Molecular epidemiology of human Blastocystis in a village in Yunnan province, China

Li, Lanhua; Zhou, Xiao‐Nong; Du, Zhenhong; Wang, Xuezhong; Wang, Libo; Jiang, Jin-Yong; Yoshikawa, Hideki; Steinmann, Peter; Utzinger, Jürg; Wu, Zhiliang; Chen, Jiaxu; Chen, Shaohong; Zhang, Ling

doi:10.1016/j.parint.2007.06.001

Cited by 133 publications

(98 citation statements)

References 27 publications

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“…The results are similar to some other studies performed worldwide (14,15,21,(36)(37)(38). The frequency of subtype SB83 was more than subtypes SB227 and SB340 that is similar to some other researches (5,17,20). The subtypes SB337, SB336, SB332 and SB155 were not detected.…”

Section: Discussionsupporting

confidence: 86%

“…Furthermore, 14.6 % of the persons were infected by genotypes SB83 and SB227, simultaneously. In some studies, the rate of mixed infections has been observed from 1.1 to 14.3 (5,19,21,39). The frequency of mixed infections with subtypes SB83 and SB340 (5), with SB340 and SB227 (5), and with SB227 and SB336 (39) Sardarian K et al…”

Section: Discussionmentioning

confidence: 99%

“…Infections with the organism are spread worldwide and it is often the most frequently isolated protozoan in parasitological surveys (2)(3)(4). In developing countries, B. hominis has higher prevalence (30-50 %) in comparison with developed countries (1.5-10 %) (5,6). The pathogenicity of B. hominis still has been debated.…”

Section: Introductionmentioning

confidence: 99%

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A Study of The Genetic Variability of Blastocystis hominis Isolates in Hamadan, West of Iran

Sardarian

Hajilooi

Maghsood

et al. 2012

Jundishapur J Microbiol

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Blastocystis hominis is a common protozoan parasite in the human and many other animals intestine. The parasite pathogenicity is still controversial. A few studies have been conducted on prevalence of B. hominis subtypes in Iran. The results of present study improve our knowledge on B. hominis subtypes in Iran and therefore we can do more investigations on pathogenicity potential of these subtypes in the future. Background:Blastocystis is a common protozoan parasite in mammals, birds, amphibians, reptiles, fish, arthropods, and annelids. This parasite has some subtypes, which pathogenicity status of them still remained controversial. Some of Blastocystis subtypes are potentially pathogenic to human. Objectives: This study has identified the Blastocystis hominis subtypes and their prevalence rates in Hamadan. Materials and Methods: During two months of summer 2011, a total number of 250 human fecal samples referred for parasitology examination to Beasat Hospital and a few clinical laboratories of Hamadan city were collected. The samples were examined by direct method and formalin-ether. 41 samples exhibited positive results for B. hominis thereby were cultured in Locke-egg medium. After the growth and in order to genotype identification, B. hominis isolates were amplified by PCR, using seven pairs of sequencestagged site primers. Results: In this study, three subtypes of B. hominis consisted of one [SB83], two [SB340] and three [SB227] were identified. The most dominant genotype was SB83 with 56.1 % frequency. The prevalence rate of genotype SB227 and SB340 were 22 % and 7.3 %, respectively. Coexistence of genotypes SB83 and SB227 was detected in 14.6 % of positive cases. Conclusions: This is the first study in Hamadan on genotyping of B. hominis, which may trigger other epidemiologic and zoonotic studies on different subtypes and hence control clinical manifestations of infection.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

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A Study of The Genetic Variability of Blastocystis hominis Isolates in Hamadan, West of Iran

Sardarian

Hajilooi

Maghsood

et al. 2012

Jundishapur J Microbiol

View full text Add to dashboard Cite

show abstract

“…Furthermore, 14.6% of the persons were infected by genotypes SB83 and SB227, simultaneously. In some studies, the rate of mixed infections has been observed from 1.1 to 14.3 (5,19,21,39). The frequency of mixed infections with subtypes SB83 and SB340 (5), with SB340 and SB227 (5), and with SB227 and SB336 (39) was low.…”

Section: Discussionmentioning

confidence: 99%

A Study of The Genetic Variability of Blastocystis hominis Isolates in Hamadan, West of Iran

Sardarian

Hajilooi

Maghsood

et al. 2012

Jundishapur J Microbiol

View full text Add to dashboard Cite

Blastocystis hominis is a common protozoan parasite in the human and many other animals intestine. the parasite pathogenicity is still controversial. A few studies have been conducted on prevalence of B.hominis subtypes in Iran. The results of present study improve our knowledge on B. hominis subtypes in Iran and therefore we can do more investigations on pathogenicity potential of these subtypes in the future. Background:Blastocystis is a common protozoan parasite in mammals, birds, amphibians, reptiles, fish, arthropods, and annelids. This parasite has some subtypes, which pathogenicity status of them still remained controversial. Some of Blastocystis subtypes are potentially pathogenic to human. Objectives: This study has identified the B. hominis subtypes and their prevalence rates in Hamadan. Patients and Methods: During two months of summer 2011, a total number of 250 human fecal samples referred for parasitology examination to Beasat Hospital and a few clinical laboratories of Hamadan city were collected. The samples were examined by direct method and formalin-ether. 41 samples exhibited positive results for B. hominis thereby were cultured in Locke-egg medium. After the growth and in order to genotype identification, B. hominis isolates were amplified by PCR, using seven pairs of sequencestagged site primers. . Results: In this study, three subtypes of B. hominis consisted of one [SB83], two [SB340] and three [SB227] were identified. The most dominant genotype was SB83 with 56.1% frequency. The prevalence rate of genotype SB227 and SB340 were 22% and 7.3%, respectively. Coexistence of genotypes SB83 and SB227 was detected in 14.6% of positive cases. Conclusions: This is the first study in Hamadan on genotyping of B. hominis, which may trigger other epidemiologic and zoonotic studies on different subtypes and hence control clinical manifestations of infection.

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“…ST3 was the predominant subtype in 70.5% of the positive population, followed by ST1 with 20.5%. The significance of hygienic practices was shown to have an effect on the type of infection with ST3 corresponding to the drinking of unboiled water and ST1 relating to the consumption of raw water plants [39].…”

Section: Epidemiologymentioning

confidence: 99%

Update on the Molecular Epidemiology and Diagnostic Tools for Blastocystis sp

Roberts¹,

Stark²,

Harkness³

et al. 2014

J Med Microb Diagn

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Blastocystis is the most common enteric protist found in humans. Due to the recent advancements in molecular technologies, up to 17 subtypes have been identified from humans and animals. Molecular epidemiological studies have shown the large range of subtype (ST) distribution within geographical locations with ST1-9 being identified in humans. ST3 has been identified as the predominant subtype in most epidemiological studies with a considerable absence of ST4 noted in Africa and the Middle East compared to Europe and Australia where this subtype is fairly common. This review summarises the molecular tools used for diagnosis and speciation of Blastocystis and comments on advancements in the area over the last 15 years and what future trends may be. This review also describes the geographical distribution of Blastocystis and comments on possible intra-subtype evolutionary relationships.

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Molecular epidemiology of human Blastocystis in a village in Yunnan province, China

Cited by 133 publications

References 27 publications

A Study of The Genetic Variability of Blastocystis hominis Isolates in Hamadan, West of Iran

A Study of The Genetic Variability of Blastocystis hominis Isolates in Hamadan, West of Iran

A Study of The Genetic Variability of Blastocystis hominis Isolates in Hamadan, West of Iran

Update on the Molecular Epidemiology and Diagnostic Tools for Blastocystis sp

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