Molecular epidemiology of virulent E. coli among rural small scale dairy herds and shops: Efficacy of selected marine algal extracts and disinfectants

Ahmed, Ahmed; Diab, Hassan Mahmoud; Alkahtani, Mohammed Ali; Alshehri, Mohammed Ali; Saber, Hani; Badr, Heba; Dandrawy, Mohamed; El‐Mansi, Ahmed A.; Shati, Ali A.

doi:10.1080/09603123.2020.1727422

Cited by 13 publications

(17 citation statements)

References 49 publications

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“…The killing power of multiple treatments against bacteria was determined as log 10 reduction in colony-forming units (CFU). Moreover, the result is presented as log CFU/ml, a function of (IgY and/or probiotic) concentration in the test medium as shown before (20).…”

Section: Antimicrobial Bioactivity Assaymentioning

confidence: 99%

Egg Yolk IgY: A Novel Trend of Feed Additives to Limit Drugs and to Improve Poultry Meat Quality

Hussein

Rehan

et al. 2020

Front. Vet. Sci.

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Drugs that are commonly used in poultry farms can potentially cause a detrimental effect on meat consumers as a result of chemical residues. Therefore, seeking a natural alternative is crucial for the health of the consumers. The egg yolk immunoglobulin Y (IgY) is a promising natural replacement for antibiotics in the broilers' diet. There is a scarce focus on the influence of probiotics and IgY on the quality and the nutritive values of broiler meat and whether it can efficiently displace the anti-microbial power of antibiotics. Herein we used 40 Ross chicks (1.2 ± 0.43 days old) and separated them into four groups with variant feed additives (basal diet “control,” probiotic, IgY, and probiotic + IgY). Our findings showed that the combination of probiotic and IgY supplementation enhanced the carcass quality traits and decreased the pH values that could retard spoilage due to bacteria and improve shelf life and meat quality. The same group also achieved a significant reduction in thiobarbituric acid value, indicating an improvement of meat quality. Moreover, color, shear force, water holding capacity, and cooking loss were most acceptable in broiler meat supplemented with IgY, which confirmed the highest carcass quality. Notably, the weight gain in the combination group has been greatly increased. Also, the protein percentage was the highest (22.26 ± 0.29, P < 0.001) in this combined supplementation group, which revealed the highest nutritive values. Staphylococcus aureus and Escherichia coli could not be detected in the meat of the probiotics group and/or in the combined treatment group. Interestingly, the IgY group showed an evidence of the killing power (log colony-forming units per milliliter) of S. aureus and Listeria monocytogenes at 1,500 μg/ml. Our findings, in vitro as well as in vivo , revealed that the combination group had antimicrobial bioactivity and enhanced the chickens' immunity. Therefore, IgY, a novel trend of feed additives, can be used to limit drugs. Additionally, the mortality percentage recorded was zero in all groups that received feed supplementation, while the combination group reached the best financial advantages. We concluded that feeding IgY powder with probiotic is a frontier to improve the productivity, immunity, and meat quality of broilers.

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Section: Antimicrobial Bioactivity Assaymentioning

confidence: 99%

Egg Yolk IgY: A Novel Trend of Feed Additives to Limit Drugs and to Improve Poultry Meat Quality

Hussein

Rehan

et al. 2020

Front. Vet. Sci.

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“…The solvents petroleum ether, acetone, chloroform, methanol, ethanol, hexane, ethyl acetate, and water have been used for algal extraction to explore antibacterial activity against both Gram-positive and Gram-negative bacteria, including L. monocytogenes . 31 Globally, P. pavonica ethanolic extract exhibits the highest activity against L. monocytogenes growth, with an inhibition zone of 14 mm 53 and 13 mm against other Listeria spp. 54 In addition, methanolic ethanol, chloroform, acetone, ethyl acetate, and hexane extracts of P. pavonica show have shown activity against the growth of L. monocytogenes with 16±0.27, 14±0.11, 13±0.19, 11±0.22, 15±0.27, and 9±0.09 mm inhibition zones, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…The anti– L. monocytogenes activity of algae extracts and Ag-NPs– P. pavonica against virulent and QAC-resistant strains was detected using agar-well diffusion as per Zainol et al 34 and the liquid-broth method described by Ahmed et al 31 In the latter method, data are expressed as log CFU/mL as a function of alga-extract concentration in the assay medium. All assays were performed in triplicate, and the results are given as means of three independent experiments.…”

