Molecular Evidence for Dissemination of Unique
            <i>Campylobacter jejuni</i>
            Clones in Curaçao, Netherlands Antilles

Duim, Birgitta; Godschalk, Peggy C. R.; Braak, Nicole van den; Dingle, Kate E.; Dijkstra, Jeroen R.; Leyde, Ewald; Plas, J. van der; Colles, Frances M.; Endtz, Hubert P.; Wagenaar, Jaap A.; Maiden, Martin C. J.; Belkum, Alex van

doi:10.1128/jcm.41.12.5593-5597.2003

Cited by 61 publications

(72 citation statements)

References 22 publications

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“…However, MLST has been found to be as discriminatory as PFGE for distinguishing between temporally related isolates and the epidemic-causing isolates in different outbreaks caused by C. jejuni (42). The ability of MLST to have the same value for epidemiological typing as that of PFGE, AFLP, and ribotyping has also been demonstrated before (10). In the current study, PFGE resulted in good discrimination, but it did not discriminate as well as MLST.…”

Section: Discussionmentioning

confidence: 55%

Campylobacter coliin Swine Production: Antimicrobial Resistance Mechanisms and Molecular Epidemiology

Thakur

Gebreyes

2005

J Clin Microbiol

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The aim of this study was to determine antimicrobial resistance, to evaluate and compare the use of two genotyping methods for molecular epidemiology purposes, and to determine the genotypic diversity of Campylobacter coli of porcine origin. A total of 100 C. coli isolates from swine were tested for susceptibility to six antimicrobials using the agar dilution method and genotyped using two high-resolution fingerprinting approaches: multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). Evaluation of the methods was based on their resistance patterns, discriminatory indexes (DI), high test throughputs, costs, and turnaround times. Resistance to erythromycin and tetracycline was the most common. Both genotypic methods were found to have high discriminatory power, although MLST had a higher DI (0.936) than PFGE (DI ‫؍‬ 0.889). It also had a higher throughput than PFGE. Isolates were clustered into 27 groups by MLST compared to 11 by PFGE. MLST was able to further discriminate the isolates grouped under the same cluster by PFGE. Out of the 65 MLST sequence types (STs) identified among the total isolates, 50 were reported for the first time. Most STs were found to be specific to the farm (n ‫؍‬ 38) and to slaughter (n ‫؍‬ 22). Resistance against tetracycline and erythromycin was encoded by the tet(O) gene and a A2075G point mutation in the 23S rRNA gene, respectively. A high ciprofloxacin MIC (>64 g/liter) was conferred by a point mutation in the gyrA gene. The weak clonal structure of the C. coli population among swine was further highlighted by the index of association value of 0.293. The findings of this study indicate that multidrug-resistant diverse C. coli strains exhibiting resistance to ciprofloxacin and erythromycin are concerning, since these are the drugs of choice for treating invasive campylobacteriosis cases in humans.

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Section: Discussionmentioning

confidence: 55%

Campylobacter coliin Swine Production: Antimicrobial Resistance Mechanisms and Molecular Epidemiology

Thakur

Gebreyes

2005

J Clin Microbiol

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“…Although this method has been used successfully to characterize C. jejuni strains and identify clonal lineages within the species (6,11,13,43,60), it cannot characterize strains from other, clinically relevant Campylobacter species (e.g., C. coli and C. upsaliensis), nor can it address interspecies genetic exchange within the genus. Therefore, an expanded MLST typing system was developed, encompassing four additional species: C. coli, C. lari, C. upsaliensis, and C. helveticus.…”

Section: Discussionmentioning

confidence: 99%

“…The necessity of a reliable typing method to characterize C. jejuni strains and investigate the epidemiology of C. jejuni infections provided the impetus for the development of a multilocus sequence typing (MLST) system for C. jejuni. This method was developed by Dingle et al (12) and has been used successfully to characterize C. jejuni strains (11,13,43,59,63) and investigate C. jejuni outbreaks (60). As with MLST methods developed in other taxa, this system amplifies and sequences portions of seven housekeeping genes.…”

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confidence: 99%

Extended Multilocus Sequence Typing System for Campylobacter coli , C. lari , C. upsaliensis , and C. helveticus

On²,

et al. 2005

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A multilocus sequence typing (MLST) system has been reported previously for Campylobacter jejuni to both differentiate strains and identify clonal lineages. However, sequence variation at the MLST loci prevents its use for closely related Campylobacter species. We describe herein an expanded MLST method to include three clinically relevant Campylobacter species, C. coli, C. lari, and C. upsaliensis, and a fourth Campylobacter species, C. helveticus. The C. coli and C. helveticus methods use the same seven C. jejuni loci (aspA, atpA, glnA, gltA, glyA, pgm, and tkt); however, adk and pgi were substituted for aspA and gltA in C. lari and for gltA and pgm in C. upsaliensis. Multiple C. coli (n ‫؍‬ 57), C. lari (n ‫؍‬ 20), C. upsaliensis (n ‫؍‬ 78), and C. helveticus (n ‫؍‬ 9) isolates, representing both clinical and environmental sources, were typed. All four species were genetically diverse: the majority (>80%) of the isolates had unique sequence types (STs). Using this method, mixed C. lari, C. upsaliensis, and C. helveticus isolates were identified; upon separation, each isolate was shown to contain two strains of the same species with distinct STs. Additionally, the expanded MLST method was able to detect potential lateral transfer events between C. jejuni and either C. coli or C. lari and between C. upsaliensis and C. helveticus. Thus, the expanded MLST method will prove useful in differentiating strains of five Campylobacter species, identifying mixed Campylobacter cultures, and detecting genetic exchange within the genus.

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“…ST-21, ST-475, ST-918, and ST-937 were represented by both human and chicken isolates. Thirteen (43%) isolates belonged to novel STs, suggesting that unique STs circulate in local geographic regions (5).…”

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confidence: 99%

Genetic Diversity of Campylobacter sp. Isolates from Retail Chicken Products and Humans with Gastroenteritis in Central Michigan

Fitch¹,

Sachen²,

Wilder³

et al. 2005

J Clin Microbiol

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Multilocus sequencing was used to compare Campylobacter sp. strains isolated from retail chicken products and humans with gastroenteritis in central Michigan. Sequence comparisons demonstrated overlapping diversity between chicken and human isolates. Campylobacter jejuni isolates from clinical sources had a greater diversity of flagellin alleles and a higher rate of quinolone resistance than isolates from retail chicken products.

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Molecular Evidence for Dissemination of Unique Campylobacter jejuni Clones in Curaçao, Netherlands Antilles

Abstract: Campylobacter jejuni isolates (n ‫؍‬234

Cited by 61 publications

References 22 publications

Campylobacter coliin Swine Production: Antimicrobial Resistance Mechanisms and Molecular Epidemiology

Campylobacter coliin Swine Production: Antimicrobial Resistance Mechanisms and Molecular Epidemiology

Extended Multilocus Sequence Typing System for Campylobacter coli , C. lari , C. upsaliensis , and C. helveticus

Genetic Diversity of Campylobacter sp. Isolates from Retail Chicken Products and Humans with Gastroenteritis in Central Michigan

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