Molecular evidence of Erysiphe pisi on pea and E. trifoliorum on white clover in northeast India

Baiswar, Pankaj; Ngachan, S. V.; Verma, V. K.; Kumar, Rajesh; Jha, Ashish; Chandra, Sulekh

doi:10.1007/s13314-015-0165-2

Cited by 8 publications

(7 citation statements)

References 5 publications

(7 reference statements)

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“…The variation between individual rDNA repeats can occasionally be observed amongst the ITS and IGS regions. The amplicon size of the 18s rRNA region-based primers, viz ., ITS 1 and 4, PMITS 1 and 2, and EryF and EryR on E. pisi consistent with the reported nucleotide size of E. pisi , from central and northeast India [ 8 ]. These findings confirmed the identity of all of the isolates investigated.…”

Section: Resultssupporting

confidence: 87%

“…Pea powdery mildew is an airborne fungal disease caused by Erysiphe pisi DC (Order: Erysiphales ), a filamentous fungus responsible for 25–70% of yield losses in pea cultivation [ 7 , 8 ]. Recently, the authorized Global Biodiversity Information Facility (GBIF), Denmark, proposed the preferred name of the pathogen as Erysiphe pisi var.…”

Section: Introductionmentioning

confidence: 99%

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Genetic Diversity Analysis based on the Virulence, Physiology and Regional Variability in Different Isolates of Powdery Mildew in Pea

Seethapathy

Sankaralingam²,

Pandita³

et al. 2022

JoF

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Powdery mildew is an omnipresent disease that reduces the yield and quality of pea crops (Pisum sativum L.). To examine the powdery mildew pathogen’s morphological, molecular, and genetic diversity, we collected samples of powdery mildew-affected pea crops from ten distinct locations in the Nilgiris district of Tamil Nadu, India. The pathogen Erysiphe pisi was identified morphologically based on anamorphic characters. Molecular identification of E. pisi isolates was befitted by targeting the internal transcribed spacer (ITS) region of rDNA and specific primers of powdery mildew fungi. The genetic variation between ten different E. pisi isolates collected from topographically distinct mountainous areas was studied using random amplified polymorphic (RAPD). Based on its morphological characteristics, the powdery mildew fungus presented high similarities to E. pisi. Molecular characterization of the ITS rDNA of E. pisi produced 650 bp nucleotides, PMITS (powdery mildew-internal transcribed region) primers produced 700 bp nucleotides, and an Erysiphe specific ITS primer pair amplified and synthesized 560 bp nucleotides. According to the findings, the collected E. pisi strains exhibited a low level of genetic diversity and only a slight differential in virulence on the host. In the study, E. pisi isolates from Anumapuram, Emerald Valley, Indira Nagar, and Thuneri showed a greater disease incidence in the natural field conditions and shared the same genetic lineage with other isolates in UPGMA hierarchical cluster analysis based on RAPD markers. There was no evidence of a link between the occurrence of the disease and these grouped populations.

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Section: Resultssupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Genetic Diversity Analysis based on the Virulence, Physiology and Regional Variability in Different Isolates of Powdery Mildew in Pea

Seethapathy

Sankaralingam²,

Pandita³

et al. 2022

JoF

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“…Knowing the importance of this vegetable crop by exploring the literature we, therefore, continued our investigation by determining the diversity in pathogen-resistant pea lines at er loci 15 , which were screened in vitro 16 . Collection and DNA profiling of powdery mildew causing pea pathogen 17 , 18 corresponds to genus Erysiphe with species, pisi and trifol i, respectively 7 , 19 , 20 . The North-Western Himalayan region is the most prevalent hot spot of powdery mildew 16 .…”

Section: Discussionmentioning

confidence: 99%

Deciphering diversity at er loci for diversification of powdery mildew resistance in pea

Banyal

Dixit

Chaudhary³

et al. 2022

Sci Rep

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Agricultural biotechnology aims to scrutinize the field crops which feed half of the world’s population by improving their agronomic traits using various biotechnological tools. Pea- an important cash crop, rich in nutrients, but frequently infected with powdery mildew (fungal disease caused by Erysiphe pisi) that destroys the whole crop and causes economic loss for growers. We, therefore, targeted this research to find the pathogen-resistant pea lines and further decipher the diversity at er locus among resistant pea lines. Screening for resistant pea lines was done with Erysiphe pisi isolates (Genebank submission: KX455922.1) under the net house and greenhouse conditions. Molecular studies revealed that the Erysiphe resistant (er1) gene was present in 40 lines out of selected 50 pea lines and the mutational character was conferred up to 36 genotypes with 11 haplotype groups. The haplotype (gene) diversity (Hd) was found to be 0.5571 ± 0.099 SD and the nucleotide diversity (Pi) was 0.0160 ± 0.0042 SD Majority of resistant lines (67%) occurred in Hap-1, other remaining haplotypes (Hap 2–10) having 33% resistant lines, each showing characteristic nucleotide substitutions with respect to reference PsMLO1 gene; genotypes from these divergent haplotypes can be used in pea resistance breeding to avoid genetic homogeneity and genetic vulnerability.

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“…Pathogen causing powdery mildew was collected from Northwest Himalayas in which the maximum number of isolates were collected from Lahul area of trans-Himalayas [14] [15]. Molecular and morphological identi cation of test pathogen (from pea leaf and pea clover) corresponds to genus Erysiphe with species pisi and trifoli, respectively [16] [7] [17]. Screening was done under net-house and green house conditions where description of infection was recorded [6].…”

Section: Discussionmentioning

confidence: 99%

Deciphering Diversity at er Loci for Diversification of Powdery Mildew Resistance in Pea

Banyal

Dixit

Chaudhary

et al. 2022

Preprint

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Agricultural biotechnology aims to scrutinize the field crops which feed amongst half of the world’s population by improving their agronomic traits using various biotechnological tools. Pea- an important cash crop, rich in nutrients, but frequently infected with powdery mildew (fungal disease) that destroy the whole crop which causes the economy loss for growers. We therefore, targeted this research to find the pathogen resistant pea lines and further decipher the diversity at er locus among resistant pea lines. Screening for resistant pea lines was done with Erysiphe pisi isolates (Genebank submission: KX45 5922.1) under net house and green house conditions. Molecular studies revealed that Erysiphe resistant (er1) gene was present in 40 lines out of selected 50 pea lines and the mutational character was conferred up to 36 genotypes with 11 haplotype groups. The haplotype (gene) diversity (Hd) was found to be 0.5571±0.099 SD and the nucleotide diversity (Pi) was 0.0160±0.0042 SD Majority of resistant lines (67%) occurred in Hap-1, other remaining haplotypes (Hap 2-10) having 33% resistant lines, each showing characteristic nucleotide substitutions with respect to reference PsMLO1 gene; genotypes from these divergent haplotypes can be used in pea resistance breeding to avoid genetic homogeneity and genetic vulnerability.

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Molecular evidence of Erysiphe pisi on pea and E. trifoliorum on white clover in northeast India

Cited by 8 publications

References 5 publications

Genetic Diversity Analysis based on the Virulence, Physiology and Regional Variability in Different Isolates of Powdery Mildew in Pea

Genetic Diversity Analysis based on the Virulence, Physiology and Regional Variability in Different Isolates of Powdery Mildew in Pea

Deciphering diversity at er loci for diversification of powdery mildew resistance in pea

Deciphering Diversity at er Loci for Diversification of Powdery Mildew Resistance in Pea

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