Section: Methodsmentioning

confidence: 99%

“…These species were identified according to standard taxonomic keys 29,30 Samples were collected in sterilized polyethylene bags and kept in an icebox for transport to the laboratory. Sample preparation and extraction were done as per to Ahmed et al 31 The weighted crude extracts were suspended in dimethyl sulfoxide (DMSO) to a final concentration of 50 mg/mL, then stored in airtight bottles in a refrigerator until used for experiments. Biosynthesis and Characterization of Silver Nanoparticles of P. pavonica (Ag-NPs-P. pavonica)…”

Section: Algal Collection and Extraction Preparationmentioning

confidence: 99%

“…Ag-NPs were synthesized by adding 10 mL of pure algal extracts to 90 mL of AgNO 3 (1 mM) in a 250 mL Erlenmeyer flask, heated in water bath at 60°C for 1 hour with continuous stirring, then incubated in the dark for 24 hours at room temperature. 31 Characterization of Ag-NPs-P. pavonica…”

Section: Algal Collection and Extraction Preparationmentioning

confidence: 99%

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Control of Virulent Listeria monocytogenes Originating from Dairy Products and Cattle Environment Using Marine Algal Extracts, Silver Nanoparticles Thereof, and Quaternary Disinfectants

El‐Zamkan

Hendy

Diab

et al. 2021

IDR

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Introduction: Listeria monocytogenes is an important foodborne pathogen of public-and animalhealth concern globally. The persistence of L. monocytogenes in the dairy-processing environment has multifactorial causes, including lack of hygiene, inefficient cleaning, and improper disinfection practices. Materials and Methods: A total of 300 dairy-product and environmental samples were collected from dairy-cattle facilities and local dairy shops and vendors in Qena, Egypt. Samples were screened for the incidence of Listeria spp. and to detect virulence determinants and disinfectant-resistance genes. Three marine algal species -Caulerpa racemosa, Jania rubens, and Padina pavonica -were collected from Hurghada on the Red Sea coast. Algal extracts were screened using gas chromatography-mass spectrometry. The antimicrobial activity of some marine algal extracts, nanoparticles derived therefrom, and some disinfectants against L. monocytogenes strains were assessed in vitro using agar-well diffusion and liquid-broth methods. The impact of P. pavonica extract on the growth and survival of virulent L. monocytogenes in cheese and whey were clarified. Results and Discussion: The incidence of L. monocytogenes in dairy products and environmental samples was 15.5% and 19%, respectively. The most common toxigenic gene profile found among the isolates was hlyA + -inlA + -prfA + . The sensitivity pattern of L. monocytogenes strains to disinfectant containing alkyl (C 12-16 ) dimethyl BAC was high compared to other tested quaternary ammonium compounds (QAC) disinfectants tested, which showed lower log reductions against resistant strains. The QAC disinfectant-resistance gene qacH was detected in 40% of the isolates. Potent bactericidal activity of a petroleum ether extract of P. pavonica and silver nanoparticles of P. pavonica were obtained against the virulent L. monocytogenes strain. The population of L. monocytogenes in cheese curd and whey after 14 days was reduced at a rate of 9 log CFU/g and 8 log CFU/mL, respectively due to the effect of P. pavonica extract. After 28 days of storage, L. monocytogenes was completely inactivated in those dairy products. Conclusion: P. pavonica extract showed promising antimicrobial properties, calling for further comprehensive studies prior to it being applied in the food industry to enhance the safety, quality, and shelf life of products and protect public health.

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UV-Induced Antibacterial Activity of Green-Synthesized TiO2 Nanoparticles for the Potential Reuse of Raw Surface and Underground Water

Salem

El-Deen²,

Ebnalwaleed

et al. 2021

J Plant Growth Regul

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Molecular epidemiology of virulent E. coli among rural small scale dairy herds and shops: Efficacy of selected marine algal extracts and disinfectants

Cited by 13 publications

References 49 publications

Egg Yolk IgY: A Novel Trend of Feed Additives to Limit Drugs and to Improve Poultry Meat Quality

Egg Yolk IgY: A Novel Trend of Feed Additives to Limit Drugs and to Improve Poultry Meat Quality

Control of Virulent Listeria monocytogenes Originating from Dairy Products and Cattle Environment Using Marine Algal Extracts, Silver Nanoparticles Thereof, and Quaternary Disinfectants

UV-Induced Antibacterial Activity of Green-Synthesized TiO2 Nanoparticles for the Potential Reuse of Raw Surface and Underground Water

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