Molecular epidemiology of norovirus in South Korea

Lee, Soo Kon; Cho, Han Gil; Paik, Soon Young

doi:10.5483/bmbrep.2015.48.2.254

Cited by 13 publications

(12 citation statements)

References 45 publications

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“…Our detection of the genogroups I and II (GI and GII) of NoV agree with previous reports from different water matrices in the same region and worldwide (40)(41)(42).…”

Section: Discussionsupporting

confidence: 93%

Enteric Viruses in Surface Waters from Argentina: Molecular and Viable-Virus Detection

Masachessi

Pisano

Prez

et al. 2018

Appl Environ Microbiol

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Water resources contaminated with wastewater are an important source for the dissemination of enteric viruses with an impact on the health of the population. The aim of the study was to assess the viral contamination of freshwater from a dam in Argentina by using infectious enterovirus detection, viral RNA amplification, and a genetic characterization of five enteric viruses associated with diarrhea and hepatitis. Enterovirus infectivity (iEV) was evaluated by cell culture and direct immunofluorescence. The detection of the viral genome of rotavirus (RV), human astrovirus (HAstV), norovirus (NoV), hepatitis A virus (HAV), and hepatitis E virus (HEV) was performed by reverse transcriptase PCR (RT-PCR). A total of 48 water samples from 4 monitoring points on the body of the dam from January to December 2012 and 66 water samples from 3 tourist beaches on the edge of the dam from October 2013 to October 2015 were collected monthly. During the first period, the overall viral frequency detection was 52.1% for group A RV, 50% for HAstV, 60.4% for NoV, 22.9% for HAV, 2.1% for HEV, and 64.6% for iEV. The overall frequency detection for the second sampling was 18.2% for RV and HAstV, 31.8% for NoV, 7.57% for HEV, and 66.7% for iEV. There was no detection of HAV during this period. The genotypes and genogroups detected through the study correlated with the most common genomic variants associated with human gastrointestinal and hepatitis illnesses. The results obtained could alert the health systems and environmental sanitation to make decisions for viral control and prevention in our environment. The study shows the impact of anthropic contamination of one of the most important tourist water resources in Argentina. This course of recreational water would be a favorable scenario for infection, as well as a reservoir for the enteric viruses, creating a risk for the population exposed to these waters. The results obtained could alert the health systems and environmental sanitation to make decisions for the control and prevention of viral diseases in this environment.

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“…Our detection of the genogroups I and II (GI and GII) of NoV agree with previous reports from different water matrices in the same region and worldwide (40)(41)(42).…”

Section: Discussionsupporting

confidence: 93%

Enteric Viruses in Surface Waters from Argentina: Molecular and Viable-Virus Detection

Masachessi

Pisano

Prez

et al. 2018

Appl Environ Microbiol

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“…Furthermore, vaccination can decreased the secondary spreading of the virus. Currently, norovirus is the most common pathogen of viral gastroenteritis in South Korea 14,15) . Kim et al 19) reported the common pathogens of viral gastroenteritis to include norovirus (63.0% of cases) and rotavirus (8.7%), followed by enteric adenovirus (8.7%), and astrovirus (2.8% of cases).…”

Section: Seizure Characteristics In Children With Practical Status Epmentioning

confidence: 99%

“…Rotavirus was the most commonly detected pathogen in CwG [3][4][5] . However, after the introduction of a rotavirus vaccine, norovirus has become the most common cause of viral gastroenteritis [13][14][15][16] , even in cases of CwG in infants and children,…”

Section: Introductionmentioning

confidence: 99%

Convulsions with Noroviral Gastroenteritis in Children at a Single Center in Korea

강수연¹,

이현주²,

김신혜³

2018

Child Neurol

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Viral gastroenteritis is a common disease in infants and children. Seizures can be associated with viral gastroenteritis as benign convulsions with mild gastroenteritis (CwG). After the development of the rotavirus vaccination, norovirus has become inreasingly significant in children. We retrospectively analyzed the clinical features in a pediatric population presenting with seizures and confirmed enteral viral infections, especially norovirus infections. Methods: We retrospectively reviewed the medical records of pediatric patients aged <15 years admitted due to convulsions and gastroenteritis to the Department of Pediatrics of Myongji Hospital between July 2014 and June 2016. Results: A total of 46 patients (24 male and 22 female) were included. Norovirus was detected in 21 (45.7%) patients, adenovirus in three (6.5%), rotavirus in two (4.3%), astrovirus in one (2.2%), and none of agents were detected in 19 (41.3%) patients. Patients in the norovirus gastroenteritis (NGE) group had a higher incidence of diarrhea than that among in the non-norovirus gastroenteritis (NNGE) group (61.9% vs 28.0%; P<0.05). Twelve patients experienced status epilepticus, including five (23.8%) in the NGE group and seven (33.0%) in the NNGE group (P=0.837). Seizures were effectively terminated by intravenous benzodiazepines in 8 (66.7%) of 12 patients. Additional long-acting antiepileptic drugs such as fosphenytoin or levetiracetam were required in three (25%) of 12 patients. Conclusion: Patients with CwG with and without noroviurs infection did not differ in terms of clinical features. However, status epilepticus was not uncommon among patients with CwG by definition.

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“…Fresh lettuce and frozen berries are problematic because they can harbor HAV [4,[28][29][30]. In Korea, a less fermented vegetable product is infrequently associated with acute gastroenteritis in humans, likely due to the use of untreated groundwater [9]. Focusing on these foods, we evaluated whether our multiplex PCR method could be applied.…”

Section: Tests Of the Artificially Contaminated Water And Food Samplesmentioning

confidence: 99%

“…Major viruses causing gastroenteritis in humans through food consumption include the norovirus (NoV) GI and GII genogroups, hepatitis a virus (HAV), astrovirus (AstV), and rotavirus (RoV) [2,3]. Representative viralcontaminated foods include water, shellfish (oysters), and fresh vegetables [4][5][6][7][8][9][10]. Prior reports of viral outbreaks from foods have inspired many additional studies related to viral contamination in foods [4,7,11,12].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Detection of Four Foodborne Viruses in Food Samples Using a One-Step Multiplex Reverse Transcription PCR

Lee

Kim

et al. 2018

Journal of Microbiology and Biotechnology

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A one-step multiplex reverse transcription PCR (RT-PCR) method comprising six primer sets (for the detection of norovirus GI and GII, hepatitis A virus, rotavirus, and astrovirus) was developed to simultaneously detect four kinds of pathogenic viruses. The size of the PCR products for norovirus GI and GII, hepatitis A virus (VP3/VP1 and P2A regions), rotavirus, and astrovirus were 330, 164, 244, 198, 629, and 449 bp, respectively. The RT-PCR with the six primer sets showed specificity for the pathogenic viruses. The detection limit of the developed multiplex RT-PCR, as evaluated using serially diluted viral RNAs, was comparable to that of one-step single RT-PCR. Moreover, this multiplex RT-PCR was evaluated using food samples such as water, oysters, lettuce, and vegetable product. These food samples were artificially spiked with the four kinds of viruses in diverse combinations, and the spiked viruses in all food samples were detected successfully.

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Molecular epidemiology of norovirus in South Korea

Cited by 13 publications

References 45 publications

Enteric Viruses in Surface Waters from Argentina: Molecular and Viable-Virus Detection

Enteric Viruses in Surface Waters from Argentina: Molecular and Viable-Virus Detection

Convulsions with Noroviral Gastroenteritis in Children at a Single Center in Korea

Simultaneous Detection of Four Foodborne Viruses in Food Samples Using a One-Step Multiplex Reverse Transcription PCR

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