantly different. Tosa dogs from Aomori Prefecture, Japan, were highly infected with B. gibsoni subclinically and this pathogen might be successfully transmitted during dog fighting. Dogs with subclinical infections were at risk of chronic thrombocytopenia, which may be due to autoimmune mechanisms. KEY WORDS: anti-erythrocyte membrane IgG, anti-platelet IgG, Babesia gibsoni, Tosa dog.

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“…This technique has been proven to be an accurate, rapid and simple method, which amplifies the target nucleic acid under isothermal conditions (Notomi et al, 2000). Recently, wide applicability of LAMP in the detection of parasitic protozoa such as Babesia, Plasmodium, Leishmania and Trypanosoma in clinical samples has been demonstrated (Ikadai et al, 2004;Poon et al, 2006;Njiru et al, 2008;Takagi et al, 2009;Thekisoe et al, 2007;Laohasinnarong et al, 2011). Studies have also shown the application of LAMP to survey vectors of infectious diseases (Aonuma et al, 2009;Thekisoe et al, 2010;Nakao et al, 2010); however, these studies used purified DNA as a template for the LAMP assays.…”

Section: Introductionmentioning

confidence: 99%

Development of a loop-mediated isothermal amplification method for rapid mass-screening of sand flies for Leishmania infection

et al. 2014

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Entomological monitoring of Leishmania infection in leishmaniasis endemic areas offers epidemiologic advantages for predicting the risk and expansion of the disease, as well as evaluation of the effectiveness of control programs. In this study, we developed a highly sensitive loop-mediated isothermal amplification (LAMP) method for the mass screening of sand flies for Leishmania infection based on the 18S rRNA gene. The LAMP technique could detect 0.01 parasites, which was more sensitive than classical PCR. The method was robust and could amplify the target DNA within 1 hr from a crude sand fly template without DNA purification. Amplicon

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Molecular Evidence of Infections withBabesia gibsoniParasites in Japan and Evaluation of the Diagnostic Potential of a Loop-Mediated Isothermal Amplification Method

Abstract: Detection and analysis of

Cited by 104 publications

References 16 publications

Serodiagnosis of Babesia gibsoni Infection in Dogs by an Improved Enzyme-Linked Immunosorbent Assay with Recombinant Truncated P50

Serodiagnosis of Babesia gibsoni Infection in Dogs by an Improved Enzyme-Linked Immunosorbent Assay with Recombinant Truncated P50

Incidence of Canine Babesia gibsoni Infection and Subclinical Infection among Tosa dogs in Aomori Prefecture, Japan

Development of a loop-mediated isothermal amplification method for rapid mass-screening of sand flies for Leishmania infection

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