Molecular Epidemiology of Hepatitis A Virus in the South-East Area of Gyeonggi-do in Korea

Song, Han Ul; Hwang, Seong Gyu; Kwon, Chang-Il; Lee, Ji Eun; Ko, Kwang Hyun; Hong, Sung Pyo; Park, Phil Won; Rim, Kyu Sung

doi:10.3349/ymj.2009.50.4.512

Cited by 13 publications

(5 citation statements)

References 21 publications

(35 reference statements)

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“…Until 2004, subgenotype IA was the most prevalent in the Republic of Korea [25]. However, studies conducted between 2004 and 2008 reported the cocirculation of the 2 prevalent subgenotypes IA and IIIA [22, 25, 26]. Subgenotype IIIA has been the predominant subgenotype since 2008, according to a previous study [22, 26].…”

Section: Introductionmentioning

confidence: 99%

Full-Length Genomic Sequence of Subgenotype IIIA Hepatitis A Virus Isolate in Republic of Korea

Lee

Kang

et al. 2013

BioMed Research International

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Hepatitis A virus is known to cause acute hepatitis and has significant implications for public health throughout the world. In the Republic of Korea, the number of patients with hepatitis A virus infection has been increasing rapidly since 2006. In this study, the Kor-HAV-F strain was identified as subgenotype IIIA by RT-PCR, and its identity was confirmed by nucleotide sequencing and alignment analysis. Moreover, detailed phylogenetic analysis indicated that the Kor-HAV-F strain clustered into subgenotype IIIA, including strains isolated in Japan, Norway, and India. The entire amino acid sequence of the VP1 and 2A regions was compared with that of the reference strains isolated in various countries. We found 2 amino acid changes (T168A and L96P, resp.) in the VP1 and 2A regions, which had not been found in any other hepatitis A virus strain. To our knowledge, this study is the first to report the full-length sequence of a hepatitis A virus isolated in the Republic of Korea.

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Section: Introductionmentioning

confidence: 99%

Full-Length Genomic Sequence of Subgenotype IIIA Hepatitis A Virus Isolate in Republic of Korea

Lee

Kang

et al. 2013

BioMed Research International

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“…Until early 2000, almost all isolates tested had been identified as genotype IA [Byun et al, 2001]. However, studies conducted from 2004 to 2008 reported the co-circulation of the two prevalent genotypes IA and IIIA [Yoo et al, 2008;Yun et al, 2008;Park et al, 2009;Song et al, 2009]. More recent reports showed that genotype IIIA was the predominant serotype since 2008 Yoon et al, 2009].…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of disease severity between genotypes IA and IIIA of hepatitis A virus

Yoon

Yeon

Kim

et al. 2011

Journal of Medical Virology

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Although hepatitis A is a major health problem worldwide, it has not yet been clarified whether or not viral factors affect the clinical characteristics. This study aimed to investigate if a genotype of hepatitis A virus (HAV) affects disease severity among adolescent and adult populations. Clinical data and specimens were collected from patients ≥16-years-of-age with acute hepatitis A at two university hospitals in Korea during the two study periods: 1998 and 1999 (n = 45), and 2009 (n = 66). Nucleotide sequencing of the complete VP1 region of the HAV isolates was performed for phylogenetic analysis and genotyping. Clinical parameters related to disease severity were compared by HAV genotype to determine its clinical relevance. Of the 87 patients, 47 were male and the mean age was 29.8 ± 8.1 years. The genotype IIIA (93.0%, 53/57) was predominant in the year 2009, whereas IA (93.3%, 28/30) was the major genotype in 1998 and 1999. When comparing disease severity between the two HAV genotypes, the patients with genotype IIIA were older and had higher alanine aminotransferase (ALT) levels, prolonged prothrombin times and lower serum albumin levels. In a multivariate logistic regression model, higher ALT levels ≥ 1,000 IU/L (odds ratio [OR] 11.7, 95% confidence interval [CI] 2.5-54.0) and longer hospitalization (OR 22.49, 95%CI 4.6-132.5) were associated independently with genotype IIIA. In conclusion, this study indicates that HAV genotype might be one of the viral factors responsible for the disease severity of hepatitis A.

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“…Nevertheless, cross-reactivity could be only definitively excluded with the entire genome sequencing for the tested samples. However, co-circulation of the subgenotypes IA and IIIA has been reported in India [ 33 ] and of IA, IB and IIIA in Korea [ 34 ]. Co-circulation of the subgenotypes IA and IB in South Africa, South America, Europe and the US and the existence of recombination events between subgenotypes have also been observed [ 35 - 37 ].…”

Section: Discussionmentioning

confidence: 99%

Hepatitis A virus subgenotyping based on RT-qPCR assays

et al. 2014

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BackgroundThe hepatitis A virus (HAV) is the most frequent cause of viral hepatitis worldwide and is recognized as one of the most widespread foodborne pathogens. HAV genotypes and subtypes differ in their geographic distribution and the incidence of HAV infection varies considerably among countries, and is particularly high in areas with poor sanitation and hygiene. Phylogenetic analyses are traditionally used in clinical microbiology for tracing the geographic origin of HAV strains. In food microbiology, this approach is complicated by the low contamination levels of food samples. To date, real-time reverse-transcription PCR has been one of the most promising detection methods due to its sensitivity, specificity and ability to deliver quantitative data in food samples, but it does not provide HAV subtyping information.ResultsSix subtype-specific RT-qPCR assays were developed for human HAV. The limit of detection of HAV was 50 genome copies/assay for subtype IIB, 500 genome copies assay for IA, IB, IIA and IIIB and 5000 genome copies/assay for IIIA. The specificity of the assays was evaluated by testing reference isolates and in vitro HAV RNA transcripts. No significant cross reactivity was observed. Subtyping results concordant with sequencing analysis were obtained from 34/35 clinical samples. Co-infection with a minor strain of a different subtype was suggested in 5 cases and a recombinant event in one case.ConclusionsThese RT-qPCR assays may be particularly useful for accurately tracing HAV in low-level contaminated samples such as food matrices but also to allow co-infection identification in human samples.

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Molecular Epidemiology of Hepatitis A Virus in the South-East Area of Gyeonggi-do in Korea

Cited by 13 publications

References 21 publications

Full-Length Genomic Sequence of Subgenotype IIIA Hepatitis A Virus Isolate in Republic of Korea

Full-Length Genomic Sequence of Subgenotype IIIA Hepatitis A Virus Isolate in Republic of Korea

Comparative analysis of disease severity between genotypes IA and IIIA of hepatitis A virus

Hepatitis A virus subgenotyping based on RT-qPCR assays

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