Molecular epidemiology of the SH (small hydrophobic) gene of human respiratory syncytial virus (HRSV), over 2 consecutive years

Lima, Hildener Nogueira; Botosso, Viviane Fongaro; Oliveira, Danielle Bruna Leal; Campos, Angélica Cristine de Almeida; Leal, Andréa L.; Silva, Tereza Souza; Bosso, Patrícia Alves Ramos; Moraes, Cláudia Trigo Pedroso de; Filho, Claudionor Gomes da Silva; Vieira, Sandra Elisabete; Gilio, Alfredo Elias; Stewien, Klaus Eberhard; Durigon, Edison Luíz

doi:10.1016/j.virusres.2011.08.013

Cited by 8 publications

(8 citation statements)

References 35 publications

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“…Important epidemiological data are generated by analysis of antigenic and genomic characterization of circulating HRSV in a particular region. There is limited information regarding the molecular epidemiology of HRSV in Brazil, and only one published study has analyzed HRSV strains circulating in a city in the country's Northeast region . Limited financial resources led to the small number of HRSV strains analyzed by sequencing in the present study.…”

Section: Discussionmentioning

confidence: 97%

“…There is limited information regarding the molecular epidemiology of HRSV in Brazil, and only one published study has analyzed HRSV strains circulating in a city in the country's Northeast region. 13,[20][21][22][23][24][25][26] Limited financial resources led to the small number of HRSV strains analyzed by sequencing in the present study. Nevertheless, the results of this analysis represent the first data on molecular epidemiology of HRSV in a Brazilian equatorial region.…”

Section: Discussionmentioning

confidence: 99%

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Respiratory syncytial virus epidemic periods in an equatorial city of Brazil

Moura

Perdigão

Ribeiro

et al. 2013

Influenza Resp Viruses

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BackgroundCharacterization of the human respiratory syncytial virus (HRSV) season at the local level has important implications for appropriate decisions on the time period for administration of specific prophylaxis.Objectives(1) To describe five consecutive epidemic periods of HRSV in an equatorial city of Brazil and (2) to show preliminary data on genomic diversity of circulating HRSV.Patients/MethodsNasopharyngeal aspirates of 2885 children attending the emergency room and wards of a public hospital were collected and screened by indirect immunofluorescence for HRSV infections during five consecutive years (from January 2004 to December 2008). In addition, the genetic and antigenic variability of the HRSV strains isolated was evaluated by partial nucleotide sequencing of the protein G gene.Results HRSV was detected in 15·8% of the analyzed samples. HRSV seasons occurred in a restricted period of each year. The onset of each HRSV season was variable (February to May), but the end always occurred in July. From the 456 HRSV infections found, 86 cases with bronchiolitis were genotyped. Both HRSV subgroups (A and B) cocirculated during the five epidemic periods. The 58 HRSV‐A strains grouped into two clades, GA2 and GA5. In respect of the HRSV‐B strains, the 28 samples grouped into two clades: GB3 and BA.Conclusions HRSV accounts for a substantial proportion of ARI in the study population. As in temperate countries, HRSV infections in this equatorial area of Brazil also cause seasonal yearly epidemics, and this has implications for prophylaxis strategies. The city of Fortaleza follows the same worldwide trend of circulation of genotypes of HRSV.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Respiratory syncytial virus epidemic periods in an equatorial city of Brazil

Moura

Perdigão

Ribeiro

et al. 2013

Influenza Resp Viruses

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“…The SH, it is a highly conserved small surface protein [38], [39], with a hydrophobic core possibly corresponding to a single transmembrane domain [40]. Molecular modeling studies have suggested that pentamers or hexamers [41] are formed with a circular structure and a central pore [42].…”

