Molecular Epidemiology of Staphylococcus aureus among Patients with Skin and Soft Tissue Infections in Two Chinese Hospitals

Gu, Fei-Fei; Chen, Ye; Dong, De-Ping; Song, Zhen; Guo, Xia; Ni, Yizhao; Han, Lizhong

doi:10.4103/0366-6999.190673

Cited by 17 publications

(19 citation statements)

References 25 publications

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“…These results were confirmed in a multicentric study in 12 provinces, distributed in distant geographic areas with a prevalence of 7.8% in 2010 and 10.1% in 2016, among bacteremia isolates [60]. In patients with SSTI, the prevalence of MSSA CC398 increased from 11.6 (11/95) to 17.4% (8/46) between 2011 and 2015 [57,58]. The first isolate was reported during the period 1994-1998 [64].…”

Section: In Chinasupporting

confidence: 54%

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Human Infection of Methicillin-Susceptible Staphylococcus aureus CC398: A Review

et al. 2020

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Staphylococcus aureus (SA) belonging to the clonal complex 398 (CC398) took a special place within the species due to its spread throughout the world. SA CC398 is broadly separated in two subpopulations: livestock-associated methicillin-resistant SA (MRSA) and human-associated methicillin-susceptible SA (MSSA). Here, we reviewed the global epidemiology of SA CC398 in human clinical infections and focused on MSSA CC398. The last common ancestor of SA CC398 was probably a human-adapted prophage φSa3-positive MSSA CC398 strain, but the multiple transmissions between human and animal made its evolution complex. MSSA and MRSA CC398 had different geographical evolutions. Although MSSA was present in several countries all over the world, it was mainly reported in China and in France with a prevalence about 20%. MSSA CC398 was frequently implicated in severe infections such as bloodstream infections, endocarditis, and bone joint infections whereas MRSA CC398 was mainly reported in skin and soft tissue. The spread of the MSSA CC398 clone is worldwide but with a heterogeneous prevalence. The prophage φSa3 played a crucial role in the adaptation to the human niche and in the virulence of MSSA CC398. However, the biological features that allowed the recent spread of this lineage are still far from being fully understood.

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Section: In Chinasupporting

confidence: 54%

“…As observed in France, MSSA CC398 is the most prevalent SA lineage in China with a prevalence varying from 5.5 to 26.6% [21][22][23][53][54][55][56][57][58][59][60][61][62][63][64]. This lineage was equally retrieved in patients with SSTI or BSI.…”

Section: In Chinamentioning

confidence: 59%

Human Infection of Methicillin-Susceptible Staphylococcus aureus CC398: A Review

et al. 2020

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“…The livestock-associated clone CC398 was observed secondly frequent in this study. In our previous study, CC398 was the most common clone among patients with skin and soft tissue infections (SSTIs) especially where the livestock husbandry well developed in China 31 . In recent years in Denmark, the percentage of LA-MRSA CC398 among BSIs and SSTIs has been increasing and reached its peak in 2014, accounting for 16% and 21% in MRSA BSIs and SSTIs respectively 32 .…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance and Molecular Epidemiology of Staphylococcus aureus Causing Bloodstream Infections at Ruijin Hospital in Shanghai from 2013 to 2018

