Molecular evidence for an ancient duplication of the entire yeast genome

Wolfe, Kenneth H.; Shields, Denis C.

doi:10.1038/42711

Cited by 1,639 publications

(1,367 citation statements)

References 19 publications

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“…genes exhibiting a significant BLASTP hit) are among the ca. 5800 genes of S. cerevisiae (Wolfe and Shields, 1997). It is discussed that the frequent presence of gene pairs reflects an ancient duplication of the whole genome.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of Saccharomyces cerevisiae Sco2p reveals a high degree of redundancy with Sco1p

Lode¹,

Paret²,

Rödel³

2002

Yeast

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The Saccharomyces cerevisiae gene SCO1 has been shown to play an essential role in the transfer of copper to the Cu A -centre of the mitochondrial cytochrome c oxidase subunit Cox2p. By contrast, the function of Sco2p, the gene product of the highly homologous SCO2 gene, remains to be elucidated. Deletion of the SCO2 gene does not affect growth on a variety of carbon sources, including glycerol, lactate and ethanol. We report here, that Sco2p is anchored in the mitochondrial membrane by a single transmembrane segment and displays a similar tripartite structure as Sco1p. Most parts of Sco1p can be replaced by the homologous parts of Sco2p without loss of function. A short stretch of 13 amino acids, immediately adjacent to the transmembrane region, is crucial for Sco1p function and cannot be replaced by its Sco2p counterpart. We propose that this region is relevant for the correct spatial orientation of the C-terminal part of the protein. Immunoprecipitation and in vitro binding assays show that Sco2p interacts with the C-terminal portion of Cox2p. This interaction is neither dependent on bound copper ions nor on the presence of Sco1p. Furthermore we report on in vitro binding assays which show that Sco2p can form homomeric complexes, but also heteromeric complexes with Sco1p. Our data suggest that Sco2p is involved in the transfer of copper to Cox2p, but that this activity is insufficient for oxidative growth and not able to substitute for Sco1p activity.

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Section: Discussionmentioning

confidence: 99%

Molecular characterization of Saccharomyces cerevisiae Sco2p reveals a high degree of redundancy with Sco1p

Lode¹,

Paret²,

Rödel³

2002

Yeast

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“…400 paralogous gene pairs. In support of the whole genome duplication hypothesis, it has been pointed out that several such pairs of Saccharomyces genes correspond to unique genes in K. lactis [11].…”

Section: What About the Whole Genome Duplication Hypothesis?mentioning

confidence: 93%

“…S. cerevisiae gene families containing six genes or more were grouped and considered as one large family whose mean size is 10 members. Pairs of S. cerevisiae paralogues (designed here as ancestral block pairs) are those contained in the ancestral blocks de¢ned in [11] and believed to have arisen by a whole genome duplication event occurred after the ancestors of S. kluyveri and S. cerevisiae became separated. The predicted 6213 ORFs for S. cerevisiae can be broken down into 887 genes of the ancestral block parts (1) and 5326 other genes (2).…”

Section: Expansion and Contraction Of Gene Familiesmentioning

confidence: 99%

Genomic Exploration of the Hemiascomycetous Yeasts: 20. Evolution of gene redundancy compared to Saccharomyces cerevisiae

et al. 2000

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We have evaluated the degree of gene redundancy in the nuclear genomes of 13 hemiascomycetous yeast species. Saccharomyces cerevisiae singletons and gene families appear generally conserved in these species as singletons and families of similar size, respectively. Variations of the number of homologues with respect to that expected affect from 7 to less than 24% of each genome. Since S. cerevisiae homologues represent the majority of the genes identified in the genomes studied, the overall degree of gene redundancy seems conserved across all species. This is best explained by a dynamic equilibrium resulting from numerous events of gene duplication and deletion rather than by a massive duplication event occurring in some lineages and not in others. ß 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

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“…The resulting increase in genome size and redundancy is counterbalanced by a high frequency of single gene deletion. With this hypothesis, there is no need to believe that S. cerevisiae would result from an ancestral whole-genome duplication as was proposed in [8].…”

Section: Introductionmentioning

confidence: 99%

Genomic Exploration of the Hemiascomycetous Yeasts: 18. Comparative analysis of chromosome maps and synteny withSaccharomyces cerevisiae

et al. 2000

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We have analyzed the evolution of chromosome maps of Hemiascomycetes by comparing gene order and orientation of the 13 yeast species partially sequenced in this program with the genome map of Saccharomyces cerevisiae. From the analysis of nearly 8000 situations in which two distinct genes having homologs in S. cerevisiae could be identified on the sequenced inserts of another yeast species, we have quantified the loss of synteny, the frequency of single gene deletion and the occurrence of gene inversion. Traces of ancestral duplications in the genome of S. cerevisiae could be identified from the comparison with the other species that do not entirely coincide with those identified from the comparison of S. cerevisiae with itself. From such duplications and from the correlation observed between gene inversion and loss of synteny, a model is proposed for the molecular evolution of Hemiascomycetes. This model, which can possibly be extended to other eukaryotes, is based on the reiteration of events of duplication of chromosome segments, creating transient merodiploids that are subsequently resolved by single gene deletion events. ß

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Molecular evidence for an ancient duplication of the entire yeast genome

Cited by 1,639 publications

References 19 publications

Molecular characterization of Saccharomyces cerevisiae Sco2p reveals a high degree of redundancy with Sco1p

Molecular characterization of Saccharomyces cerevisiae Sco2p reveals a high degree of redundancy with Sco1p

Genomic Exploration of the Hemiascomycetous Yeasts: 20. Evolution of gene redundancy compared to Saccharomyces cerevisiae

Genomic Exploration of the Hemiascomycetous Yeasts: 18. Comparative analysis of chromosome maps and synteny withSaccharomyces cerevisiae

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