Molecular Evidence for Multiple Infections as Revealed by Typing of
            <i>Asaia</i>
            Bacterial Symbionts of Four Mosquito Species

Chouaia, Bessem; Rossi, Paolo; Montagna, Matteo; Ricci, Irene; Crotti, Elena; Damiani, Claudia; Epis, Sara; Faye, Ingrid; Sagnon, N’Falé; Alma, Alberto; Favia, Guido; Daffonchio, Daniele; Bandi, Claudio

doi:10.1128/aem.01747-10

Cited by 83 publications

(78 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In mosquitoes of the genus Anopheles , Asaia has been shown to infect through both per-oral [6,19] and venereal routes, from male to female, followed in each case by vertical spread from the mother to the offspring [5,20]. These transmission routes are in agreement with both the incongruent evolutionary history of Asaia and its host species, and with the high frequency of infections with multiple Asaia strains in mosquitoes [21]. However, very little is known about the rate and mechanisms of horizontal transfer of Asaia in hemipterans like S. titanus .…”

Section: Introductionmentioning

confidence: 85%

Horizontal transmission of the symbiotic bacterium Asaia sp. in the leafhopper Scaphoideus titanus Ball (Hemiptera: Cicadellidae)

et al. 2012

Self Cite

View full text Add to dashboard Cite

BackgroundBacteria of the genus Asaia have been recently recognized as secondary symbionts of different sugar-feeding insects, including the leafhopper Scaphoideus titanus, vector of Flavescence dorée phytoplasmas. Asaia has been shown to be localized in S. titanus gut, salivary glands and gonoducts and to be maternally transmitted to the progeny by an egg smearing mechanism. It is currently not known whether Asaia in S. titanus is transmitted by additional routes. We performed a study to evaluate if Asaia infection is capable of horizontal transmission via co-feeding and venereal routes.ResultsA Gfp-tagged strain of Asaia was provided to S. titanus individuals to trace the transmission pathways of the symbiotic bacterium. Co-feeding trials showed a regular transfer of bacterial cells from donors to recipients, with a peak of frequency after 72 hours of exposure, and with concentrations of the administrated strain growing over time. Venereal transmission experiments were first carried out using infected males paired with uninfected females. In this case, female individuals acquired Gfp-labelled Asaia, with highest infection rates 72-96 hours after mating and with increasing abundance of the tagged symbiont over time. When crosses between infected females and uninfected males were conducted, the occurrence of “female to male” transmission was observed, even though the transfer occurred unevenly.ConclusionsThe data presented demonstrate that the acetic acid bacterial symbiont Asaia is horizontally transmitted among S. titanus individuals both by co-feeding and venereal transmission, providing one of the few direct demonstrations of such a symbiotic transfer in Hemiptera. This study contributes to the understanding of the bacterial ecology in the insect host, and indicates that Asaia evolved multiple pathways for the colonization of S. titanus body.

show abstract

Section: Introductionmentioning

confidence: 85%

Horizontal transmission of the symbiotic bacterium Asaia sp. in the leafhopper Scaphoideus titanus Ball (Hemiptera: Cicadellidae)

et al. 2012

Self Cite

View full text Add to dashboard Cite

show abstract

“…The gut microbiota is of particular interest because it represents the first site of most extensive exposure to pathogens. Insect gut microbiomes, especially those of mosquitoes, have been characterized by either classical cultivation methods or by metagenomic analyses based on 16S rRNA sequencing [52–57]. The bacterial composition of mosquitoes sampled from natural habitats is highly variable but often contain a core microbiome that is dominated by a small number of taxa that can, however, vary depending on the insect species, geographical origin, ecological niche, and source of food, as well as sex [52–54,56].…”

Section: Mosquito Microbiota Influences Vector Competencementioning

confidence: 99%

The mosquito microbiota influences vector competence for human pathogens

Dennison

Jupatanakul

Dimopoulos

2014

Current Opinion in Insect Science

188

160

View full text Add to dashboard Cite

The midgut of insect vectors of human disease contains not only pathogens harmful to human health, but also a diverse microbiota. This microbiota can influence insects’ susceptibility to human pathogens, and the capacity to transmit them, through different mechanisms. Understanding the interaction between the vector, its microbiota and transmitted pathogens will provide novel opportunities to limit disease transmission.

show abstract

“…A number of bacterial species isolated from the mosquito midguts have also been utilized for manipulating their midgut microbiota (paratransgenesis), to modulate the vector competency of the mosquitoes, as a potent strategy for vector management [13–15]. Interestingly, the midgut microbial flora is dynamic and the diversity changes with the development of the vectors, in addition to other factors such as species, sex, life-stage, feeding behavior and geographical origin [16–22]. As a conventional assumption, midgut bacterial population is thought to be acquired from the environment, in which the said species develops.…”

Section: Introductionmentioning

confidence: 99%

Diversity of Cultivable Midgut Microbiota at Different Stages of the Asian Tiger Mosquito, Aedes albopictus from Tezpur, India

et al. 2016

View full text Add to dashboard Cite

Aedes aegypti and Ae. albopictus are among the most important vectors of arboviral diseases, worldwide. Recent studies indicate that diverse midgut microbiota of mosquitoes significantly affect development, digestion, metabolism, and immunity of their hosts. Midgut microbiota has also been suggested to modulate the competency of mosquitoes to transmit arboviruses, malaria parasites etc. Interestingly, the midgut microbial flora is dynamic and the diversity changes with the development of vectors, in addition to other factors such as species, sex, life-stage, feeding behavior and geographical origin. The aim of the present study was to investigate the midgut bacterial diversity among larva, adult male, sugar fed female and blood fed female Ae. albopictus collected from Tezpur, Northeastern India. Based on colony morphological characteristics, we selected 113 cultivable bacterial isolates for 16S rRNA gene sequence based molecular identification. Of the 113 isolates, we could identify 35 bacterial species belonging to 18 distinct genera under four major phyla, namely Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes. Phyla Proteobacteria and Firmicutes accounted for majority (80%) of the species, while phylum Actinobacteria constituted 17% of the species. Bacteroidetes was the least represented phylum, characterized by a single species- Chryseobacterium rhizoplanae, isolated from blood fed individuals. Dissection of midgut microbiota diversity at different developmental stages of Ae. albopictus will be helpful in better understanding mosquito-borne diseases, and for designing effective strategies to manage mosquito-borne diseases.

show abstract

Molecular Evidence for Multiple Infections as Revealed by Typing of Asaia Bacterial Symbionts of Four Mosquito Species

Cited by 83 publications

References 34 publications

Horizontal transmission of the symbiotic bacterium Asaia sp. in the leafhopper Scaphoideus titanus Ball (Hemiptera: Cicadellidae)

Horizontal transmission of the symbiotic bacterium Asaia sp. in the leafhopper Scaphoideus titanus Ball (Hemiptera: Cicadellidae)

The mosquito microbiota influences vector competence for human pathogens

Diversity of Cultivable Midgut Microbiota at Different Stages of the Asian Tiger Mosquito, Aedes albopictus from Tezpur, India

Contact Info

Product

Resources

About