Molecular epidemiology of the endemic multiresistance plasmid pSI54/04 of Salmonella Infantis in broiler and human population in Hungary

Abstract

1Salmonella Infantis (SI) became endemic in Hungary where the PFGE cluster B, 2 characterized by a large multiresistance (MDR) plasmid emerged among broilers leading to 3 an increased occurrence in humans. We hypothesised that this plasmid (pSI54/04) assisted 4 dissemination of SI. Indeed, Nal-Sul-Tet phenotypes carrying pSI54/04 occurred increasingly 5 between 2011-2013 among SI isolates from broilers and humans. Characterization of 6 pSI54/04 based on genome sequence data of the MDR strain SI54/04 indicated a size of ~277 7 kb and a high sequence similarity with the megaplasmid pESI of SI predominant in Israel. 8Molecular characterization of 78 representative broiler and human isolates detected the 9 prototype plasmid pSI54/04 and its variants of together with novel plasmid associations 10 within the emerging cluster B. To test in vitro and in vivo pathogenicity of pSI54/04 we 11 produced plasmidic transconjugant of the plasmid-free pre-emergent strain SI69/94. This 12 parental strain and its transconjugant have been tested on chicken embryo fibroblasts (CEFs) 13 and in orally infected day old chicks. The uptake of pSI54/04 did not increase the 14 pathogenicity of the strain SI69/94 in these systems. Thus, dissemination of SI in poultry 15 could be assisted by antimicrobial resistance rather than by virulence modules of the endemic 16 plasmid pSI54/04 in Hungary. 17

Abstracts of the 5th Central European Forum for Microbiology, October 18-20, 2017, Keszthely, Hungary

“…We found 27 dsRNS-harbouring strains from the total 221 investigated isolates. We found dsRNA elements in 23% of the investigated Umbelopsis; in these isolates four different patterns were observed with 1 to 4 discrete and different sized (2,3 Soil biota plays an important role in transformation of the compounds of toxic metals. The biomass of soil fungi rating 35-40% of total biomass can adsorb the majority of these substances, at the same time it forms a significant source of food chains there.…”

“…ANDREA CSONKA 1,2 3,4,5 , GABRIELLA SPENGLER Multidrug resistance (MDR) is a serious problem for the treatment of bacterial infections due to the appearance of reduced or missing response of microorganisms to the applied antimicrobial agents. The emergence of MDR bacteria is considered a serious public health threat, for this reason the design of new antibacterial agents as adjuvants compounds of the antibiotic therapy is gaining more attention.…”

“…C. trachomatis D is of great public health significance because of the impacts of untreated diseases on reproductive outcomes. Selenocompounds (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11) were compared based on their antibacterial activity in AcrAB-TolC expressing Escherichia coli K-12 AG100 and AG100A, Staphylococcus aureus ATCC 25923 and Enterococcus faecalis ATCC 29212 strains. The minimum inhibitory concentrations (MICs) of selenocompounds were determined by two-fold broth microdilution method in 96-well plates.…”

“…Coagulation is another downstream operation, in which the industry use bacterial, fungal, chemical and algal coagulants aid in the flotation or settling of microalgae. [1][2][3] In this research we made microscale fermentations in a 24 well microplate. The volume of a well was 3 ml and we put inside 1 ml algae suspension.…”

“…This treatment will result in deproteinized whey permeate, which is then further processed by nanofiltration, when the lactose concentration is increased to over 200 g/dm 3 . While whey proteins represent high market value, finding ways for the utilization of the lactose syrup is still a challenging research task.…”