Molecular estimation of alteration in intestinal microbial composition in Hashimoto’s thyroiditis patients

Ishaq, Hafiz Muhammad; Mohammad, Imran Shair; Guo, Hui; Shahzad, Muhammad; Hou, Yin Jian; Ma, Chaofeng; Naseem, Zahid; Wu, Xiaokang; Shi, Peijie; Xu, Jiru

doi:10.1016/j.biopha.2017.08.101

Cited by 82 publications

(78 citation statements)

References 57 publications

(56 reference statements)

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“…The genus Parasutterella (Proteobacteria) has been defined as a core component of the healthy human and mouse gut microbiota and has been correlated with various health outcomes (33), including inflammatory bowel disease (IBD) (34), obesity (35,36), diabetes (37), fatty liver disease (38), chronic kidney disease (39), major depression (40), Henoch-Schönlein purpura in children (41), cholestasis in infants (42), and Hashimoto's thyroiditis in patients (43). A reduction of Parasutterella in response to an HFD has been observed in both animal models and human studies, indicating a positive correlation between Parasutterella abundance and HFD-induced metabolic phenotypes (44)(45)(46).…”

Section: Discussionmentioning

confidence: 99%

Correlations between α-Linolenic Acid-Improved Multitissue Homeostasis and Gut Microbiota in Mice Fed a High-Fat Diet

et al. 2020

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Previous studies have shown that α-linolenic acid (ALA) has a significant regulatory effect on related disorders induced by high-fat diets (HFDs), but little is known regarding the correlation between the gut microbiota and disease-related multitissue homeostasis. We systematically investigated the effects of ALA on the body composition, glucose homeostasis, hyperlipidemia, metabolic endotoxemia and systemic inflammation, white adipose tissue (WAT) homeostasis, liver homeostasis, intestinal homeostasis, and gut microbiota of mice fed an HFD (HFD mice). We found that ALA improved HFD-induced multitissue metabolic disorders and gut microbiota disorders to various degrees. Importantly, we established a complex but clear network between the gut microbiota and host parameters. Several specific differential bacteria were significantly associated with improved host parameters. Rikenellaceae_RC9_gut_group and Parasutterella were positively correlated with HFD-induced “harmful indicators” and negatively correlated with “beneficial indicators.” Intriguingly, Bilophila showed a strong negative correlation with HFD-induced multitissue metabolic disorders and a significant positive correlation with most beneficial indicators, which is different from its previous characterization as a “potentially harmful genus.” Turicibacter might be the key beneficial bacterium for ALA-improved metabolic endotoxemia, while Blautia might play an important role in ALA-improved gut barrier integrity and anti-inflammatory effects. The results suggested that the gut microbiota, especially some specific bacteria, played an important role in the process of ALA-improved multitissue homeostasis in HFD mice, and different bacteria might have different divisions of regulation. IMPORTANCE Insufficient intake of n-3 polyunsaturated fatty acids is an important issue in modern Western-style diets. A large amount of evidence now suggests that a balanced intestinal microecology is considered an important part of health. Our results show that α-linolenic acid administration significantly improved the host metabolic phenotype and gut microbiota of mice fed a high-fat diet, and there was a correlation between the improved gut microbiota and metabolic phenotype. Some specific bacteria may play a unique regulatory role. Here, we have established correlation networks between gut microbiota and multitissue homeostasis, which may provide a new basis for further elucidating the relationship between the gut microbiota and host metabolism.

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Section: Discussionmentioning

confidence: 99%

Correlations between α-Linolenic Acid-Improved Multitissue Homeostasis and Gut Microbiota in Mice Fed a High-Fat Diet

et al. 2020

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“…High-throughput was performed on twenty randomly selected stool samples in Hiseq 2500 platform (10 samples from healthy control and 10 samples from GD). In a previous study of gut microbial characterization, the same sample size was used by Ishaq et al 25 . As mentioned before the 16S rRNA gene with V3-V4 regions was amplified with primer: 341F (CCTAYGGGRBGCASCAG) 806R (GGACTACNNGGGTATCTAAT) TruSeq® DNA PCR-Free Sample Preparation Kit was used to construct the amplicons libraries.…”

Section: Methodsmentioning

confidence: 99%

Molecular Alteration Analysis of Human Gut Microbial Composition in Graves' disease Patients

Ishaq¹,

Mohammad²,

Shahzad³

et al. 2018

Int. J. Biol. Sci.

