Molecular epidemiology of respiratory syncytial virus for 28 consecutive seasons (1990-2018) and genetic variability of the duplication region in the G gene of genotypes ON1 and BA in South Korea

Yun, Ki Wook; Choi, Eun Hwa; Lee, Hoan Jong

doi:10.1007/s00705-020-04580-z

“…Specifically, with this lab testing data, a transition from RSV-B to RSV-A was observed between the 2016 and 2017 seasons in WA (data not shown). These results are consistent with other molecular epidemiological studies globally that show both RSV-A and RSV-B subtypes co-circulate with shifting predominance across seasons ( Di Giallonardo et al., 2018 ; Luo et al., 2020 ; Razanajatovo Rahombanjanahary et al., 2020 ; Yun, Choi, and Lee 2020 ).…”

Section: Resultssupporting

confidence: 92%

The spatial-temporal dynamics of respiratory syncytial virus infections across the east–west coasts of Australia during 2016–17

Robertson

¹

,

Eden

²

,

Levy

³

et al. 2021

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Respiratory syncytial virus (RSV) is an important human respiratory pathogen. In temperate regions a distinct seasonality is observed, where peaks of infections typically occur in early winter, often preceding the annual influenza season. Infections are associated with high rates of morbidity and mortality, and in some populations exceeds that of influenza. Two subtypes, RSV-A and RSV-B, have been described, and molecular epidemiological studies have shown that both viruses mostly co-circulate. This trend also appears to be the case for Australia, however previous genomic studies have been limited to cases from one Eastern state - New South Wales. As such, the broader spatial patterns and viral traffic networks across the continent are not known. Here, we conducted a whole genome study of RSV comparing strains across eastern and western Australia during the period January 2016 to June 2017. In total, 96 new RSV genomes were sequenced, compiled with previously generated data, and examined using a phylodynamic approach. This analysis revealed that both RSV-A and RSV-B strains were circulating, and each subtype was dominated by a single genotype, RSV-A/ON1-like and RSV-B/BA10-like viruses. Some geographical clustering was evident in strains from both states with multiple distinct sub-lineages observed and relatively low mixing across jurisdictions suggesting that endemic transmission was likely seeded from imported, unsampled locations. Overall, the RSV phylogenies reflected a complex pattern of interactions across multiple epidemiological scales from fluid virus traffic across global and regional networks to fine-scale local transmission events.

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“…Specifically, with this lab testing data a transition from RSV-B to RSV-A was observed between the 2016 and 2017 seasons in WA (data not shown). These results are consistent with other molecular epidemiological studies globally that show both RSV-A and RSV-B subtypes co-circulate with shifting predominance across seasons (Di Giallonardo et al, 2018; Luo et al, 2020; Razanajatovo Rahombanjanahary et al, 2020; Yun et al, 2020)…”

Section: Resultssupporting

confidence: 92%

“…Specifically, a transition from RSV-B to RSV-A was observed between the 2016 and seasons in WA (data not shown). These results are consistent with other molecular epidemiological studies globally that show both RSV-A and RSV-B subtypes co-circulate with shifting predominance across seasons [7,[31][32][33] were generated in a previous study [7].…”

Section: Resultssupporting

confidence: 92%

The spatial-temporal dynamics of respiratory syncytial virus infections across the east-west coasts of Australia during 2016-17

Robertson

¹

,

Eden

²

,

Levy

³

et al. 2020

Preprint

View full text Add to dashboard Cite

Respiratory syncytial virus (RSV) is an important human respiratory pathogen. In temperate regions a distinct seasonality is observed, where peaks of infections typically occur in early winter, often preceding the annual influenza season. Infections are associated with high rates of morbidity and mortality, and in some populations exceeds that of influenza. Two subtypes, RSV-A and RSV-B, have been described, and molecular epidemiological studies have shown that both viruses mostly co-circulate. This trend also appears to be the case for Australia, however previous genomic studies have been limited to cases from one Eastern state - New South Wales. As such, the broader spatial patterns and viral traffic networks across the continent are not known. Here, we conducted a whole genome study of RSV comparing strains across eastern and western Australia during the period January 2016 to June 2017. In total, 96 new RSV genomes were sequenced, compiled with previously generated data, and examined using a phylodynamic approach. This analysis revealed that both RSV-A and RSV-B strains were circulating, and each subtype was dominated by a single genotype, RSV-A/ON1-like and RSV-B/BA10-like viruses. Some geographical clustering was evident in strains from both states with multiple distinct sub-lineages observed and relatively low mixing across jurisdictions suggesting that endemic transmission was likely seeded from imported, unsampled locations. Overall, the RSV phylogenies reflected a complex pattern of interactions across multiple epidemiological scales from fluid virus traffic across global and regional networks to fine-scale local transmission events.

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“…Each CDC panel consisted of 20 freeze‐dried samples prepared from RSV‐infected or uninfected cell cultures (Table 1). Four RSV strains were used 15353_ON1 (RSV A) 15,16 RSV Long strain (RSV A), 17 53530_BA (RSV B), 18 and 209_GB3 (RSV B) 19,20 . Viruses were grown using Hep‐2 monolayer with 10% MEM + 20 mM HEPES and inactivated by gamma‐irradiation.…”

Section: Methodsmentioning

confidence: 99%

“…Each CDC panel consisted of 20 freeze-dried samples prepared from RSV-infected or uninfected cell cultures (Table 1). Four RSV strains were used 15353_ON1 (RSV A) 15,16 RSV Long strain (RSV A), 17…”

Section: Preparation and Composition Of The Cdc Rsv Eqa Panelmentioning

confidence: 99%

Results from the WHO external quality assessment for the respiratory syncytial virus pilot, 2016‐17

Jackson

¹

,

Peret

²

,

Ziegler

³

et al. 2020

Influenza Resp Viruses

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Molecular epidemiology of respiratory syncytial virus for 28 consecutive seasons (1990-2018) and genetic variability of the duplication region in the G gene of genotypes ON1 and BA in South Korea

Cited by 26 publications

References 31 publications

The spatial-temporal dynamics of respiratory syncytial virus infections across the east–west coasts of Australia during 2016–17

The spatial-temporal dynamics of respiratory syncytial virus infections across the east–west coasts of Australia during 2016–17

The spatial-temporal dynamics of respiratory syncytial virus infections across the east-west coasts of Australia during 2016-17

Results from the WHO external quality assessment for the respiratory syncytial virus pilot, 2016‐17

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