Molecular Epidemiology of Plasmid-Mediated Types 1 and 3 Fimbriae Associated with Biofilm Formation in Multidrug Resistant Escherichia coli from Diseased Food Animals in Guangdong, China

Guo, Wenying; Zhang, Hui; Cheng, Ming Fang; Huang, Minrui; Li, Qian; Jiang, Yuwei; Zhang, Jixing; Sun, Ruan-Yang; Wang, Min-Ge; Liao, Xiao‐Ping; Liu, Ya-Hong; Sun, Jian; Fang, Liang‐Xing

doi:10.1128/spectrum.02503-21

Cited by 4 publications

(3 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, more than 50% of the strains co-carried bla CTX–M , such a high prevalence of bla CTX–M showed that most of these strains belonged to MDR strains. Meanwhile, the co-carriage of 16s RNA methylase, PMQRs, and fosfomycin genes suggested that the mcr -positive strains were also reservoirs of numerous resistance genes ( Guo et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

“…It can easily acquire multi-drug resistance, owing to its ability to accumulate multiple resistance genes, primarily through horizontal gene transfer of mobile elements. Currently, its acquisition of genes encoding extended-spectrum β-lactamases (ESBLs), carbapenem-hydrolyzing ß-lactamase (CHßls), 16S rRNA methylases, plasmid-mediated quinolone resistance (PMQR), and plasmid-mediated glutathione S-transferase (PMGST) is of great clinical concern ( Simoni et al, 2021 ; Yin et al, 2021 ; Guo et al, 2022 ), accelerating the evolution of drug resistance, making colistin the last-resort antibiotic for treating infections caused by multi-drug resistant bacteria. The use of colistin in livestock as a growth promoter has been banned in China ( Wang et al, 2020 ) due to increasing polymyxin resistance.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Epidemiological and genomic characteristics of global mcr-positive Escherichia coli isolates

et al. 2023

View full text Add to dashboard Cite

ObjectiveThe worldwide dissemination of colistin-resistant Escherichia coli (E. coli) endangers public health. This study aimed to better understand the global genomic epidemiology of E. coli isolates carrying mobilized colistin resistance (mcr) genes, providing information to assist in infection and prevention.MethodsEscherichia coli genomes were downloaded from NCBI, and mcr was detected using BLASTP. Per software was used to extract information on hosts, resources, collection data, and countries of origin from GenBank. Sequence types (STs), prevalence of plasmids, antimicrobial resistance genes (ARG), and virulence factors (VF) in these genomes were analyzed. Statistical analyses were performed to assess the relationships between mcr, ARGs, plasmids, and STs.ResultsIn total, 778 mcr-positive isolates were identified. Four mcr variants were detected, with mcr-1 (86.1%) being the most widespread, followed by mcr-9 (5.7%), mcr-5 (4.4%), and mcr-3 (3.0%). Multiple ARGs were identified, with blaCTX–M (53.3%), fosA (28.8%), qnr (26.1%), blaNDM (19.8%), and aac (6’)-Ib-cr (14.5%) being the most common. Overall, 239 distinct STs were identified, of which ST10 (13.8%) was the most prevalent. A total of 113 different VFs were found, terC (99.9%) and gad (83.0%) were most frequently detected. Twenty types of plasmids were identified; IncFIB (64.1%), IncX (42.3%), and IncX (42.3%) were the most common replicons. IncI2 and IncX4 were frequently detected in mcr-1-positive isolates, whereas IncFII, IncI1-I, and IncHI2 were dominant plasmids in mcr-3, mcr-5, and mcr-9-positive isolates, respectively. A higher frequency of ARGs and VFs was observed among ST156 and ST131 isolates.ConclusionOur data indicated that more than half of the mcr-positive E. coli strains carried endemic ARGs and VFs. ST10 and ST156 isolates deserved further attention, given the rapid transmission of ST10 and the convergence of ARGs and VFs in ST156.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Epidemiological and genomic characteristics of global mcr-positive Escherichia coli isolates

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Moreover, bacterial biofilms on the surfaces of indwelling medical and dental devices make the treatment of infections increasingly difficult ( Linklater et al, 2021 ). Biofilms formation within the host have been implicated in diseases such as cystic fibrosis, urinary tract infections, and endocarditis ( Guo et al, 2022 ). The negative impacts of microbial biofilms are incalculable, resulting in an annual loss of millions of US dollars ( Cattò and Cappitelli, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

The combination of allicin with domiphen is effective against microbial biofilm formation

Li,

Wang,

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

BackgroundMicroorganisms in biofilms are particularly difficult to control because of their increased survival and antibiotic resistance. Allicin and domiphen were employed to inhibit the microbial growth and biofilm formation of Staphylococcus aureus, Escherichia coli, and Candida albicans strains.MethodsBroth microdilution method and checkerboard assay were conducted to determine the efficacy of allicin combined with domiphen against S. aureus, E. coli, and C. albicans. Microbial biofilm formation was measured using the crystal violet staining method and fluorescence microscopy. And the total viable count of the biofilm cells on material surface after the treatment with antimicrobial reagents was calculated with the plate count technique.ResultsThe two drugs showed synergistic effects against the pathogens with a fractional bactericidal concentration of less than 0.38. The combination of 64 μg/mL allicin with 1 μg/mL domiphen dispersed over 50% of the biofilm mass of S. aureus, E. coli, and C. albicans. In addition, the drug combination reduced the total viable counts of E. coli and C. albicans biofilm cells on stainless steel and polyethylene surfaces by more than 102 CFU/mL.ConclusionThe combination of allicin and domiphen is an effective strategy for efficiently decreasing biofilms formation on various industrial materials surfaces.

show abstract

Computational Systems and Network Biology Perspective: Understanding Klebsiella pneumoniae Infection Mechanisms

Mazaya,

Panjaitan,

Hayati

2024

The Microbe

View full text Add to dashboard Cite

Molecular Epidemiology of Plasmid-Mediated Types 1 and 3 Fimbriae Associated with Biofilm Formation in Multidrug Resistant Escherichia coli from Diseased Food Animals in Guangdong, China

Cited by 4 publications

References 46 publications

Epidemiological and genomic characteristics of global mcr-positive Escherichia coli isolates

Epidemiological and genomic characteristics of global mcr-positive Escherichia coli isolates

The combination of allicin with domiphen is effective against microbial biofilm formation

Computational Systems and Network Biology Perspective: Understanding Klebsiella pneumoniae Infection Mechanisms

Contact Info

Product

Resources

About