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The gut microbial association with host co-existence is critical for body homeostasis and pathogenicity. Graves' disease (GD) is an autoimmune disease manifested with hyperthyroidism and ophthalmopathy. However, we hypothesized that gut bacteria could affect an important role in GD pathogenicity. The current study aim was to characterize and investigate the intestinal bacterial composition of GD qualitatively and quantitatively. 27 GD and 11 healthy controls were enrolled for fecal sample collection. The PCR-DGGE of 16S rRNA gene by targeting V3 region and Real-time PCR for Lactobacillus, Bifidobacterium, Bacteroides vulgatus and Clostridium leptum, were performed. High-throughput sequencing of 16S rRNA gene with the V3+V4 site was perormed on Hiseq2500 platform on randomly 20 selected samples. The relative analysis of richness indices and diversity illustrated lesser diversification of intestinal bacteria in GD patients in contrast to controls. The data statistics shows the alteration in phyla of GD as compared to control. At the family taxonomic level, the relative abundance of Prevotellaceae and Pasteurellaceae were significantly higher in patients, while Enterobacteriaceae, Veillonellaceae, and Rikenellaceae were significantly lower in the diseased group as compared to control. At the genus level, a significant raised in genera count of the diseased group were Prevotella_9 and Haemophilus, while significantly decreased in the genera of the GD group were Alistipes and Faecalibacterium. The modulation in intestinal bacterial composition was checked at species level particularly H. parainfluenza abundance was raised in GD. The outcomes of the current study are aligned with the proposed hypothesis of gut microbial dysbiosis in GD. Statistically, alpha indices and differential abundance analyses of each intestinal bacterial community were significantly changed in GD. Therefore, the current study may provide a new insight into the GD pathogenesis and, in turn, explore its contribution in possible treatments.

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“…An alteration in the composition of intestinal bacteria (dysbiosis), a bacterial overgrowth increasing intestinal permeability, and a shift to proinflammatory cells are some of the factors of microbial impact on the thyroid [4,19]. Zhao et al and Ishaq et al investigated the microbial composition in euthyroid and hypothyroid HT patients and found a dysbiosis as well as a bacterial overgrowth in the hypothyroid patient group [22,23]. Lauritano et al assigned the bacterial overgrowth in connection with hypothyroidism mainly to the small intestine [24].…”

Section: Intestinal Influences On the Thyroidmentioning

confidence: 99%

Thyroid-Gut-Axis: How Does the Microbiota Influence Thyroid Function?

et al. 2020

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A healthy gut microbiota not only has beneficial effects on the activity of the immune system, but also on thyroid function. Thyroid and intestinal diseases prevalently coexist—Hashimoto’s thyroiditis (HT) and Graves’ disease (GD) are the most common autoimmune thyroid diseases (AITD) and often co-occur with Celiac Disease (CD) and Non-celiac wheat sensitivity (NCWS). This can be explained by the damaged intestinal barrier and the following increase of intestinal permeability, allowing antigens to pass more easily and activate the immune system or cross-react with extraintestinal tissues, respectively. Dysbiosis has not only been found in AITDs, but has also been reported in thyroid carcinoma, in which an increased number of carcinogenic and inflammatory bacterial strains were observed. Additionally, the composition of the gut microbiota has an influence on the availability of essential micronutrients for the thyroid gland. Iodine, iron, and copper are crucial for thyroid hormone synthesis, selenium and zinc are needed for converting T4 to T3, and vitamin D assists in regulating the immune response. Those micronutrients are often found to be deficient in AITDs, resulting in malfunctioning of the thyroid. Bariatric surgery can lead to an inadequate absorption of these nutrients and further implicates changes in thyroid stimulating hormone (TSH) and T3 levels. Supplementation of probiotics showed beneficial effects on thyroid hormones and thyroid function in general. A literature research was performed to examine the interplay between gut microbiota and thyroid disorders that should be considered when treating patients suffering from thyroid diseases. Multifactorial therapeutic and preventive management strategies could be established and more specifically adjusted to patients, depending on their gut bacteria composition. Future well-powered human studies are warranted to evaluate the impact of alterations in gut microbiota on thyroid function and diseases.

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Molecular estimation of alteration in intestinal microbial composition in Hashimoto’s thyroiditis patients

Cited by 82 publications

References 57 publications

Correlations between α-Linolenic Acid-Improved Multitissue Homeostasis and Gut Microbiota in Mice Fed a High-Fat Diet

Correlations between α-Linolenic Acid-Improved Multitissue Homeostasis and Gut Microbiota in Mice Fed a High-Fat Diet

Molecular Alteration Analysis of Human Gut Microbial Composition in Graves' disease Patients

Thyroid-Gut-Axis: How Does the Microbiota Influence Thyroid Function?

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