Molecular epidemiology of recent United Kingdom isolates of<i>Neisseria meningitidis</i>serogroup C

Yakubu, D. E.; Abadi, F. J. R.; Pennington, T. H.

doi:10.1017/s0950268800051463

Cited by 14 publications

(12 citation statements)

References 32 publications

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“…Knowledge of the circulation of phenotypes with a clonal structure in the meningococcal population of a country, even during endemic periods, may be useful as part of a routine surveillance of disease‐associated isolates. In fact, the combination of serologic typing with a relatively simple genomic typing technique such as PFGE, as shown also in other studies [17,18], allowed us to monitor both old and new features in the meningococcal population.…”

Section: Discussionmentioning

confidence: 86%

Monitoring the genotype of meningococcal strains during an endemic period

Mastrantonio¹,

Stefanelli²,

Conti³

et al. 1999

Clinical Microbiology and Infection

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Obioctiie:To monitor the circulation of clones among the most frequent phenotypes of Neisseria meningitidis isolated from sporadic cases in Italy in recent years.Method.: N. meningitidis strains sent to the reference laboratory for meningococcal meningitis of the lstituto Superiore di Sanitir were typed for serogroup, serotype and serosubtype, and chromosomal DNAs were analyled by pulsed-field gel electrophoresis. k s u k Of 185 strains sent during the years 1995-97,75% belonged to serogroup B. Although many different serotypes and subtypes were present, the most common combinations were B14:P1.13, B:4:P1.13, B15P1.7 and C2bP1.2. PFGE analysis of patterns obtained by using Nhel and Bg/H enzymes showed a quite stable genomic structure, except in the phenotype B:4:P1.13.Conclusions: On the basis of serotype and serosubtype analysis of recently isolated meningococci, a great variety of phenotypes has been circulating during these endemic years. A clonal structure in strains belonging to the same phenotypes was found, suggesting that some favorable conditions have led to stability of these highly transmissible strains.

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Section: Discussionmentioning

confidence: 86%

Monitoring the genotype of meningococcal strains during an endemic period

Mastrantonio¹,

Stefanelli²,

Conti³

et al. 1999

Clinical Microbiology and Infection

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“…Due to that diversity and to the fact that in the United States it is mainly associated with sporadic meningococcal disease, PFGE analysis would not be particularly useful for epidemiological purposes. PFGE has been used as a tool to molecularly characterize NMSC isolates from sporadic cases of meningococcal disease and in NMSC outbreak investigations (3,7,8,18,28 We chose four well-described NMSC outbreaks occurring from 1993 to 1995 in Texas, New Mexico, Arizona, and California (15,22,26) to systematically evaluate the potential of PFGE to identify outbreak-associated isolates. We also included in the study 26 NMSC isolates, representing sporadic disease in the United States, collected through the active laboratory-based surveillance program from 1989 to 1996.…”

mentioning

confidence: 99%

Evaluation of Pulsed-Field Gel Electrophoresis in Epidemiological Investigations of Meningococcal Disease Outbreaks Caused by Neisseria meningitidis Serogroup C

et al. 2001

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Since 1990, the frequency of Neisseria meningitidis serogroup C (NMSC) outbreaks in the United States has increased. Based on multilocus enzyme electrophoresis (MEE), the current molecular subtyping standard, most of the NMSC outbreaks have been caused by isolates of several closely related electrophoretic types (ETs) within the ET-37 complex. We chose 66 isolates from four well-described NMSC outbreaks that occurred in the United States from 1993 to 1995 to evaluate the potential of pulsed-field gel electrophoresis (PFGE) to identify outbreak-related isolates specific for each of the four outbreaks and to differentiate between them and 50 sporadic isolates collected during the outbreak investigations or through active laboratory-based surveillance from 1989 to 1996. We tested all isolates collected during the outbreak investigations by four other molecular subtyping methods: MEE, ribotyping (ClaI), random amplified polymorphic DNA assay (two primers), and serotyping and serosubtyping. Among the 116 isolates, we observed 11 clusters of 39 NheI PFGE patterns. Excellent correlation between the PFGE and the epidemiological data was observed, with an overall sensitivity of 85% and specificity of 71% at the 95% pattern relatedness breakpoint using either 1.5 or 1.0% tolerance. For all four analyzed outbreaks, PFGE would have given public health officials additional support in declaring an outbreak and making appropriate public health decisions.

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“…3 1 (0.08%) strain was multiply resistant to penicillin and rifampicin. 4 1 (0.08%) strain was multiply resistant to penicillin, rifampicin and sulphadiazine.…”

Section: Resultsmentioning

confidence: 99%

“…One hundred and twenty-one strains of N. meningitidis isolated in different locations in the United Kingdom have been described in detail before [2,4,8]. Four mutants highly resistant to rifampicin [minimum inhibitory concentrations (MIC 90 ) 1 256 Ìg/ml] generated in a previous study [9] as well as control strains were included in this study.…”

Section: Bacterial Strainsmentioning

confidence: 99%

“…Although of low prevalence (3%) [1], penicillin-resistant [2] and rifampicin-resistant [3,4] meningococci have been isolated in the United Kingdom. Emergence of meningococcal strains that are resistant to first choice antimicrobial agents not only pose an increasing problem in the clinical management of meningococcal infections, but also stress the need for constant monitoring of resistance and of the development of new agents for prophylactic and therapeutic use.…”

Section: Introductionmentioning

confidence: 99%

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In vitro Activities of Meropenem and Other Antimicrobial Agents against British Meningococcal Isolates

Abadi

Yakubu²,

Pennington³

1999

Chemotherapy

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The in vitro activity of meropenem was compared with those of penicillin, ampicillin, cefuroxime, cetriaxone, cefotaxime, rifampicin, chloramphenicol, sulphadiazine and ciprofloxacin against 121 British meningococcal isolates by a microdilution method in Mueller-Hinton broth and by the E test. All meningococcal strains were susceptible to the agents except for ampicillin (88.4%), penicillin (88.4%), sulphadiazine (57.9%) and rifampicin (95%). The emergence of resistance problems among meningococcal isolates stresses the need for their constant monitoring and of the development of new agents. In this study we have shown that meropenem is highly active in vitro against Neisseria meningitidis. Recent studies have indicated that meropenem is highly active clinically and bacteriologically in the treatment of bacterial meningitis. Thus, the potentials of meropenem as meningococcal prophylactic and therapeutic agent needs to be fully evaluated.

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Molecular epidemiology of recent United Kingdom isolates ofNeisseria meningitidisserogroup C

Cited by 14 publications

References 32 publications

Monitoring the genotype of meningococcal strains during an endemic period

Monitoring the genotype of meningococcal strains during an endemic period

Evaluation of Pulsed-Field Gel Electrophoresis in Epidemiological Investigations of Meningococcal Disease Outbreaks Caused by Neisseria meningitidis Serogroup C

In vitro Activities of Meropenem and Other Antimicrobial Agents against British Meningococcal Isolates

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