Molecular evidence of bacteria in Melophagus ovinus sheep keds and Hippobosca equina forest flies collected from sheep and horses in northeastern Algeria

Boucheikhchoukh, Mehdi; Mechouk, Noureddine; Benakhla, Ahmed; Raoult, Didier; Parola, Philippe

doi:10.1016/j.cimid.2019.05.010

Cited by 17 publications

(23 citation statements)

References 57 publications

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“…Kowal and his colleagues [86] reported the role of Hippoboscids in the transmission of bacterial pathogens such as Anaplasma and Bartonella. Moreover, Boucheikhchoukh and his colleagues [87] detected Bartonella and Wolbachia sp. in H. equina.…”

Section: Discussionmentioning

confidence: 98%

Molecular Characterization of Some Equine Vector-Borne Pathogens and Identification of Their Vectors in Egypt

Abdullah¹,

Aboelsoued²,

Farag³

et al. 2020

Preprint

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Background Equine vector borne diseases (EVBD) have been considered emerging and reemerging diseases transmitted by arthropods and most of these diseases have zoonotic concern. This study was designed to screen EVBD in equines and their vectors using molecular analyses and identify vectors by MALDI-TOF and molecular techniques.Methods A total of 335 blood samples were collected from apparently healthy equines (320 from horses and 15 from donkeys) from Cairo and Beni-Suef provinces in Egypt. A total of 166 arthropods (105 sucking flies and 61 ticks) were collected from the same animals. MALDI-TOF and molecular techniques were used to confirm the findings of morphological identification of vector. Quantitative PCR and Standard PCR coupled with sequencing were performed in equines and vectors DNA for screening multiple pathogens.Results MALDI-TOF and molecular techniques confirmed that Hippoposca equina (louse fly), Rhipicephalus annulatus (Rh. annulatus) and Rh. microplus ixodid ticks were found. In vectors, we identified Anaplasma marginale (A. marginale; 1.6%), A. platys-like (1.6%) and a new Ehrlichia sp. (4.9%) in Rh. microplus, while Ehrlichia rustica (E. rustica) was found in Rh. microplus and Rh. annulatus. Likewise, Borrelia theileri was identified in Rh. microplus (3.3%). For H. equina, Anaplasma and Borrelia sp. DNA were detected by qPCR only. In equines, A. marginale (0.6%), A. ovis (0.6%) and Theileria ovis (T. ovis; 0.6%) were found in donkeys. In horses, T. equi (1.2%) and a new Theileria sp. Africa (2.7%) were identified.Conclusions For the first time, we reported here the presence of Rh. microplus as a competent tick for Rh. annulatus in Egypt using MALDI-TOF and molecular identification. To the best of our knowledge, we provided the first detection of different pathogens as A. marginale, A. platys-like, E. rustica, new Ehrlichia sp., B. theileri in Rh. microplus, A. marginale, A. ovis and T. ovis in donkeys and a new Theileria sp. Africa in horses in Egypt.

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Section: Discussionmentioning

confidence: 98%

Molecular Characterization of Some Equine Vector-Borne Pathogens and Identification of Their Vectors in Egypt

Abdullah¹,

Aboelsoued²,

Farag³

et al. 2020

Preprint

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“…Another investigation of Bartonella in ticks from different countries reported positive rates of 1.05% (2/191) in Lithuania ( 44 ), 6.86% (19/277) in Algeria ( 9 ), 10.31% (13/126) in Brazil ( 12 ), 18.04% (57/316) in Poland ( 45 ), 10.4% (13/125) and 15.7% (40/254) in the Brazilian Cerrado and Taiwan ( 11 , 12 ), 4.0% (8/200) in Malaysia ( 14 ), 5.8% (5/85) and 2.5% (1/40) in Korea ( 15 ), 8.56% (25/292) in North Korea ( 16 ), and 0.4% (1/234) in Pakistan ( 17 ). Previous studies on Bartonella in other ruminant-related blood-eating arthropods showed that the positive rates were 78.79% (26/33) in Hippoboscidae flies from Algeria ( 9 ) 4.21% (4/95) and 84.62% (11/13) in H. tuberculatus from the Brazilian Cerrado and H. quadripertusus from Israel ( 12 , 13 ), 2.93% (16/546) in C. felis from Tunisia ( 18 ), 100% (133/133) and 36.87% (104/282) in M. ovinus from Central Europe and northeastern Algeria ( 19 , 20 ), and 84.75% (50/59) and 93.75% (45/48) in L. cervi from Norway and Europe ( 6 , 8 ). There were differences in the positive rates of Bartonella in ticks from different countries and regions, which may be related to the location, number, detection methods and ecological environment of the collected samples.…”

Section: Discussionmentioning

confidence: 99%

“…Forty-two species of ticks belonging to nine genera were reported in Xinjiang, accounting for more than 1/3 of the total number of tick species in China ( 26 , 46 , 47 ); the wide distribution of ticks has a significant impact on the development of animal husbandry and on public health. In recent years, B. melophagi was detected in M. ovinus from northeastern Algeria ( 20 ), China ( 31 ), central Europe ( 19 ), the western United States ( 48 ), and northern Oromia, Ethiopia ( 12 ). B. melophagi was detected in white-tailed deer ( 49 ) and human blood ( 50 ) in the United States.…”

Section: Discussionmentioning

confidence: 99%

“…and Haematobia spp. from California ( 10 ), Rhipicephalus microplus from Brazilian Cerrado and Taiwan ( 11 , 12 ), Haematopinus tuberculatus from Brazilian Cerrado and H. quadripertusus from Israel ( 12 , 13 ), Haemaphysalis bispinosa from Malaysia ( 14 ), H. flava and Ixodes persulcatus from Korean ( 15 ), H. longicornis from North Korea and Korean ( 15 , 16 ), Hyalomma anatolicum from Pakistan ( 17 ), Ctenocephalides felis from Tunisia ( 18 ), sheep keds ( Melophagus ovinus ) from Central Europe and northeastern Algeria ( 19 , 20 ), and deer keds ( Lipoptena cervi ) from Norway ( 6 ).…”

Section: Introductionmentioning

confidence: 99%

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Molecular Evidence of Bartonella melophagi in Ticks in Border Areas of Xinjiang, China

Ren

Lin

et al. 2021

Front. Vet. Sci.