Section: Introductionmentioning

confidence: 99%

Gene Sequence Variability of the Three Surface Proteins of Human Respiratory Syncytial Virus (HRSV) in Texas

et al. 2014

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Human respiratory syncytial virus (HRSV) has three surface glycoproteins: small hydrophobic (SH), attachment (G) and fusion (F), encoded by three consecutive genes (SH-G-F). A 270-nt fragment of the G gene is used to genotype HRSV isolates. This study genotyped and investigated the variability of the gene and amino acid sequences of the three surface proteins of HRSV strains collected from 1987 to 2005 from one center. Sixty original clinical isolates and 5 prototype strains were analyzed. Sequences containing SH, F and G genes were generated, and multiple alignments and phylogenetic trees were analyzed. Genetic variability by protein domains comparing virus genotypes was assessed. Complete sequences of the SH-G-F genes were obtained for all 65 samples: HRSV-A = 35; HRSV-B = 30. In group A strains, genotypes GA5 and GA2 were predominant. For HRSV-B strains, the genotype GB4 was predominant from 1992 to 1994 and only genotype BA viruses were detected in 2004–2005. Different genetic variability at nucleotide level was detected between the genes, with G gene being the most variable and the highest variability detected in the 270-nt G fragment that is frequently used to genotype the virus. High variability (>10%) was also detected in the signal peptide and transmembrane domains of the F gene of HRSV A strains. Variability among the HRSV strains resulting in non-synonymous changes was detected in hypervariable domains of G protein, the signal peptide of the F protein, a not previously defined domain in the F protein, and the antigenic site Ø in the pre-fusion F. Divergent trends were observed between HRSV -A and -B groups for some functional domains. A diverse population of HRSV -A and -B genotypes circulated in Houston during an 18 year period. We hypothesize that diverse sequence variation of the surface protein genes provide HRSV strains a survival advantage in a partially immune-protected community.

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“…Brazil is a country of large territorial extension, but few studies have evaluated HRSV circulation patterns and genotypes, which are limited to hospital-based studies performed at Southeast and Northeast regions [20,21,22,24,25,26,22,24]. Studies including children that attended day care centers have been done mainly in Scandinavia, the United States and England [27], and evidenced that children who attended day care centers from the beginning of infancy present higher risks of respiratory infections comparing to children that did not attend day care centers [28].…”

Section: Resultsmentioning

confidence: 99%

“…Few studies have evaluated the epidemiology and HRSV genotypes circulating in São Paulo State, Brazil [20,21,22,23,24,25,26] and furthermore this is the first report on HRSV genotypes isolated from children attending a day care center in Brazil. Accordingly, we aimed to identify HRSV strains features by analyzing the genetic variability in the second hypervariable region of the attachment (G) gene of viruses isolated from clinical samples collected in a public day care center, and a public hospital in São José do Rio Preto-SP (SJRP), Brazil.…”

Section: Introductionmentioning

confidence: 99%

Diversity and Adaptation of Human Respiratory Syncytial Virus Genotypes Circulating in Two Distinct Communities: Public Hospital and Day Care Center

Gardinassi

Simas

Gomes

et al. 2012

Viruses

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HRSV is one of the most important pathogens causing acute respiratory tract diseases as bronchiolitis and pneumonia among infants. HRSV was isolated from two distinct communities, a public day care center and a public hospital in São José do Rio Preto – SP, Brazil. We obtained partial sequences from G gene that were used on phylogenetic and selection pressure analysis. HRSV accounted for 29% of respiratory infections in hospitalized children and 7.7% in day care center children. On phylogenetic analysis of 60 HRSV strains, 48 (80%) clustered within or adjacent to the GA1 genotype; GA5, NA1, NA2, BA-IV and SAB1 were also observed. SJRP GA1 strains presented variations among deduced amino acids composition and lost the potential O-glycosilation site at amino acid position 295, nevertheless this resulted in an insertion of two potential O-glycosilation sites at positions 296 and 297. Furthermore, a potential O-glycosilation site insertion, at position 293, was only observed for hospital strains. Using SLAC and MEME methods, only amino acid 274 was identified to be under positive selection. This is the first report on HRSV circulation and genotypes classification derived from a day care center community in Brazil.

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Molecular epidemiology of the SH (small hydrophobic) gene of human respiratory syncytial virus (HRSV), over 2 consecutive years

Cited by 8 publications

References 35 publications

Respiratory syncytial virus epidemic periods in an equatorial city of Brazil

Respiratory syncytial virus epidemic periods in an equatorial city of Brazil

Gene Sequence Variability of the Three Surface Proteins of Human Respiratory Syncytial Virus (HRSV) in Texas

Diversity and Adaptation of Human Respiratory Syncytial Virus Genotypes Circulating in Two Distinct Communities: Public Hospital and Day Care Center

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