Molecular evidence for the presence of chlamydia in the synovium of patients with reiter's syndrome

Rahman, Mahboob U.; Cheema,; Schumacher, H. Ralph; Hudson, Alan P.

doi:10.1002/art.1780350506

Cited by 129 publications

(45 citation statements)

References 33 publications

(24 reference statements)

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“…Nucleic acids were extracted from biopsy samples and SF pellets as previously described (6). Briefly, frozen samples were homogenized in a Dounce containing 0.5 ml of buffer (25 mM Tris, pH 8, 10 mM EDTA, 100 m M NaC1,0.5% sodium dodecyl sulfate) and 1 ml of buffered phenol at 65°C.…”

Section: Methodsmentioning

confidence: 99%

“…Importantly, an infectious etiology for some forms of reactive arthritis (ReA) has long been postulated, and C trachomatis has emerged as a prominent agent among organisms associated with the disease (43). Despite evidence linking ReA with chlamydial infection, however, it is not clear whether the process leading to the disease involves viable bacteria in the synovium at some point or whether the disease is purely immune-mediated in response to chlamydial antigenic debris at the synovial site (5)(6)(7).…”

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confidence: 99%

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Comparison of synovial tissue and synovial fluid as the source of nucleic acids for detection of Chlamydia trachomatis by polymerase chain reaction

Branigan

Gérard

Hudson

et al. 1996

Arthritis & Rheumatism

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Objective. Difficulties in detecting Chlamydia trachomatis in human joints by polymerase chain reaction (PCR) may be related to whether synovial tissue or synovial fluid (SF) is used as the source of DNA in PCR amplification. In this study, a new PCR assay was developed and used to compare chlamydial DNA in paired samples of SF and synovial tissue from patients with arthritis.Methods. The PCR assay targeted the ribosomal RNA operons, which are present in 2 copies on the C trachomatis chromosome. DNA from several relevant bacteria and chlamydial serovars was used for testing this screening system. The detection of chlamydial DNA in nucleic acid preparations from matched samples of SF and synovial tissue was compared by PCR assay. Samples were obtained from 55 patients, including patients with reactive arthritis, Reiter's syndrome, and other arthropathies.Results. Testing of the PCR screening system confirmed it to be highly specific and sensitive. Use of this assay to screen DNA from SF and synovial tissue samples showed that 29 (53%) of 55 synovial tissue preparations were positive for chlamydial DNA, but only 16 (29%) of the matched SF samples from these 29 patients were similarly positive. Five (9%) of 55 SF samples, but not their tissue counterparts, were positive for chlamydial DNA by PCR.Conclusion. Detection of chlamydial DNA in the joints of patients by PCR gives positive results more often when synovial tissue rather than SF is the source of target nucleic acids. Although synovial tissue is the source of choice for the most reliable determination of chlamydia in the joint, both synovial tissue and SF should be assayed if possible.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Comparison of synovial tissue and synovial fluid as the source of nucleic acids for detection of Chlamydia trachomatis by polymerase chain reaction

Branigan

Gérard

Hudson

et al. 1996

Arthritis & Rheumatism

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“…Chlamydia1 hsp57 has been identified as the immunodominant protein for a CD4 T cell response of guinea pigs after infection of the eye, which leads to hypersensitivity to chlamydial antigen (23), a situation resembling that in reactive arthritis (47). Furthermore, Chlamydia-derived hsp57 peptides that are able to bind to HLA-B27 have recently been characterized (48).…”

Section: Discussionmentioning

confidence: 99%

Recognition of chlamydial antigen by HLA‐B27‐restricted cytotoxic T cells in HLA‐B*2705 transgenic CBA (H‐2^k) mice

Kuon

Lauster

Böttcher

et al. 1997

Arthritis & Rheumatism

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Objective. The association of reactive arthritis (ReA) with HLA‐B27 and the presence of bacterial antigen in joints with ReA suggest that bacterial peptides might be presented by the HLA‐B27 molecule and thus stimulate CD8 T cells. This study was performed to investigate the B27‐restricted cytotoxic T lymphocyte (CTL) response to Chlamydia trachomatis, using the model of HLA‐B27 transgenic mice. Methods. CBA (H‐2k) mice homozygous for HLA‐B*2705 and human β2‐microglobulin expression were immunized with C trachomatis or with the chlamydial 57‐kd heat‐shock protein (hsp57) coupled to latex beads. Cytotoxicity of lymphocytes from in vivo—primed transgenic mice was tested against C trachomatis—infected targets. Blocking experiments were performed with monoclonal antibodies (MAb) against class I major histocompatibility complex molecules. Results. A Chlamydia‐specific lysis of both B27‐transfected and nontransfected target cells was observed. This response could be inhibited by anti‐B27 and anti‐H2 MAb. CTL from mice immunized with hsp57 were not able to lyse Chlamydia‐infected target cells, and Chlamydia‐specific CTL could not destroy targets loaded with hsp57. Conclusion. These results suggest the existence of at least 2 CTL populations in this mouse model: one recognizing peptide of bacteria‐infected cells restricted by HLA‐B*2705 and the other recognizing peptide of bacteria‐infected cells restricted by the murine H‐2Kk molecule. It does not appear that hsp57 is a major target for the CD8 T cell response directed against Chlamydia. This animal model opens the way for identifying bacterial epitopes presented by HLA‐B27, and might thus help to clarify the pathogenesis of B27‐associated diseases.

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“…(10), however, these recurrences are generally not related to overt genitourinary infections (11). It was also reported that about 43% of RS patients with recurrent attacks showed no discriminate urethritis infection (12 (13). The nature of such persistent chlamydial infection was studied by several groups (14,15 …”

Section: F I G U R E 3 T H E C L I N I C a L C O U R S E O F T H E mentioning

confidence: 99%

Reactive Arthritis which Occurred One Year After Acute Chlamydial Urethritis

et al. 2008

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Molecular evidence for the presence of chlamydia in the synovium of patients with reiter's syndrome

Cited by 129 publications

References 33 publications

Comparison of synovial tissue and synovial fluid as the source of nucleic acids for detection of Chlamydia trachomatis by polymerase chain reaction

Comparison of synovial tissue and synovial fluid as the source of nucleic acids for detection of Chlamydia trachomatis by polymerase chain reaction

Recognition of chlamydial antigen by HLA‐B27‐restricted cytotoxic T cells in HLA‐B*2705 transgenic CBA (H‐2^k) mice

Reactive Arthritis which Occurred One Year After Acute Chlamydial Urethritis

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Molecular evidence for the presence of chlamydia in the synovium of patients with reiter's syndrome

Cited by 129 publications

References 33 publications

Comparison of synovial tissue and synovial fluid as the source of nucleic acids for detection of Chlamydia trachomatis by polymerase chain reaction

Comparison of synovial tissue and synovial fluid as the source of nucleic acids for detection of Chlamydia trachomatis by polymerase chain reaction

Recognition of chlamydial antigen by HLA‐B27‐restricted cytotoxic T cells in HLA‐B*2705 transgenic CBA (H‐2k) mice

Reactive Arthritis which Occurred One Year After Acute Chlamydial Urethritis

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Recognition of chlamydial antigen by HLA‐B27‐restricted cytotoxic T cells in HLA‐B*2705 transgenic CBA (H‐2^k) mice