Molecular Evidence for a Natural Primary Triple Hybrid in Plants Revealed from Direct Sequencing

Kaplan, Zdeněk; Fehrer, Judith

doi:10.1093/aob/mcm072

Cited by 55 publications

(63 citation statements)

References 67 publications

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“…In general, molecular approaches have been more effective than morphological or cytogenetic ones in the identification of interspecific hybridization (Kaplan and Fehrer, 2007).…”

Section: However Phylogenetic Relationships Among Species Ofmentioning

confidence: 99%

Phylogenetic Relationships in Korean Elaeagnus L. Based on nrDNA ITS Sequences

Son¹,

Yoon²,

Park³

2014

Korean Journal of Plant Resources

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-Molecular phylogenetic analyses of Korean Elaeagnus L. were conducted using seven species, one variety, one forma and four outgroups to evaluate their relationships and phylogeny. The sequences of internal transcribed spacer regions in nuclear ribosomal DNA were employed to construct phylogenetic relationships using maximum parsimony (MP) and Bayesian analysis. Molecular phylogenetic analysis revealed that Korean Elaeagnus was a polyphyly. E. umbellata var. coreana formed a subclade with E. umbellata. Additionally, the genetic difference between E. submacrophylla and E. macrophylla was very low. Moreover, E. submacrophylla formed a branch from E. macrophylla, indicating that E. submacrophylla can be regarded as a variety. However, several populations of this species were not clustered as a single clade; therefore, further study should be conducted using other molecular markers. Although E. glabra f. oxyphylla was distinct in morphological characters of leaf shape with E. glabra. But E. glabra f. oxyphylla was formed one clade by molecular phylogenetic with E. glabra. Additionally, this study clearly demonstrated that E. pungens occurs in Korea, although it was previously reported near South Korea in Japan and China. According to the results of ITS regions analyses, it showed a resolution and to verify the relationship between interspecies of Korean Elaeagnus.

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“…In general, molecular approaches have been more effective than morphological or cytogenetic ones in the identification of interspecific hybridization (Kaplan and Fehrer, 2007).…”

Section: However Phylogenetic Relationships Among Species Ofmentioning

confidence: 99%

Phylogenetic Relationships in Korean Elaeagnus L. Based on nrDNA ITS Sequences

Son¹,

Yoon²,

Park³

2014

Korean Journal of Plant Resources

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“…Our previous studies have revealed a very low level of intra-individual polymorphism in the multicopy nuclear ribosomal ITS region for Potamogeton species as well as relatively little intraspecific variation while hybrids, the majority of which are sterile, maintained the ribotypes of their respective parents (Kaplan & Fehrer 2007). These features provide an opportunity for the use of ITS sequencing as a very helpful tool in the detection of hybrids, which show additive patterns of their parents, i.e., superimposed peaks and insertions/deletions (indels) at diagnostic positions (Kaplan & Fehrer 2007).…”

Section: Identification Of Hybridsmentioning

confidence: 96%

“…Although character expression in hybrids is generally unpredictable (Rieseberg & Ellstrand 1993), several previous molecular studies (Hollingsworth et al 1995;Kaplan et al 2002;Fant & Preston 2004;Kaplan & Fehrer 2004Kaplan & Wolff 2004;Kaplan 2007) have demonstrated that for most Potamogeton and Stuckenia hybrids, either the parental species themselves or at least their respective species group can be reliably identified morphologically, as long as adequate expert inspection of a large set of key features is adopted. However, molecular proof of identification of Potamogeton hybrids is always advisable (Kaplan & Fehrer 2007), particularly if a previously unknown hybrid combination is suspected.…”

Section: Identification Of Hybridsmentioning

confidence: 99%

Potamogeton ×jacobsii (Potamogetonaceae) from New South Wales, Australia – the first Potamogeton hybrid from the Southern Hemisphere

Kaplan¹,

Fehrer²,

Hellquist

2011

Telopea

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Although more than 80 hybrids are documented for Potamogeton in the Northern Hemisphere, the hybrid diversity in the Southern Hemisphere is largely unknown. A taxonomically uncertain Potamogeton plant discovered in New South Wales, Australia, was subjected to detailed morphological investigation and molecular analyses to discover its exact identity. Both approaches resulted in the identification of the Potamogeton specimen as P. crispus × P. ochreatus, which is a previously unrecorded hybrid and is here described as Potamogeton × jacobsii nothosp. nova. Although P. crispus is known to hybridise with many other species, P. ×jacobsii is the first known hybrid involving P. ochreatus.

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“…Furthermore, in such a genus, hybridization is considered to be relatively frequent, and complex series of polyploidy and aneuploidy are present (e.g., Haynes 1974;Haynes and Hellquist 1978;Les 1983;Wiegleb 1988;Hollingsworth et al 1998;Kaplan et al 2002;Fant et al 2003). Both hybridization and polyploidy play significant roles in the evolution and diversification of various members of Potamogeton (Hollingsworth et al 1995;Kaplan and Fehrer 2007). In addition to phenotype plasticity, within Potamogeton, the chromosome numbers exhibit extensive variation both inter-and intraspecifically.…”

Section: Introductionmentioning

confidence: 99%

“…Since Wiegleb's (1988) comprehensive description of Potamogeton, increasing numbers of natural interspecific hybrids have been reported and identified that occurred between species either in the same or different ploidy levels (Kaplan et al 2002;Kaplan and Fehrer 2007;Lindqvist et al 2006;Du et al 2009;Wang et al 2007). P. 9 intortusifolius (He 1988), the synonym of P. anguillanus (Miki 1937;Lida and Kandono 2002), is a putative natural hybrid recorded in China.…”

Section: Introductionmentioning

confidence: 99%

A dynamic evolution of chromosome in subgenus Potamogeton revealed by physical mapping of rDNA loci detection

et al. 2012

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In this study, we analyzed 12 species in the subgenus Potamogeton (8 tetraploids and 2 diploids, 2 putative tetraploid hybrids) at the chromosomal level, including counting the chromosome number and physically mapping the rDNA. The extent of variation in the chromosome number and rDNA loci was determined in the Potamogeton species on both the inter-and intra-specific level. Moreover, one of the hybrid sets (P. perfoliatus # 9 P. wrightii $) was picked for performing artificial pollination for producing F1 generation adults and for sequential morphological character analysis and FISH detection. After comparing the parental species and natural hybrids from three different geographic locations, the intraspecific variations of rDNA loci were revealed in sampled plants; in addition, comparisons between artificial F1 and natural hybrids (P. 9 intortusifolius) showed a rapid change of 45S rDNA loci in response to the interspecific hybridization. We also compared our results concerning rDNA patterns with phylogenetic documents to derive complementary clues to karoytype evolution and interspecific relationships. Based on frequent hybridizations and active clonal reproduction with weak genetic selection, the rDNA chromosomal repatterning, such as the gain or loss of rDNA loci together with rDNA movements, might be one trend of chromosome evolution in this genus.

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Molecular Evidence for a Natural Primary Triple Hybrid in Plants Revealed from Direct Sequencing

Cited by 55 publications

References 67 publications

Phylogenetic Relationships in Korean Elaeagnus L. Based on nrDNA ITS Sequences

Phylogenetic Relationships in Korean Elaeagnus L. Based on nrDNA ITS Sequences

Potamogeton ×jacobsii (Potamogetonaceae) from New South Wales, Australia – the first Potamogeton hybrid from the Southern Hemisphere

A dynamic evolution of chromosome in subgenus Potamogeton revealed by physical mapping of rDNA loci detection

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