Xiao

et al. 2020

Sci Rep

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Staphylococcus aureus or methicillin-resistant Staphylococcus aureus (MRSA) is an important issue associated with significant morbidity and mortality and well known as a predominant pathogen causing bloodstream infection (BSis) globally. to estimate the antibiotic resistance and molecular characteristics of S. aureus causing BSIs in Shanghai, 120 S. aureus isolates (20 isolates each year) from the patients with S. aureus BSIs from 2013 to 2018 were randomly selected and enrolled in this study. Fifty-three (44.2%) MRSA isolates were determined, and no isolate was found resistant to vancomycin, daptomycin, synercid, linezolid and ceftaroline. The toxin genes tst, sec, seg and sei were found more frequently among MRSA isolates compared with MSSA isolates (all P < 0.0001). Twentynine sequence types (STs) were identified, and ST5 (23.3%) was the most common ST, followed by ST398 (11.7%) and ST764 (10.0%). SCCmec II (73.6%) was the most frequent SCCmec type among MRSA isolates. The dominant clonal complexes (CCs) were CC5 (ST5, ST764, ST965 and ST3066; 36.7%) and the livestock-associated clone CC398 (ST398, 11.7%). MRSA-CC5 was the predominant CC among MRSA isolates (37/53, 69.8%), and CC5-II MRSA was found in 34 isolates accounting for 91.9% (34/37) among CC5 MRSA isolates. In addition, all 29 tst-positive MRSA isolates were CC5-MRSA as well. Our study provided the properties and genotypes of S. aureus causing BSis at Ruijin Hospital in Shanghai from 2013 to 2018, and might suggest of value clues for the further study insights into pathogenic mechanisms intrinsically referring to the development of human-adapted S. aureus clones and their diffusions. Bloodstream infections (BSIs) is a severe infection with high incidence and lethality all over the world, and it always prolonged hospital stay for a long period 1. It has been reported to be one of the seven leading causes of death in North America and Europe 2. Staphylococcus aureus is well known as one of the most important human pathogens across the world and is capable of causing a variety of infections in healthcare facilities and communities. Furthermore, S. aureus is one of the major and most fatal causes of bacteremia with an estimated mortality of 20%, and at least 50% of patients with S. aureus bacteremia (SAB) will develop complicated bacteremia 3. S. aureus is one of the most common causes of severe BSIs with high morbidity and mortality. Early mortality associated with SAB appears to have plateaued at approximately 20-30% 4 , and imposes a substantial burden on patients and healthcare systems. In the United States, the annual incidence of SAB is 4.3 to 38.2 per 100,000 person-years, and the 30-day all-cause mortality of SAB is 20% and has not changed since the 1990s 5. In Ireland,

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“…The frequency of agr I was higher than previous reports in Chinese children [25,28]. To explain, clonal differences of strains should be regarded.…”

Section: Discussionmentioning

confidence: 59%

Association between agr group, genetic background, virulence factors and disease types of Staphylococcus aureus isolated from Chinese children

Yan¹,

Qian²,

Yao³

et al. 2020

Preprint

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Background Staphylococcus aureus (S. aureus) accessory gene regulator (agr) system plays a critical role in staphylococcal pathogenesis. Our study aimed to investigate the relationship between agr group, the genetic background, virulence genes and disease types distribution of S. aureus isolated from different clinical sources among Chinese children.Methods S. aureus strains were isolated from Beijing Children’s hospital from October 2017 to October 2019. Isolates were typed by multilocus sequence typing (MLST), staphylococcal protein A (spa), agr, and staphylococcal cassette chromosome mec (SCCmec) typing (for methicillin-resistant S. aureus [MRSA] isolates). Furthermore, all isolates were tested for the presence of 19 selected virulence genes.Results A total of 191 non-repetitive S. aureus clinical isolates were collected and the agr type I was the most prevalent (84.8%). S. aureus isolates were divided into 33 sequence types (STs), 20 clonal complexes (CCs) and 59 spa types, ST59 (39.8%) and t437 (37.7%) were the predominant types. CC59, CC25, CC22, CC951, CC8, and CC398 isolates possessed agr group I; CC15 isolates harbored agr group II; CC30 strains were characterized as agr group III, and CC121 harbored agr group IV. Of the 19 virulence genes, the tst gene was more prevalent among agr group III compared to other groups (p = 0.006); eta and etb genes were more prevalent among agr group IV than other groups (p = 0.003 and 0.001, respectively); nearly all strains that harbored the lukS/F-PV gene (98.3%) belonged to agr group I (p = 0.004); the frequencies of bbp and ebpS genes belonged to agr group I were statistically lower than that of other groups (p < 0.001). Among 161 diagnoses, the frequency of strains from cellulitis patient harbored agr group III was higher than that of other groups (p = 0.046), and one strain isolated from staphylococcal scalded skin syndrome (SSSS) patient, which was identified as agr type IV (p = 0.021).Conclusions The results indicated that the S. aureus agr type was linked to the genetic background. Besides, a possible relationship between the agr group, several virulence determinants, and specific disease types was observed.

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Molecular Epidemiology of Staphylococcus aureus among Patients with Skin and Soft Tissue Infections in Two Chinese Hospitals

Cited by 17 publications

References 25 publications

Human Infection of Methicillin-Susceptible Staphylococcus aureus CC398: A Review

Human Infection of Methicillin-Susceptible Staphylococcus aureus CC398: A Review

Antimicrobial Resistance and Molecular Epidemiology of Staphylococcus aureus Causing Bloodstream Infections at Ruijin Hospital in Shanghai from 2013 to 2018

Association between agr group, genetic background, virulence factors and disease types of Staphylococcus aureus isolated from Chinese children

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