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Bartonella are gram-negative intracellular bacteria; certain species of Bartonella can cause diseases in mammals and humans. Ticks play a major role in the transmission of Bartonella. Xinjiang is the largest province in China according to land area and has one-third of the tick species in China; the infection rate of Bartonella in ticks in the Xinjiang border areas has not been studied in detail. Therefore, this study investigated tick infections by Bartonella in Xinjiang border areas, and the purpose of the study was to fill in gaps in information regarding the genetic diversity of tick infections by Bartonella in Xinjiang. We tested 1,549 tick samples from domestic animals (sheep and cattle) for Bartonella using ribC-PCR. Positive samples from the ribC-PCR assay for Bartonella spp. were further subjected to PCR assays targeting the ITS, rpoB and gltA genes followed by phylogenetic analyses. Bartonella DNA was detected in 2.19% (34/1,549) of tick samples, and the ITS, rpoB and gltA genes of ribC gene-positive samples were amplified to identify nine samples of Bartonella melophagi. In this study, molecular analysis was used to assess the presence and genetic diversity of B. melophagi in ticks collected from sheep and cattle from Xinjiang, China. This study provides new information on the presence and identity of B. melophagi in ticks from sheep and cattle.

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“…It mainly occurs in sheep, dogs, horses, etc., resulting in animal emaciation, anemia, poor growth, decreased quality of livestock products, and even death of individual animal pups. Besides, the most serious harm of Melophagus ovinus is that it can be used as a vector to spread bluetongue virus ( 1 ), Trypanosoma ( 2 ), Bartonella ( 3 ), Rickettsia ( 4 ), Acinetobacter ( 5 ), and other pathogens. Among them, bluetongue is one of the most susceptible sheep infectious diseases, with an incidence rate of 76% ( 6 ), causing great harm to animal husbandry.…”

Section: Introductionmentioning

confidence: 99%

Tandem mass tag labeled quantitative proteomic analysis of differential protein expression on total alkaloid of Aconitum flavum Hand.-Mazz. against melophagus ovinus

et al. 2022

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Melophagus ovinus disease is a common ectoparasitosis, which can lead to a decrease in animal production performance, product quality, and even death. Aconitum flavum Hand.-Mazz. has many pharmacological activities including insecticidal, heat-clearing, analgesic, and dehumidifying. However, there are few researches focused on the effects and related mechanism of Aconitum flavum Hand.-Mazz. in killing Melophagus ovinus. In this study, 11 alkaloids of Aconitum flavum Hand.-Mazz. were detected, and its total alkaloid activity was determined. The results showed when the total alkaloid concentration was 64 mg/ml and the treatment time was 16 h, the killing rate of Melophagus ovinus reached 100%. Through the observation of the differences in the surface of Melophagus ovinus in each experimental group, it was found that the morphology of the posterior end of the female Melophagus ovinus in the alkaloid treatment group was significantly different from that of the blank and positive control groups, and most of the epidermal tissue was obsessive and missing. Moreover, the enzyme activity determination results of 64 mg/ml group were significantly different when compared with the normal control group, while there was no significant difference in other groups. Then, the Melophagus ovinus gene library was established by the unreferenced genome transcriptome sequencing, the proteomic comparison was performed using tandem mass tag labeled protein detection technology, and finally, the samples were quantitatively analyzed by liquid chromatography-mass spectrometry tandem and bioinformatics methods. Based on the above experimental results, it was speculated that Aconitum flavum Hand.-Mazz. total alkaloids may cause the imbalance of protein disulfide isomerase expressions by affecting the regulation of Hsp40 cellular protein homeostasis and the oxidation of protein disulfide isomerase and related proteins. This would affect the selective recognition of signal sequence, the targeted transport of Sec 61, and the correct folding of the three-dimensional structure of amino acid chain, weakening the clearance of amino acid chains that cannot be correctly folded and eventually resulting in the killing of Melophagus ovinus. This study preliminarily revealed the mechanism of Aconitum flavum Hand.-Mazz. total alkaloids against Melophagus ovinus and provided a theoretical basis for the screening of Melophagus ovinus action targets and the development of new veterinary drugs.

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Molecular evidence of bacteria in Melophagus ovinus sheep keds and Hippobosca equina forest flies collected from sheep and horses in northeastern Algeria

Cited by 17 publications

References 57 publications

Molecular Characterization of Some Equine Vector-Borne Pathogens and Identification of Their Vectors in Egypt

Molecular Characterization of Some Equine Vector-Borne Pathogens and Identification of Their Vectors in Egypt

Molecular Evidence of Bartonella melophagi in Ticks in Border Areas of Xinjiang, China

Tandem mass tag labeled quantitative proteomic analysis of differential protein expression on total alkaloid of Aconitum flavum Hand.-Mazz. against melophagus ovinus

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