Molecular epidemiology of vancomycin-resistant Enterococcus faecium strains isolated from haematological malignancy patients in a research hospital in Turkey

Kırdar, Sevin; Şener, Aslı Gamze; Arslan, Uğur; Yurtsever, Süreyya Gül

doi:10.1099/jmm.0.012625-0

Cited by 14 publications

(16 citation statements)

References 30 publications

(32 reference statements)

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“…Odmienne obserwacje uzyskano w wyniku dochodzenia epidemiologicznego w szpitalu akademickim w Turcji. (16). Po wyizolowaniu E. faecium VRE z krwi pięciu pacjentów oddziału hematologicznego przeprowadzono dochodzenie epidemiologiczne polegające na badaniu wymazów z odbytu pacjentów i personelu oraz ze środowiska.…”

Section: Discussionunclassified

Genetic typing of Enterococcus faecium VRE strains isolated in three hospitals in Warsaw and Siedlce in 2015-2016

et al. 2019

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INTRODUCTION. Since the first report of vancomycin-resistant enterococci (VRE) in Poland, in 1996, these strains have spread in Polish hospitals, mainly due to selective pressure associated with increased use of vancomycin in the treatment of infections caused by methicillin-resistant staphylococci and Clostridium difficile. At the beginning of 2016 a growing number of patients colonized with VRE in the gastrointestinal tract was observed in the Children’s Memorial Health Institute (IPCZD). Some of these patients were transferred from other hospitals, and VRE colonization was found on admission. AIM. To analyze genetic similarity of VRE strains isolated from patients hospitalized in IPCZD and two other hospitals in Mazovian district, genetic typing by pulsed field gel electrophoresis (PFGE) was performed. MATERIALS AND METHODS. VRE strains were isolated from rectal swabs, and other clinical samples such as blood, cerebrospinal fluid, other body fluids, and environmental samples. A total of 56 VRE strains from IPCZD, 20 strains from Siedlce and 4 strains from patients from Grochowski Hospital in Warsaw were typed by PFGE. RESULTS. PFGE typing revealed 4 VRE clones containing several strains with identical restriction patterns. Among VRE strains isolated from neonates hospitalized in IPCZD, two clones with 24 and 20 identical strains were found. Respectively, 16 (67%) and 12 (60%) isolates were originated from rectal swabs from patients at admission to the hospital. Clonal strains were identified in all three hospitals included in the study. CONCLUSIONS. Our results showed that VRE strains had spread in the region. Isolation of clonal strains on admission to the hospital suggested independent VRE introductions from environment or other hospitals. Identification of clonal strains obtained from rectal swabs and other clinical samples during hospitalization indicated horizontal transmission.

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Section: Discussionunclassified

Genetic typing of Enterococcus faecium VRE strains isolated in three hospitals in Warsaw and Siedlce in 2015-2016

et al. 2019

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“…In the study, MLST analysis of 8 VREfm isolates revealed three different STs: ST78, ST203 and ST17. ST17, ST78 and ST203 belonged to the clonal complex-17 (CC17) lineage, which is the cause of most of the nosocomial VRE outbreaks in Asia, Europe and Latin America, including in China [28][29][30][31]. The hospital-adapted CC17 has rapidly spread globally during the last two decades [4,28,32].…”

Section: Pcr Analysis Of Virulence Genesmentioning

confidence: 99%

Molecular Characterization and Resistant Spectrum of Enterococci Isolated from a Haematology Unit in China

Shi

Zhao

et al. 2015

JCDR

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“…Multi-drug resistance is commonly seen in people who have recently been treated with antibiotics (17 (18,20). Kirdar et al (14) showed that all 12 E. faecium were isolated from the hematology unit. They detected VanA gene by PCR yet VanB and VanC genes were not found in the 12 VRE strains (14).…”

Section: Discussionmentioning

confidence: 99%

Genotyping of Vancomycin Resistant Enterococci in Arak Hospitals

Zadeh

Shojapour

Nazari

et al. 2015

Jundishapur J Microbiol

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Background: Enterococcal species have emerged as important pathogens in Iran as well as throughout the world. With the increased use of vancomycin, Vancomycin-Resistant Enterococci (VRE) has become an important nosocomial pathogen. Objectives: The aim of the present study was to determine the incidence and antimicrobial susceptibility pattern of VRE and also to determine the most important genes that cause resistance to vancomycin in clinical samples in Arak, Iran. Materials and Methods:In total, 200 enterococci samples were collected from clinical specimens of Arak hospitals. Enterococcal species were identified using standard biochemical tests. Antibiotic susceptibility was tested by the Clinical and Laboratory Standards Institute (CLSI) disk diffusion. Minimum Inhibitory Concentration (MICs) was determined by broth micro dilution. All of the VRE isolates were examined by PCR to detect the presence of VRE genes. Results: Disk diffusion agar showed that 96 strains (48%) were resistant to gentamicin, 89 (44.5%) to ciprofloxacin, 127 (63.5%) to erythromycin, 142 (71%) to tetracycline, 11 (5.5%) to teicoplanin, 32 (16%) to vancomycin, none to linezolid and 96 (48%) to co-trimoxazole. The MICs of the resistant isolates were as follows; 88 strains had MIC ≥ 32 μg/mL to vancomycin and 59 strains had MIC ≥ 32 μg/mL to teicoplanin. Molecular studies revealed that 59.09% of VRE contained VanA genes and 7.95% of VRE contained the VanB genes. None of the strains had vanC1 and vanC2/3 gene. Conclusions: According to the results of this study, rates of vancomycin-resistance in enterococci, in Iran like other parts of the world, is increasing. Therefore accurate methods are required for identifying strains that possess resistance genes because many cases of hospital infections are caused by these strains.

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Molecular epidemiology of vancomycin-resistant Enterococcus faecium strains isolated from haematological malignancy patients in a research hospital in Turkey

Cited by 14 publications

References 30 publications

Genetic typing of Enterococcus faecium VRE strains isolated in three hospitals in Warsaw and Siedlce in 2015-2016

Genetic typing of Enterococcus faecium VRE strains isolated in three hospitals in Warsaw and Siedlce in 2015-2016

Molecular Characterization and Resistant Spectrum of Enterococci Isolated from a Haematology Unit in China

Genotyping of Vancomycin Resistant Enterococci in Arak Hospitals

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